Reciprocal Inhibition of Mouse Leukemia Virus Infection by Fv-1 Allele Cell Extracts
Soluble extracts of mouse cells with Fv-1nor Fv-1bgene alleles specifically and reciprocally inhibit infection of B- or N-tropic mouse leukemia viruses in permissive cell cultures. NB-tropic virus infection was not inhibited by either cell extract. Extracts from Fv-1-cells did not inhibit infection...
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Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1974-10, Vol.71 (10), p.4241-4245 |
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Sprache: | eng |
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Zusammenfassung: | Soluble extracts of mouse cells with Fv-1nor Fv-1bgene alleles specifically and reciprocally inhibit infection of B- or N-tropic mouse leukemia viruses in permissive cell cultures. NB-tropic virus infection was not inhibited by either cell extract. Extracts from Fv-1-cells did not inhibit infection by the three virus host-range types, but N- or B-tropic virus infection of Fv-1-cells was inhibited by extracts of the nonpermissive cells, and Fv-1nbcell extracts inhibited both viruses. The maximum degree of inhibition was 50-80% as determined by immunofluorescent or plaque assays, with extracts containing up to 500 μ g/ml of nonpermissive cell protein. The inhibitor(s) is relatively unstable since activity is lost after 2 hr at 37 degrees or 30 min at 56 degrees. The inhibitor(s) was most effective if added 2 hr before or within 2 hr after infection, did not react with the virus directly, inhibit virus attachment, or inhibit the normal cell functions tested. These results indicate that nonpermissive mouse cells contain a product, possibly determined by the Fv-1 gene, which inhibits some early postpenetration event(s) in leukemia virus infection. |
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ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.71.10.4241 |