In vitro Hematopoietic and Endothelial Potential of flk-1-/-Embryonic Stem Cells and Embryos

Mice deficient in the Flk-1 receptor tyrosine kinase are known to die in utero because of defective vascular and hematopoietic development. Here, we show that flk-1-/-embryonic stem cells are nevertheless able to differentiate into hematopoietic and endothelial cells in vitro, although they give ris...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1999-03, Vol.96 (5), p.2159-2164
Hauptverfasser: Schuh, Andre C., Faloon, Patrick, Hu, Qing-Long, Bhimani, Munsif, Choi, Kyunghee
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Sprache:eng
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Zusammenfassung:Mice deficient in the Flk-1 receptor tyrosine kinase are known to die in utero because of defective vascular and hematopoietic development. Here, we show that flk-1-/-embryonic stem cells are nevertheless able to differentiate into hematopoietic and endothelial cells in vitro, although they give rise to a greatly reduced number of blast colonies, a measure of hemangioblast potential. Furthermore, normal numbers of hematopoietic progenitors are found in 7.5-day postcoitum flk-1-/-embryos, even though 8.5-day postcoitum flk-1-/-embryos are known to be deficient in such cells. Our results suggest that hematopoietic/endothelial progenitors arise independently of Flk-1, but that their subsequent migration and expansion require a Flk-1-mediated signal.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.96.5.2159