High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats
Foods high in resistant starch have the potential to improve human health and lower the risk of serious noninfectious diseases. RNA interference was used to down-regulate the two different isoforms of starch-branching enzyme (SBE) II (SBEIIa and SBEIIb) in wheat endosperm to raise its amylose conten...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2006-03, Vol.103 (10), p.3546-3551 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3551 |
|---|---|
| container_issue | 10 |
| container_start_page | 3546 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 103 |
| creator | Regina, A Bird, A Topping, D Bowden, S Freeman, J Barsby, T Kosar-Hashemi, B Li, Z Rahman, S Morell, M |
| description | Foods high in resistant starch have the potential to improve human health and lower the risk of serious noninfectious diseases. RNA interference was used to down-regulate the two different isoforms of starch-branching enzyme (SBE) II (SBEIIa and SBEIIb) in wheat endosperm to raise its amylose content. Suppression of SBEIIb expression alone had no effect on amylose content; however, suppression of both SBEIIa and SBEIIb expression resulted in starch containing >70% amylose. When the >70% amylose wheat grain was fed to rats in a diet as a wholemeal, several indices of large-bowel function, including short-chain fatty acids, were improved relative to standard wholemeal wheat. These results indicate that this high-amylose wheat has a significant potential to improve human health through its resistant starch content. |
| doi_str_mv | 10.1073/pnas.0510737103 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_pnas_</sourceid><recordid>TN_cdi_pnas_primary_103_10_3546_fulltext</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>30048624</jstor_id><sourcerecordid>30048624</sourcerecordid><originalsourceid>FETCH-LOGICAL-c618t-76f17c9ea0cf87f4ce14db22369328ecc64de746c50d961a2fec6a1c1b613b393</originalsourceid><addsrcrecordid>eNqFkUFv1DAQRiMEotvCmRMQcUBc0s7Yjp1ckKoKKFIFEtCz5TiTbFZJvNjZlv33dbqrLnCAky3Pm6cZf0nyAuEUQfGz9WjCKeTzXSHwR8kCocRMihIeJwsAprJCMHGUHIewAoAyL-BpcoQy50oIvkj0ZdcuMzNsexcovV2SmdKWRvJmojqttum3L-dpN07kG_I0Wkq7Ye3dDYX4Wnc2nq5Je-Nbyip3S30aFf20jNU0OsKz5Elj-kDP9-dJcv3xw4-Ly-zq66fPF-dXmZVYTJmSDSpbkgHbFKoRllDUFWNclpwVZK0UNSkhbQ51KdGwhqw0aLGSyCte8pPk_c673lQD1ZbGyZter303GL_VznT6z8rYLXXrbjSKHJBBFLzdC7z7uaEw6aELlvrejOQ2QUulcqVK8V8QFTIs2Qy--QtcuY0f4y9oBsihLAqM0NkOst6F4Kl5GBlBz7HqOWJ9iDh2vPp90wO_zzQC7_bA3HnQ8VnJcyF1s-n7iX5NEX39bzQSL3fEKkzOPyAcQBTyfsm9oTFOm9Z3QV9_v18vDoxMKH4HvI7N2A</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>201309881</pqid></control><display><type>article</type><title>High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats</title><source>MEDLINE</source><source>JSTOR Archive Collection A-Z Listing</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Regina, A ; Bird, A ; Topping, D ; Bowden, S ; Freeman, J ; Barsby, T ; Kosar-Hashemi, B ; Li, Z ; Rahman, S ; Morell, M</creator><creatorcontrib>Regina, A ; Bird, A ; Topping, D ; Bowden, S ; Freeman, J ; Barsby, T ; Kosar-Hashemi, B ; Li, Z ; Rahman, S ; Morell, M</creatorcontrib><description>Foods high in resistant starch have the potential to improve human health and lower the risk of serious noninfectious diseases. RNA interference was used to down-regulate the two different isoforms of starch-branching enzyme (SBE) II (SBEIIa and SBEIIb) in wheat endosperm to raise its amylose content. Suppression of SBEIIb expression alone had no effect on amylose content; however, suppression of both SBEIIa and SBEIIb expression resulted in starch containing >70% amylose. When the >70% amylose wheat grain was fed to rats in a diet as a wholemeal, several indices of large-bowel function, including short-chain fatty acids, were improved relative to standard wholemeal wheat. These results indicate that this high-amylose wheat has a significant potential to improve human health through its resistant starch content.