Evaluation of a parasite-density based pooled targeted amplicon deep sequencing (TADS) method for molecular surveillance of Plasmodium falciparum drug resistance genes in Haiti

Sequencing large numbers of individual samples is often needed for countrywide antimalarial drug resistance surveillance. Pooling DNA from several individual samples is an alternative cost and time saving approach for providing allele frequency (AF) estimates at a population level. Using 100 individ...

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Veröffentlicht in:	PloS one 2022-01, Vol.17 (1), p.e0262616
Hauptverfasser:	Louha, Swarnali, Herman, Camelia, Gupta, Mansi, Patel, Dhruviben, Kelley, Julia, Oh, Je-Hoon M, Guru, Janani, Lemoine, Jean F, Chang, Michelle A, Venkatachalam, Udhayakumar, Rogier, Eric, Talundzic, Eldin
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antimalarials - pharmacology Biology and Life Sciences Blood Causes of Chloroquine Density Deoxyribonucleic acid Diagnostic tests Dihydrofolate reductase Discordance Disease control Disease prevention Disease transmission DNA DNA sequencing Dried Blood Spot Testing - methods Drug resistance Drug Resistance - genetics Drug therapy Epidemiological Monitoring Estimates Evaluation Gene frequency Gene sequencing Genes Genetic aspects Genetic testing Genotyping Haiti Health aspects Health facilities Health surveillance High-Throughput Nucleotide Sequencing - methods High-Throughput Screening Assays - methods Human subjects Humans Malaria Malaria - epidemiology Malaria, Falciparum - parasitology MDR1 protein Medicine and Health Sciences Methods Mutation Nucleic Acid Amplification Techniques - methods Nucleotide sequencing P-Glycoprotein Parasite resistance Parasites Parasites - genetics Parasitic diseases Patients People and places Plasmodium falciparum Plasmodium falciparum - drug effects Plasmodium falciparum - genetics Plasmodium falciparum - pathogenicity Polymerase chain reaction Polymerase Chain Reaction - methods Pools Population genetics Population studies Population-based studies Research and analysis methods Sentinel health events Sequence Analysis, DNA Vector-borne diseases
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