Reconstitution of an Inwardly Rectifying Potassium Channel from the Basolateral Membranes of Necturus Enterocytes into Planar Lipid Bilayers

Basolateral membrane vesicles from Necturus enterocytes, highly (>20-fold) enriched in Na+,K+-ATPase, were reconstituted into planar lipid bilayers. The principal channel activity observed is selective for K+over Na+and Cl-. This K+channel is blocked by Ba2+and Leiurus quinquestriatus venom but i...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1989-07, Vol.86 (13), p.5212-5216
Hauptverfasser:	Costantin, James, Alcalen, Socorro, Angela De Souza Otero, Dubinsky, William P., Schultz, Stanley G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Barium - pharmacology Biological and medical sciences Cell Fractionation Cell Membrane - physiology Cell Membrane - ultrastructure Cell membranes Cell physiology Depolarization Electric potential Enterocytes Epithelial cells Fundamental and applied biological sciences. Psychology Intestinal Mucosa - physiology Intestine, Small - physiology Ion channels Kinetics Lipid Bilayers Male Membrane and intracellular transports Molecular and cellular biology Necturus Necturus maculosa P branes Phosphatases Potassium Channels - drug effects Potassium Channels - physiology reconstitution Small intestine Venoms
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Beschreibung
Zusammenfassung:	Basolateral membrane vesicles from Necturus enterocytes, highly (>20-fold) enriched in Na+,K+-ATPase, were reconstituted into planar lipid bilayers. The principal channel activity observed is selective for K+over Na+and Cl-. This K+channel is blocked by Ba2+and Leiurus quinquestriatus venom but is not affected by Ca2+over the range of 10-3to
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.86.13.5212