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.0510737103</identifier><identifier>PMID: 16537443</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>1,4-alpha-Glucan Branching Enzyme - antagonists & inhibitors ; 1,4-alpha-Glucan Branching Enzyme - genetics ; 1,4-alpha-Glucan Branching Enzyme - metabolism ; amylose ; Amylose - metabolism ; Animals ; Biological Sciences ; Biology ; carbohydrate content ; Carbohydrates ; cecum ; Corn ; Diet ; Digesta ; digestion ; Endosperm ; Enzymes ; Fatty acids ; food intake ; Food, Genetically Modified ; gastrointesinal health ; gene silencing ; Genetic Engineering ; genetically modified foods ; Glucans - chemistry ; Health ; Health Status ; hexosyltransferases ; Humans ; Industrial research ; Intestine, Large - metabolism ; Isoenzymes - antagonists & inhibitors ; Isoenzymes - genetics ; Isoenzymes - metabolism ; large intestine ; Male ; Plants, Genetically Modified ; Protein isoforms ; Rats ; Rats, Sprague-Dawley ; Regional identity ; Ribonucleic acid ; RNA ; RNA Interference ; Rodents ; short chain fatty acids ; Small intestine ; Starch - chemistry ; Starch - metabolism ; starch branching enzyme ; Starches ; Transgenic plants ; Triticum - genetics ; Triticum - metabolism ; Triticum aestivum ; Wheat ; wheat starch</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2006-03, Vol.103 (10), p.3546-3551</ispartof><rights>Copyright 2006 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Mar 7, 2006</rights><rights>2006 by The National Academy of Sciences of the USA 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c618t-76f17c9ea0cf87f4ce14db22369328ecc64de746c50d961a2fec6a1c1b613b393</citedby><cites>FETCH-LOGICAL-c618t-76f17c9ea0cf87f4ce14db22369328ecc64de746c50d961a2fec6a1c1b613b393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/103/10.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30048624$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30048624$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27923,27924,53790,53792,58016,58249</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16537443$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Regina, A</creatorcontrib><creatorcontrib>Bird, A</creatorcontrib><creatorcontrib>Topping, D</creatorcontrib><creatorcontrib>Bowden, S</creatorcontrib><creatorcontrib>Freeman, J</creatorcontrib><creatorcontrib>Barsby, T</creatorcontrib><creatorcontrib>Kosar-Hashemi, B</creatorcontrib><creatorcontrib>Li, Z</creatorcontrib><creatorcontrib>Rahman, S</creatorcontrib><creatorcontrib>Morell, M</creatorcontrib><title>High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Foods high in resistant starch have the potential to improve human health and lower the risk of serious noninfectious diseases. RNA interference was used to down-regulate the two different isoforms of starch-branching enzyme (SBE) II (SBEIIa and SBEIIb) in wheat endosperm to raise its amylose content. Suppression of SBEIIb expression alone had no effect on amylose content; however, suppression of both SBEIIa and SBEIIb expression resulted in starch containing >70% amylose. When the >70% amylose wheat grain was fed to rats in a diet as a wholemeal, several indices of large-bowel function, including short-chain fatty acids, were improved relative to standard wholemeal wheat. These results indicate that this high-amylose wheat has a significant potential to improve human health through its resistant starch content.</description><subject>1,4-alpha-Glucan Branching Enzyme - antagonists & inhibitors</subject><subject>1,4-alpha-Glucan Branching Enzyme - genetics</subject><subject>1,4-alpha-Glucan Branching Enzyme - metabolism</subject><subject>amylose</subject><subject>Amylose - metabolism</subject><subject>Animals</subject><subject>Biological Sciences</subject><subject>Biology</subject><subject>carbohydrate content</subject><subject>Carbohydrates</subject><subject>cecum</subject><subject>Corn</subject><subject>Diet</subject><subject>Digesta</subject><subject>digestion</subject><subject>Endosperm</subject><subject>Enzymes</subject><subject>Fatty acids</subject><subject>food intake</subject><subject>Food, Genetically Modified</subject><subject>gastrointesinal