A Nuclear Protein Associated with Human Cancer Cells Binds Preferentially to a Human Repetitive DNA Sequence

A protein (Rp66) of 66 kDa was shown by DNA-binding protein blot assay to bind to a human repetitive DNA sequence (low-repeat sequence; LRS) in each of 10 transformed human cell lines examined. This protein-DNA interaction was not observed in 11 normal human cell cultures or in the Chinese hamster c...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1989-11, Vol.86 (21), p.8472-8476
Hauptverfasser:	Law, Martha Liao, Gao, Jizong, Puck, Theodore T.
Format:	Artikel
Sprache:	eng
Schlagworte:	550201 - Biochemistry- Tracer Techniques ANIMAL CELLS Animals AUTORADIOGRAPHY BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES Biological and medical sciences Cell culture techniques CELL DIFFERENTIATION Cell Line Cell lines Cell physiology Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes Cellular differentiation CHEMICAL REACTIONS CHO CELLS CONNECTIVE TISSUE CELLS Cricetinae CROSS-LINKING DAYS LIVING RADIOISOTOPES DISEASES DNA DNA binding proteins DNA-Binding Proteins - isolation & purification DNA-Binding Proteins - metabolism ELECTROPHORESIS Electrophoresis, Polyacrylamide Gel FIBROBLASTS Fundamental and applied biological sciences. Psychology Gels Genomes Humans IMMUNE SYSTEM DISEASES ISOTOPES LIGHT NUCLEI LYMPHOMAS Molecular and cellular biology Molecular Weight Neoplasm Proteins - isolation & purification Neoplasm Proteins - metabolism NEOPLASMS Nuclear Proteins - isolation & purification Nuclear Proteins - metabolism NUCLEI NUCLEIC ACIDS NUCLEOPROTEINS Nucleotide sequences ODD-ODD NUCLEI ORGANIC COMPOUNDS PHOSPHORUS 32 PHOSPHORUS ISOTOPES POLYMERIZATION PROTEINS PURIFICATION RADIOISOTOPES repeated sequence Repetitive Sequences, Nucleic Acid Rp66 protein SOMATIC CELLS Tetradecanoylphorbol Acetate - pharmacology Transformed cell line transformed cells Tumor cell line TUMOR CELLS
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Zusammenfassung:	A protein (Rp66) of 66 kDa was shown by DNA-binding protein blot assay to bind to a human repetitive DNA sequence (low-repeat sequence; LRS) in each of 10 transformed human cell lines examined. This protein-DNA interaction was not observed in 11 normal human cell cultures or in the Chinese hamster cell line CHO-K1. Gel retardation assay confirmed the specificity of the protein-DNA binding between Rp66 and LRS. In a histiocytic lymphoma human cell line, U937, that can be induced to differentiate in the presence of phorbol ester, this binding disappeared after cell differentiation. These together with other results cited suggest a regulatory role for these repetitive sequences in the human genome, with particular application to cancer.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.86.21.8472