Abundance of Phasi-Charoen-like virus in Aedes aegypti mosquito populations in different states of India
Mosquitoes are known to harbor a large number of insect specific viruses (ISV) in addition to viruses of public health importance. These ISVs are highly species specific and are non-pathogenic to humans or domestic animals. However, there is a potential threat of these ISVs evolving into human patho...
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description | Mosquitoes are known to harbor a large number of insect specific viruses (ISV) in addition to viruses of public health importance. These ISVs are highly species specific and are non-pathogenic to humans or domestic animals. However, there is a potential threat of these ISVs evolving into human pathogens by genome alterations. Some ISVs are known to modulate replication of pathogenic viruses by altering the susceptibility of vector mosquitoes to pathogenic viruses, thereby either inhibiting or enhancing transmission of the latter. In the present study, we report predominance of Phasi Charoen-like virus (PCLV, Family: Phenuviridae) contributing to >60% of the total reads in Aedes aegypti mosquitoes collected from Pune district of Maharashtra state using next generation sequencing based metagenomic analysis of viromes. Similar results were also obtained with mosquitoes from Assam, Tamil Nadu and Karnataka states of India. Comparison of Pune mosquito sequences with PCLV Rio (Brazil) isolate showed 98.90%, 99.027% and 98.88% homologies in the S, M and L segments respectively indicating less genetic heterogeneity of PCLV. The study also demonstrated occurrence of transovarial transmission as seen by detection of PCLV in eggs, larvae, pupae and male mosquitoes. Ae. aegypti mosquitoes collected from Pune also showed a large number of reads for viruses belonging to Baculoviridae, Rhabdoviridae, Genomoviridae and Bunyaviridae families. The role of PCLV in the replication of dengue and chikungunya virus is yet not clear. It warrants further studies to know the significance of PCLV and other ISVs on the replication and transmission of Ae. aegypti borne pathogenic viruses, especially in the absence of prophylactics or therapeutics. |
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These ISVs are highly species specific and are non-pathogenic to humans or domestic animals. However, there is a potential threat of these ISVs evolving into human pathogens by genome alterations. Some ISVs are known to modulate replication of pathogenic viruses by altering the susceptibility of vector mosquitoes to pathogenic viruses, thereby either inhibiting or enhancing transmission of the latter. In the present study, we report predominance of Phasi Charoen-like virus (PCLV, Family: Phenuviridae) contributing to >60% of the total reads in Aedes aegypti mosquitoes collected from Pune district of Maharashtra state using next generation sequencing based metagenomic analysis of viromes. Similar results were also obtained with mosquitoes from Assam, Tamil Nadu and Karnataka states of India. Comparison of Pune mosquito sequences with PCLV Rio (Brazil) isolate showed 98.90%, 99.027% and 98.88% homologies in the S, M and L segments respectively indicating less genetic heterogeneity of PCLV. The study also demonstrated occurrence of transovarial transmission as seen by detection of PCLV in eggs, larvae, pupae and male mosquitoes. Ae. aegypti mosquitoes collected from Pune also showed a large number of reads for viruses belonging to Baculoviridae, Rhabdoviridae, Genomoviridae and Bunyaviridae families. The role of PCLV in the replication of dengue and chikungunya virus is yet not clear. It warrants further studies to know the significance of PCLV and other ISVs on the replication and transmission of Ae. aegypti borne pathogenic viruses, especially in the absence of prophylactics or therapeutics.