CD47 expression in non-small cell lung cancer and its relationship with tumor-associated macrophage infiltration

Non-small cell lung cancer (NSCLC) has a high incidence, with most patients diagnosed beyond the optimal surgical window. Improving survival rates is critical to reducing lung cancer mortality, and identifying immune checkpoints is vital for prognosis stratification. To investigate the expression of CD47 in NSCLC and its relationship with tumor-associated macrophage infiltration. A retrospective analysis was conducted on 50 NSCLC patients treated between January 2014 and June 2018. Immunohistochemistry and confocal microscopy assessed CD47 expression in tumor and adjacent tissues, while immunofluorescence evaluated CD47 on tumor-infiltrating T lymphocytes. Kaplan-Meier survival analysis and Cox regression identified prognostic factors, and PLA technology examined CD47's interaction with VEGFR and CD36. CD47 positivity in tumor tissues (52%) was significantly higher than in adjacent tissues (20%) (P