Lack of Tactile Pain (Allodynia) in Lipocalin-Type Prostaglandin D Synthase-Deficient Mice

Prostaglandin (PG) D2is the most abundant prostanoid produced in the central nervous system of mammals and has been implicated in the modulation of neural functions such as sleep induction, nociception, regulation of body temperature, and odor responses. We generated gene-knockout mice for lipocalin-type PGD2synthase (L-PGDS) and found that the intrathecal administration of PGE2an endogenous pain-producing substance, failed to elicit allodynia (touch-evoked pain), which is one typical phenomenon of neuropathic pain, whereas it evoked thermal hyperalgesia, in L-PGDS-/- mice. We also found that the allodynic response induced by the γ -aminobutiryc acid (GABA)Areceptor antagonist bicuculline was selectively abolished in the L-PGDS-/- mice, among excitatory and inhibitory agents that induced allodynia in wild-type mice. Interestingly, simultaneous injection of a femtogram amount of PGD2with PGE2or bicuculline induced allodynia in L-PGDS-/- mice to the same extent as in wild-type mice. The PGE2- or bicuculline-evoked allodynia in wild-type and in PGD2-supplemented L-PGDS-/- mice was blocked by a PGD2receptor antagonist given in a femtogram amount. These results reveal that endogenous PGD2is essential for both PGE2- and bicuculline-induced allodynia.