High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats
Foods high in resistant starch have the potential to improve human health and lower the risk of serious noninfectious diseases. RNA interference was used to down-regulate the two different isoforms of starch-branching enzyme (SBE) II (SBEIIa and SBEIIb) in wheat endosperm to raise its amylose conten...
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Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2006-03, Vol.103 (10), p.3546-3551 |
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Sprache: | eng |
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Zusammenfassung: | Foods high in resistant starch have the potential to improve human health and lower the risk of serious noninfectious diseases. RNA interference was used to down-regulate the two different isoforms of starch-branching enzyme (SBE) II (SBEIIa and SBEIIb) in wheat endosperm to raise its amylose content. Suppression of SBEIIb expression alone had no effect on amylose content; however, suppression of both SBEIIa and SBEIIb expression resulted in starch containing >70% amylose. When the >70% amylose wheat grain was fed to rats in a diet as a wholemeal, several indices of large-bowel function, including short-chain fatty acids, were improved relative to standard wholemeal wheat. These results indicate that this high-amylose wheat has a significant potential to improve human health through its resistant starch content. |
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ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.0510737103 |