simpleISM-A straight forward guide to upgrade from confocal to ISM

Resolution in a confocal laser scanning microscopes (CLSM) can be improved if the pinhole is closed. But closing the pinhole will deteriorate the signal to noise ratio (SNR). A simple technique to improve the SNR while keeping the resolution same by upgrading the system to an image scanning microsco...

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Veröffentlicht in:	PloS one 2022-12, Vol.17 (12), p.e0279378-e0279378
Hauptverfasser:	Goswami, Monalisa, Lachmann, René, Kretschmer, Robert, Heintzmann, Rainer
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Cameras Computer and Information Sciences Confocal microscopy Engineering and Technology Equipment and supplies Lab Protocol Laser applications Lasers Methods Microscope and microscopy Microscopes Microscopy, Confocal - methods Pinholes Product enhancement Research and Analysis Methods Resolution (Optics) Sensors Signal to noise ratio Synchronism Synchronization Telescopes Transistors
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Zusammenfassung:	Resolution in a confocal laser scanning microscopes (CLSM) can be improved if the pinhole is closed. But closing the pinhole will deteriorate the signal to noise ratio (SNR). A simple technique to improve the SNR while keeping the resolution same by upgrading the system to an image scanning microscope. In this paper, we explain in detail, based on an Olympus Fluoview 300 system, how a scanning microscope can be upgraded into an image scanning microscope (ISM) using a simple camera-based detector and an Arduino Due providing a galvo driving and camera synchronization signals. We could confirm a resolution improvement as well as superconcentration and made the interesting observation of a reduced influence of laser fluctuations.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0279378