Physiological Role of 70S Ribosomes in Bacteria

Evidence is presented indicating that free 70S ribosomes are real components of Bacillus stearothermophilus and Escherichia coli in exponential and stationary phases of growth. After pulses of radioactive leucine or uracil were given, protein synthesis was instantaneously stopped by chloramphenicol,...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1969-02, Vol.62 (2), p.574-580
Hauptverfasser:	Algranati, Israel D., Gonzalez, Nelida S., Bade, Ernesto G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus - growth & development Bacillus - metabolism Bacteria Bacterial Proteins - biosynthesis Biochemistry Biological Sciences: Biochemistry Carbon Isotopes Cell Division Cell growth Centrifugation, Density Gradient Chloramphenicol - pharmacology Cold Temperature Cooling Escherichia coli - growth & development Escherichia coli - metabolism Leucine - metabolism Messenger RNA Monomers Peptides - metabolism Polyribosomes Protein synthesis Ribonucleases Ribosomes Ribosomes - physiology RNA, Bacterial RNA, Messenger Steepest descent method Uracil - metabolism
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Beschreibung
Zusammenfassung:	Evidence is presented indicating that free 70S ribosomes are real components of Bacillus stearothermophilus and Escherichia coli in exponential and stationary phases of growth. After pulses of radioactive leucine or uracil were given, protein synthesis was instantaneously stopped by chloramphenicol, or polypeptide growth was allowed to proceed to completion in slowly cooled cells. Analysis of label distribution in sucrose gradients showed that 70S monomers, and not subunits, are final products of translation. A ribosome cycle for protein synthesis in bacteria is proposed.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.62.2.574