Interaction of Phalloidin with Actin

Phalloidin, a toxic bicyclic peptide of rapid action from the toadstool, Amanita phalloides, gives rise to polymerization of G-actin to filamentous structures (Ph-actin) in a medium of low ionic strength. Ph-actin closely resembles the microfilaments found in liver membrane fractions (Ph-filaments)...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1974-07, Vol.71 (7), p.2803-2807
Hauptverfasser:	Lengsfeld, Anneliese M., Low, Irmentraut, Wieland, Theodor, Dancker, Peter, Hasselbach, Wilhelm
Format:	Artikel
Sprache:	eng
Schlagworte:	Actins Actins - metabolism Basidiomycota Biological Sciences: Cell Biology Cell Fractionation Cell Membrane - drug effects Cell membranes Cells Cytochalasin B - pharmacology Cytochalasins Electron microscopes Inclusion Bodies - drug effects Liver Liver - drug effects Liver cells Microfilaments Mycotoxins - metabolism Myosin Subfragments - pharmacology P branes Peptides, Cyclic - metabolism Plant Extracts Polymerization Potassium Iodide - pharmacology
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Beschreibung
Zusammenfassung:	Phalloidin, a toxic bicyclic peptide of rapid action from the toadstool, Amanita phalloides, gives rise to polymerization of G-actin to filamentous structures (Ph-actin) in a medium of low ionic strength. Ph-actin closely resembles the microfilaments found in liver membrane fractions (Ph-filaments) after in vivo or in vitro poisoning. Both phalloidin induced filaments are resistant to 0.6 M KI in contrast to F-actin, and become decorated by heavy meromyosin. After preincubation with cytochalasin B significantly fewer actin filaments are observed.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.71.7.2803