Erythro-VLPs: Anchoring SARS-CoV-2 spike proteins in erythrocyte liposomes

Novel therapeutic strategies are needed to control the SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) pandemic. Here, we present a protocol to anchor the SARS-CoV-2 spike (S-)protein in the cytoplasmic membranes of erythrocyte liposomes. A surfactant was used to stabilize the S-protein...

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Veröffentlicht in:	PloS one 2022-03, Vol.17 (3), p.e0263671-e0263671
Hauptverfasser:	Himbert, Sebastian, Gastaldo, Isabella Passos, Ahmed, Rashik, Pomier, Karla Martinez, Cowbrough, Braeden, Jahagirdar, Dushyant, Ros, Samantha, Juhasz, Janos, Stöver, Harald D H, Ortega, Joaquin, Melacini, Giuseppe, Bowdish, Dawn M E, Rheinstädter, Maikel C
Format:	Artikel
Sprache:	eng
Schlagworte:	ACE2 Angiotensin Angiotensin converting enzyme Angiotensin-converting enzyme 2 Antigen presenting cells Aqueous environments Aqueous solutions Assaying Astronomy Biochemistry Biology Biology and Life Sciences Chemistry Chromatography Coronaviruses COVID-19 COVID-19 vaccines Cytoplasmic membranes Disease transmission Enzyme-linked immunosorbent assay Enzymes Erythrocytes Fluorescence Hydrocarbons Immunoassays Immunology Insertion Interferometry Light scattering Liposomes Medicine and health sciences Membranes Molecular dynamics Pandemics Peptidyl-dipeptidase A Photon correlation spectroscopy Physical Sciences Physics Protein structure Proteins Research and Analysis Methods Seroconversion Severe acute respiratory syndrome Severe acute respiratory syndrome coronavirus 2 Simulation Surfactants Viral diseases Viruses
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