Induction of c-sis mRNA and Activity Similar to Platelet-Derived Growth Factor by Transforming Growth Factor β : A Proposed Model for Indirect Mitogenesis Involving Autocrine Activity

Treatment of quiescent cultures of mouse embryo-derived AKR-2B cells with transforming growth factor β resulted in an early induction of c-sis mRNA. The increase in c-sis mRNA was followed by a corresponding increase in protein similar to platelet-derived growth factor (PDGF) in the culture medium....

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1986-04, Vol.83 (8), p.2453-2457
Hauptverfasser:	Leof, Edward B., Proper, Jacqueline A., Goustin, Anton Scott, Shipley, Gary D., DiCorleto, Paul E., Moses, Harold L.
Format:	Artikel
Sprache:	eng
Schlagworte:	3T3 cells Animals Biological and medical sciences Cell culture techniques Cell Cycle - drug effects Cell growth Cell physiology Cells Cells, Cultured Cultured cells Epithelial cells Fundamental and applied biological sciences. Psychology Gene Expression Regulation - drug effects Humans Kinetics Messenger RNA Mice Mitosis - drug effects Molecular and cellular biology Peptides - pharmacology Platelet-Derived Growth Factor - biosynthesis Platelet-Derived Growth Factor - genetics Proto-Oncogene Proteins - genetics Proto-Oncogenes Receptors, Cell Surface - metabolism Receptors, Platelet-Derived Growth Factor Responses to growth factors, tumor promotors, other factors RNA sis genes Transcription, Genetic - drug effects Transforming Growth Factors
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Zusammenfassung:	Treatment of quiescent cultures of mouse embryo-derived AKR-2B cells with transforming growth factor β resulted in an early induction of c-sis mRNA. The increase in c-sis mRNA was followed by a corresponding increase in protein similar to platelet-derived growth factor (PDGF) in the culture medium. In addition, PDGF-regulated genes (c-fos and c-myc) were stimulated by transforming growth factor β with delayed kinetics relative to that seen in other cell systems with direct PDGF stimulation. A model is proposed in which the monolayer mitogenicity of transforming growth factor β is mediated by the induction of c-sis and PDGF and the subsequent autocrine stimulation of c-fos, c-myc, and other PDGF-inducible genes.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.83.8.2453