health</subject><subject>gene silencing</subject><subject>Genetic Engineering</subject><subject>genetically modified foods</subject><subject>Glucans - chemistry</subject><subject>Health</subject><subject>Health Status</subject><subject>hexosyltransferases</subject><subject>Humans</subject><subject>Industrial research</subject><subject>Intestine, Large - metabolism</subject><subject>Isoenzymes - antagonists & inhibitors</subject><subject>Isoenzymes - genetics</subject><subject>Isoenzymes - metabolism</subject><subject>large intestine</subject><subject>Male</subject><subject>Plants, Genetically Modified</subject><subject>Protein isoforms</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Regional identity</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA Interference</subject><subject>Rodents</subject><subject>short chain fatty acids</subject><subject>Small intestine</subject><subject>Starch - chemistry</subject><subject>Starch - metabolism</subject><subject>starch branching enzyme</subject><subject>Starches</subject><subject>Transgenic plants</subject><subject>Triticum - genetics</subject><subject>Triticum - metabolism</subject><subject>Triticum aestivum</subject><subject>Wheat</subject><subject>wheat starch</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQRiMEotvCmRMQcUBc0s7Yjp1ckKoKKFIFEtCz5TiTbFZJvNjZlv33dbqrLnCAky3Pm6cZf0nyAuEUQfGz9WjCKeTzXSHwR8kCocRMihIeJwsAprJCMHGUHIewAoAyL-BpcoQy50oIvkj0ZdcuMzNsexcovV2SmdKWRvJmojqttum3L-dpN07kG_I0Wkq7Ye3dDYX4Wnc2nq5Je-Nbyip3S30aFf20jNU0OsKz5Elj-kDP9-dJcv3xw4-Ly-zq66fPF-dXmZVYTJmSDSpbkgHbFKoRllDUFWNclpwVZK0UNSkhbQ51KdGwhqw0aLGSyCte8pPk_c673lQD1ZbGyZter303GL_VznT6z8rYLXXrbjSKHJBBFLzdC7z7uaEw6aELlvrejOQ2QUulcqVK8V8QFTIs2Qy--QtcuY0f4y9oBsihLAqM0NkOst6F4Kl5GBlBz7HqOWJ9iDh2vPp90wO_zzQC7_bA3HnQ8VnJcyF1s-n7iX5NEX39bzQSL3fEKkzOPyAcQBTyfsm9oTFOm9Z3QV9_v18vDoxMKH4HvI7N2A</recordid><startdate>20060307</startdate><enddate>20060307</enddate><creator>Regina, A</creator><creator>Bird, A</creator><creator>Topping, D</creator><creator>Bowden, S</creator><creator>Freeman, J</creator><creator>Barsby, T</creator><creator>Kosar-Hashemi, B</creator><creator>Li, Z</creator><creator>Rahman, S</creator><creator>Morell, M</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20060307</creationdate><title>High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats</title><author>Regina, A ; Bird, A ; Topping, D ; Bowden, S ; Freeman, J ; Barsby, T ; Kosar-Hashemi, B ; Li, Z ; Rahman, S ; Morell, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c618t-76f17c9ea0cf87f4ce14db22369328ecc64de746c50d961a2fec6a1c1b613b393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>1,4-alpha-Glucan Branching Enzyme - antagonists & inhibitors</topic><topic>1,4-alpha-Glucan Branching Enzyme - genetics</topic><topic>1,4-alpha-Glucan Branching Enzyme - metabolism</topic><topic>amylose</topic><topic>Amylose - metabolism</topic><topic>Animals</topic><topic>Biological Sciences</topic><topic>Biology</topic><topic>carbohydrate content</topic><topic>Carbohydrates</topic><topic>cecum</topic><topic>Corn</topic><topic>Diet</topic><topic>Digesta</topic><topic>digestion</topic><topic>Endosperm</topic><topic>Enzymes</topic><topic>Fatty acids</topic><topic>food intake</topic><topic>Food, Genetically Modified</topic><topic>gastrointesinal health</topic><topic>gene silencing</topic><topic>Genetic Engineering</topic><topic>genetically modified foods</topic><topic>Glucans - chemistry</topic><topic>Health</topic><topic>Health Status</topic><topic>hexosyltransferases</topic><topic>Humans</topic><topic>Industrial research</topic><topic>Intestine, Large - metabolism</topic><topic>Isoenzymes - antagonists & inhibitors</topic><topic>Isoenzymes - genetics</topic><topic>Isoenzymes - metabolism</topic><topic>large intestine</topic><topic>Male</topic><topic>Plants, Genetically Modified</topic><topic>Protein isoforms</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Regional identity</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA Interference</topic><topic>Rodents</topic><topic>short chain fatty acids</topic><topic>Small