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0277276</identifier><identifier>PMID: 36490242</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adults ; Aedes ; Aedes aegypti ; Analysis ; Animals ; Annealing ; Biology and Life Sciences ; Chikungunya virus ; Culicidae ; Dengue fever ; Domestic animals ; Encephalitis ; Ethanol ; Genetic vectors ; Genomes ; Heterogeneity ; Humans ; India ; Infections ; Insect Viruses ; Insects ; Laboratories ; Larvae ; Malaria ; Male ; Medicine and Health Sciences ; Metagenomics ; Morbidity ; Mosquito Vectors ; Mosquitoes ; Next-generation sequencing ; People and Places ; Public health ; Replication ; Thermal cycling ; Tropical diseases ; Vaccines ; Vector-borne diseases ; Viruses ; West Nile virus ; Zika virus</subject><ispartof>PloS one, 2022-12, Vol.17 (12), p.e0277276-e0277276</ispartof><rights>Copyright: © 2022 Lole et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</rights><rights>COPYRIGHT 2022 Public Library of Science</rights><rights>2022 Lole et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 Lole et al 2022 Lole et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-edc81df6ae7890f6a4c4470dbeaffc657b90bbcc10c2e7742d819531dee722493</citedby><cites>FETCH-LOGICAL-c692t-edc81df6ae7890f6a4c4470dbeaffc657b90bbcc10c2e7742d819531dee722493</cites><orcidid>0000-0002-6188-3789</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733876/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733876/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36490242$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Lanz-Mendoza, Humberto</contributor><creatorcontrib>Lole, Kavita</creatorcontrib><creatorcontrib>Ramdasi, Ashwini</creatorcontrib><creatorcontrib>Patil, Sucheta</creatorcontrib><creatorcontrib>Thakar, Shivani</creatorcontrib><creatorcontrib>Nath, Amol</creatorcontrib><creatorcontrib>Ghuge, Onkar</creatorcontrib><creatorcontrib>Gangopadhayya, Abhranil</creatorcontrib><creatorcontrib>Sudeep, Anakkathil B</creatorcontrib><creatorcontrib>Cherian, Sarah</creatorcontrib><title>Abundance of Phasi-Charoen-like virus in Aedes aegypti mosquito populations in different states of India</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Mosquitoes are known to harbor a large number of insect specific viruses (ISV) in addition to viruses of public health importance. 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These ISVs are highly species specific and are non-pathogenic to humans or domestic animals. However, there is a potential threat of these ISVs evolving into human pathogens by genome alterations. Some ISVs are known to modulate replication of pathogenic viruses by altering the susceptibility of vector mosquitoes to pathogenic viruses, thereby either inhibiting or enhancing transmission of the latter. In the present study, we report predominance of Phasi Charoen-like virus (PCLV, Family: Phenuviridae) contributing to >60% of the total reads in Aedes aegypti mosquitoes collected from Pune district of Maharashtra state using next generation sequencing based metagenomic analysis of viromes. Similar results were also obtained with mosquitoes from Assam, Tamil Nadu and Karnataka states of India. Comparison of Pune mosquito sequences with PCLV Rio (Brazil) isolate showed 98.90%, 99.027% and 98.88% homologies in the S, M and L segments respectively indicating less genetic heterogeneity of PCLV. The study also demonstrated occurrence of transovarial transmission as seen by detection of PCLV in eggs, larvae, pupae and male mosquitoes. Ae. aegypti mosquitoes collected from Pune also showed a large number of reads for viruses belonging to Baculoviridae, Rhabdoviridae, Genomoviridae and Bunyaviridae families. The role of PCLV in the replication of dengue and chikungunya virus is yet not clear. It warrants further studies to know the significance of PCLV and other ISVs on the replication and transmission of Ae. aegypti borne pathogenic viruses, especially in the absence of prophylactics or therapeutics.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>36490242</pmid><doi>10.1371/journal.pone.0277276</doi><tpages>e0277276</tpages><orcidid>https://orcid.org/0000-0002-6188-3789</orcidid><oa>free_for_read</oa></addata></record> |
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source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
subjects | Adults Aedes Aedes aegypti Analysis Animals Annealing Biology and Life Sciences Chikungunya virus Culicidae Dengue fever Domestic animals Encephalitis Ethanol Genetic vectors Genomes Heterogeneity Humans India Infections Insect Viruses Insects Laboratories Larvae Malaria Male Medicine and Health Sciences Metagenomics Morbidity Mosquito Vectors Mosquitoes Next-generation sequencing People and Places Public health Replication Thermal cycling Tropical diseases Vaccines Vector-borne diseases Viruses West Nile virus Zika virus |
title | Abundance of Phasi-Charoen-like virus in Aedes aegypti mosquito populations in different states of India |
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