An RNA that Multiplies Indefinitely with DNA-Dependent RNA Polymerase: Selection from a Random Copolymer

We have selected from a mixture of random polynucleotides an RNA that is able to replicate in the presence of the DNA-dependent RNA polymerase of Escherichia coli, provided the medium contains Mn2+and ITP, in addition to ATP, CTP, and UTP. The RNA consists of a chain of about 125 (± 25) nucleotides...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1973-03, Vol.70 (3), p.934-938
Hauptverfasser:	Biebricher, Christof K., Orgel, Leslie E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - metabolism Biological Sciences: Biochemistry Carbon Isotopes Copolymers Cytosine Nucleotides - metabolism DNA DNA-Directed RNA Polymerases - metabolism Enzymes Escherichia coli Escherichia coli - enzymology Inosine Nucleotides - metabolism Manganese - pharmacology Molecules Nucleotides Nucleotides - analysis Phosphorus Isotopes Polymers Polynucleotides Ribonucleases - metabolism RNA RNA - analysis RNA - biosynthesis RNA - metabolism Space life sciences Templates, Genetic Transfer RNA Tritium Uracil Nucleotides - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	We have selected from a mixture of random polynucleotides an RNA that is able to replicate in the presence of the DNA-dependent RNA polymerase of Escherichia coli, provided the medium contains Mn2+and ITP, in addition to ATP, CTP, and UTP. The RNA consists of a chain of about 125 (± 25) nucleotides and appears to have a defined, nonrepetitive sequence.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.70.3.934