intestine</topic><topic>Starch - chemistry</topic><topic>Starch - metabolism</topic><topic>starch branching enzyme</topic><topic>Starches</topic><topic>Transgenic plants</topic><topic>Triticum - genetics</topic><topic>Triticum - metabolism</topic><topic>Triticum aestivum</topic><topic>Wheat</topic><topic>wheat starch</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Regina, A</creatorcontrib><creatorcontrib>Bird, A</creatorcontrib><creatorcontrib>Topping, D</creatorcontrib><creatorcontrib>Bowden, S</creatorcontrib><creatorcontrib>Freeman, J</creatorcontrib><creatorcontrib>Barsby, T</creatorcontrib><creatorcontrib>Kosar-Hashemi, B</creatorcontrib><creatorcontrib>Li, Z</creatorcontrib><creatorcontrib>Rahman, S</creatorcontrib><creatorcontrib>Morell, M</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Regina, A</au><au>Bird, A</au><au>Topping, D</au><au>Bowden, S</au><au>Freeman, J</au><au>Barsby, T</au><au>Kosar-Hashemi, B</au><au>Li, Z</au><au>Rahman, S</au><au>Morell, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2006-03-07</date><risdate>2006</risdate><volume>103</volume><issue>10</issue><spage>3546</spage><epage>3551</epage><pages>3546-3551</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Foods high in resistant starch have the potential to improve human health and lower the risk of serious noninfectious diseases. RNA interference was used to down-regulate the two different isoforms of starch-branching enzyme (SBE) II (SBEIIa and SBEIIb) in wheat endosperm to raise its amylose content. Suppression of SBEIIb expression alone had no effect on amylose content; however, suppression of both SBEIIa and SBEIIb expression resulted in starch containing >70% amylose. When the >70% amylose wheat grain was fed to rats in a diet as a wholemeal, several indices of large-bowel function, including short-chain fatty acids, were improved relative to standard wholemeal wheat. These results indicate that this high-amylose wheat has a significant potential to improve human health through its resistant starch content.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>16537443</pmid><doi>10.1073/pnas.0510737103</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 2006-03, Vol.103 (10), p.3546-3551 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pnas_primary_103_10_3546_fulltext |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | 1,4-alpha-Glucan Branching Enzyme - antagonists & inhibitors 1,4-alpha-Glucan Branching Enzyme - genetics 1,4-alpha-Glucan Branching Enzyme - metabolism amylose Amylose - metabolism Animals Biological Sciences Biology carbohydrate content Carbohydrates cecum Corn Diet Digesta digestion Endosperm Enzymes Fatty acids food intake Food, Genetically Modified gastrointesinal health gene silencing Genetic Engineering genetically modified foods Glucans - chemistry Health Health Status hexosyltransferases Humans Industrial research Intestine, Large - metabolism Isoenzymes - antagonists & inhibitors Isoenzymes - genetics Isoenzymes - metabolism large intestine Male Plants, Genetically Modified Protein isoforms Rats Rats, Sprague-Dawley Regional identity Ribonucleic acid RNA RNA Interference Rodents short chain fatty acids Small intestine Starch - chemistry Starch - metabolism starch branching enzyme Starches Transgenic plants Triticum - genetics Triticum - metabolism Triticum aestivum Wheat wheat starch |
| title | High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T02%3A29%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pnas_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=High-amylose%20wheat%20generated%20by%20RNA%20interference%20improves%20indices%20of%20large-bowel%20health%20in%20rats&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Regina,%20A&rft.date=2006-03-07&rft.volume=103&rft.issue=10&rft.spage=3546&rft.epage=3551&rft.pages=3546-3551&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.0510737103&rft_dat=%3Cjstor_pnas_%3E30048624%3C/jstor_pnas_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=201309881&rft_id=info:pmid/16537443&rft_jstor_id=30048624&rfr_iscdi=true |