In vitro Hematopoietic and Endothelial Potential of flk-1-/-Embryonic Stem Cells and Embryos
Mice deficient in the Flk-1 receptor tyrosine kinase are known to die in utero because of defective vascular and hematopoietic development. Here, we show that flk-1-/-embryonic stem cells are nevertheless able to differentiate into hematopoietic and endothelial cells in vitro, although they give ris...
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Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1999-03, Vol.96 (5), p.2159-2164 |
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Sprache: | eng |
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Zusammenfassung: | Mice deficient in the Flk-1 receptor tyrosine kinase are known to die in utero because of defective vascular and hematopoietic development. Here, we show that flk-1-/-embryonic stem cells are nevertheless able to differentiate into hematopoietic and endothelial cells in vitro, although they give rise to a greatly reduced number of blast colonies, a measure of hemangioblast potential. Furthermore, normal numbers of hematopoietic progenitors are found in 7.5-day postcoitum flk-1-/-embryos, even though 8.5-day postcoitum flk-1-/-embryos are known to be deficient in such cells. Our results suggest that hematopoietic/endothelial progenitors arise independently of Flk-1, but that their subsequent migration and expansion require a Flk-1-mediated signal. |
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ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.96.5.2159 |