Chorioallantoic membrane (CAM) assay to study treatment effects in diffuse intrinsic pontine glioma

Diffuse intrinsic pontine glioma (DIPG) is a lethal pediatric brain tumor. While there are a number of in vivo rodent models for evaluating tumor biology and response to therapy, these models require significant time and resources. Here, we established the chick-embryo chorioallantoic (CAM) assay as...

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Veröffentlicht in:PloS one 2022-02, Vol.17 (2), p.e0263822-e0263822
Hauptverfasser: Power, Erica A, Fernandez-Torres, Jenelys, Zhang, Liang, Yaun, Ruiyi, Lucien, Fabrice, Daniels, David J
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Sprache:eng
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Zusammenfassung:Diffuse intrinsic pontine glioma (DIPG) is a lethal pediatric brain tumor. While there are a number of in vivo rodent models for evaluating tumor biology and response to therapy, these models require significant time and resources. Here, we established the chick-embryo chorioallantoic (CAM) assay as an affordable and time efficient xenograft model for testing a variety of treatment approaches for DIPG. We found that patient-derived DIPG tumors develop in the CAM and maintain the same genetic and epigenetic characteristics of native DIPG tumors. We monitored tumor response to pharmaco- and radiation therapy by 3-D ultrasound volumetric and vasculature analysis. In this study, we established and validated the CAM model as a potential intermediate xenograft model for DIPG and its use for testing novel treatment approaches that include pharmacotherapy or radiation.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0263822