Crystal structure and sequence-dependent conformation of the A.G mispaired oligonucleotide d(CGCAAGCTGGCG)

The crystal structure of the dodecanucleotide d(CGCAAGCTGGCG) has been determined to a resolution of 2.5 A and refined to an R factor of 19.3% for 1710 reflections. The sequence crystallizes as a B-type double helix, with two G(anti).A(syn) base pairs. These are stabilized by three-center hydrogen b...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1990-09, Vol.87 (17), p.6693-6697
Hauptverfasser:	WEBSTER, G. D, SANDERSON, M. R, SKELLY, J. V, NEIDLE, S, SWANN, P. F, LI, B. F, TICKLE, I. J
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenine Base Composition Base Sequence Biological and medical sciences Crystalline structure Crystallization Fundamental and applied biological sciences. Psychology Guanine Hydrogen Bonding Models, Molecular Molecular biophysics Molecular Sequence Data Nucleic Acid Conformation Oligodeoxyribonucleotides Structure in molecular biology
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Beschreibung
Zusammenfassung:	The crystal structure of the dodecanucleotide d(CGCAAGCTGGCG) has been determined to a resolution of 2.5 A and refined to an R factor of 19.3% for 1710 reflections. The sequence crystallizes as a B-type double helix, with two G(anti).A(syn) base pairs. These are stabilized by three-center hydrogen bonds to pyrimidines that induce perturbations in base-pair geometry. The central AGCT region of the helix has a wide (greater than 6 A) minor groove.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.87.17.6693