TRANSFER RNA, III. RECONSTITUTION OF ALANINE ACCEPTOR ACTIVITY FROM FRAGMENTS PRODUCED BY SPECIFIC CLEAVAGE OF tRNAAlaII AT ITS ANTICODON

This report describes experiments showing that tRNA Ala II can be cleaved specifically at the G residue of its anticodon to give “half” molecules in high yield. Neither of these fragments has alanine-acceptor activity, but this activity can be reconstituted by mixing the fragments in the presence of...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1969-04, Vol.62 (4), p.1203-1209
Hauptverfasser: Imura, Nobumasa, Schwam, Harvey, Chambers, Robert W.
Format: Artikel
Sprache:eng
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Zusammenfassung:This report describes experiments showing that tRNA Ala II can be cleaved specifically at the G residue of its anticodon to give “half” molecules in high yield. Neither of these fragments has alanine-acceptor activity, but this activity can be reconstituted by mixing the fragments in the presence of Mg +2 . In dilute salt, on the other hand, the active complex dissociates spontaneously at 25° into the two fragments. Thus, both “halves” of tRNA Ala II are necessary for acceptor activity and, in the presence of Mg +2 , combination of these fragments to give an active duplex is thermodynamically favorable.
ISSN:0027-8424
1091-6490