Characterization of a Porcine Genomic Clone Encoding a Major Histocompatibility Antigen: Expression in Mouse L Cells

A porcine genomic clone encoding a major histocompatibility (MHC) antigen was isolated by direct screening of a swine genomic library with a heterologous human MHC cDNA probe. Mouse L cells transformed with DNA from the clone stably express swine MHC antigen. Pig alloantisera specifically lyse trans...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1982-03, Vol.79 (5), p.1403-1407
Hauptverfasser:	Singer, Dinah S., Camerini-Otero, R. Daniel, Satz, M. Leonardo, Osborne, Barbara, Sachs, David, Rudikoff, Stuart
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antigens Bacteriophage lambda - genetics Cell lines Cloning, Molecular Complementary DNA Delta cells DNA DNA probes Gene Expression Regulation Genes Haplotypes Histocompatibility Antigens - genetics HLA antigens L cells L Cells (Cell Line) Mice Nucleic Acid Hybridization Swine - genetics Swine - immunology Transformed cell line
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Beschreibung
Zusammenfassung:	A porcine genomic clone encoding a major histocompatibility (MHC) antigen was isolated by direct screening of a swine genomic library with a heterologous human MHC cDNA probe. Mouse L cells transformed with DNA from the clone stably express swine MHC antigen. Pig alloantisera specifically lyse transformant but not control cell lines in a complement-mediated cytotoxicity assay. Direct immunoprecipitation of radiolabeled cellular protein from transformation lines by pig alloantiserum results in the coprecipitation of swine MHC heavy chain and mouse β2-microglobulin, demonstrating the association of heterologous subunits of MHC antigens.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.79.5.1403