Identification and Preliminary Characterization of a Protein Motif Related to the Zinc Finger

We have identified a protein motif, related to the zinc finger, which defines a newly discovered family of proteins. The motif was found in the sequence of the human RING1 gene, which is proximal to the major histocompatibility complex region on chromosome six. We propose naming this motif the "...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1993-03, Vol.90 (6), p.2112-2116
Hauptverfasser:	Lovering, Ruth, Hanson, Isabel M., Borden, Katherine L., Martin, Stephen, O'Reilly, Nicola J., Evan, Gerard I., Rahman, Dinah, Darryl J. C. Pappin, Trowsdale, John, Freemont, Paul S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino acids Analytical, structural and metabolic biochemistry Animals Base Sequence binding Binding and carrier proteins Biochemistry Biological and medical sciences Cell Line Cell lines Cobalt Cobalt - metabolism Deoxyribonucleic acid DNA DNA - genetics DNA - metabolism DNA-Binding Proteins - chemistry DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism domains Fundamental and applied biological sciences. Psychology Generally accepted auditing standards Genes HeLa Cells Humans identification Ligands man Molecular Sequence Data Nucleic acids Oncogenes peptides Poly A - genetics Poly A - isolation & purification Polycomb Repressive Complex 1 Protein Binding Protein Structure, Secondary Proteins RING1 gene RNA - genetics RNA - isolation & purification RNA, Messenger Scientific imaging Sequence Homology, Amino Acid Spectrophotometry synthetic Tumor Cells, Cultured Zinc Zinc - metabolism zinc finger Zinc Fingers - genetics
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Zusammenfassung:	We have identified a protein motif, related to the zinc finger, which defines a newly discovered family of proteins. The motif was found in the sequence of the human RING1 gene, which is proximal to the major histocompatibility complex region on chromosome six. We propose naming this motif the "RING finger" and it is found in 27 proteins, all of which have putative DNA binding functions. We have synthesized a peptide corresponding to the RING1 motif and examined a number of properties, including metal and DNA binding. We provide evidence to support the suggestion that the RING finger motif is the DNA binding domain of this newly defined family of proteins.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.90.6.2112