Molecular Interactions Between Interferon Consensus Sequence Binding Protein and Members of the Interferon Regulatory Factor Family

Interferon (IFN) consensus sequence binding protein (ICSBP) is a transcription factor expressed mostly in the cells of the immune system. ICSBP belongs to the IFN regulatory factor (IRF) family, which also includes IRF-1, IRF-2, and the IFN-α-stimulated gene factor 3γ (ISGF3γ). We show here that ICS...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1994-05, Vol.91 (11), p.5046-5050
Hauptverfasser: Bovolenta, Chiara, Driggers, Paul H., Marks, Michael S., Medin, Jeffrey A., Politis, Alexander D., Vogel, Stefanie N., Levy, David E., Sakaguchi, Kazuyasu, Appella, Ettore, Coligan, John E., Ozato, Keiko
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Sprache:eng
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Zusammenfassung:Interferon (IFN) consensus sequence binding protein (ICSBP) is a transcription factor expressed mostly in the cells of the immune system. ICSBP belongs to the IFN regulatory factor (IRF) family, which also includes IRF-1, IRF-2, and the IFN-α-stimulated gene factor 3γ (ISGF3γ). We show here that ICSBP forms a complex with IRF-1 or IRF-2 both in vivo and in vitro and, in the presence or absence of the target DNA, with the IFN-stimulated response element (ISRE). Further, electrophoretic mobility shift assays show that this interaction greatly enhances the otherwise very low binding affinity of ICSBP to the ISRE. We show, on the other hand, that ICSBP inhibits binding of the IFN-α-stimulated gene factor 3γ to the ISRE. Through these interactions ICSBP is likely to exert complex modulatory functions in the regulation of IFN-stimulated genes.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.91.11.5046