Correlation of breast cancer microcirculation construction with tumor stem cells (CSCs) and epithelial-mesenchymal transition (EMT) based on contrast-enhanced ultrasound (CEUS)
This study is to explore the correlation between the contrast-enhanced ultrasound (CEUS) characteristics of breast cancer and the epithelial-mesenchyme transformation (EMT). Totally 119 patients of breast cancer underwent CEUS. Tissues in the active area were collected and subjected to the immunohis...
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| Veröffentlicht in: | PloS one 2021-12, Vol.16 (12), p.e0261138-e0261138 |
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| creator | Leng, Xiaoling Huang, Guofu Li, Siyi Yao, Miaomiao Ding, Jianbing Ma, Fucheng |
| description | This study is to explore the correlation between the contrast-enhanced ultrasound (CEUS) characteristics of breast cancer and the epithelial-mesenchyme transformation (EMT).
Totally 119 patients of breast cancer underwent CEUS. Tissues in the active area were collected and subjected to the immunohistochemical detection, PT-PCR and Western blot. Correlation analysis was conducted between the clinical pathological parameters and the CEUS indicators.
The expression levels of CD44, N-cadherin, and β-catenin in breast cancer tissues were higher than those in adjacent tissues (P |
| doi_str_mv | 10.1371/journal.pone.0261138 |
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| fullrecord | <record><control><sourceid>proquest_plos_</sourceid><recordid>TN_cdi_plos_journals_2612381914</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_9cb9b90317674066b0fa7315b1746edb</doaj_id><sourcerecordid>2612734556</sourcerecordid><originalsourceid>FETCH-LOGICAL-c526t-a2c4d7ae1043cf867ceb614469c6bd490c72602b4b6b9f7dde4b96bb5d0d7d963</originalsourceid><addsrcrecordid>eNptkttu1DAQhiMEogd4AwSWuNm9yGLHjr2-QULRApWKuGh7bfkw6WblxIudgPpWPGKT3bRqEVc-zD-fZ8Z_lr0jeEWoIJ92YYid9qt96GCFC04IXb_ITomkRc4LTF8-2Z9kZyntMC7pmvPX2QllY6SU4jT7W4UYweu-CR0KNTIRdOqR1Z2FiNrGxmCbaIdZYUOX-jjYw-FP029RP7QhotRDiyx4n9CiuqrSEunOIdiPCvCN9nkLCTq7vWu1R33UXWoOiMXmx_USGZ3AoSN-DKY-h247VeDQ4KeLMIy0RbW5uVq-yV7V2id4O6_n2c3XzXX1Pb_8-e2i-nKZ27Lgfa4Ly5zQQDCjtl5zYcFwwhiXlhvHJLai4LgwzHAja-EcMCO5MaXDTjjJ6Xn24cjd-5DUPOykxjkXdE0kYaPi4qhwQe_UPjatjncq6EYdLkK8VTr2jfWgpDXSSEyJ4IJhzg2utaCkNEQwDs6MrM_za4NpwVmY5uCfQZ9HumarbsNvteaS8LIcAYsZEMOvAVKv2iZNH6I7CMOxbkFZWU6dffxH-v_u2FE1OiClCPVjMQSryYAPWWoyoJoNOKa9f9rIY9KD4-g9jXbdCA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2612381914</pqid></control><display><type>article</type><title>Correlation of breast cancer microcirculation construction with tumor stem cells (CSCs) and epithelial-mesenchymal transition (EMT) based on contrast-enhanced ultrasound (CEUS)</title><source>Public Library of Science (PLoS) Journals Open Access</source><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Leng, Xiaoling ; Huang, Guofu ; Li, Siyi ; Yao, Miaomiao ; Ding, Jianbing ; Ma, Fucheng</creator><contributor>Tsirka, Stella E.</contributor><creatorcontrib>Leng, Xiaoling ; Huang, Guofu ; Li, Siyi ; Yao, Miaomiao ; Ding, Jianbing ; Ma, Fucheng ; Tsirka, Stella E.</creatorcontrib><description>This study is to explore the correlation between the contrast-enhanced ultrasound (CEUS) characteristics of breast cancer and the epithelial-mesenchyme transformation (EMT).
Totally 119 patients of breast cancer underwent CEUS. Tissues in the active area were collected and subjected to the immunohistochemical detection, PT-PCR and Western blot. Correlation analysis was conducted between the clinical pathological parameters and the CEUS indicators.
The expression levels of CD44, N-cadherin, and β-catenin in breast cancer tissues were higher than those in adjacent tissues (P<0.05). However, the expression levels of CD24 and E-cadherin in breast cancer tissues were lower than those in adjacent tissues (P<0.05). There was no significant difference in E-cadherin mRNA and Vimentin levels between cancer and adjacent tissues (P>0.05). The expressions were up-regulated in the CSCs, with higher histological grade, lymph node metastasis, and negative estrogen receptor (ER) expression. Smaller breast tumors, with no lymph node metastasis, lower clinical stage, and positive ER expression, tended to exhibit the up-regulated epithelial phenotype. Breast tumors, with high histological grade, lymph node metastasis, high clinical staging grade, and negative ER expression, tended to exhibit the up-regulated interstitial phenotype. The peak intensity of the time-intensity curve (TIC) for the CEUS was positively correlated with the CSC marker CD44 and the interstitial phenotype marker N-cadherin. The starting time of enhancement was negatively correlated with the N-cadherin. Area under the curve was positively correlated with the expression of CD44 and N-cadherin, while negatively correlated with the epithelial phenotype marker β-catenin. The time to peak was negatively correlated with the interstitial phenotypes Vimentin and N-cadherin, with no correlation with the E-cadherin or β-catenin.
Breast cancers show the enlarged lesions after enlargement and perfusion defect for the CEUS. The fast-in pattern, high enhancement, and high perfusion in the TIC are correlated with the CSCs and EMT expressions, suggesting poor disease prognosis.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0261138</identifier><identifier>PMID: 34932597</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Biology and Life Sciences ; Biomarkers ; Biomarkers, Tumor - metabolism ; Biopsy ; Breast cancer ; Breast Neoplasms - diagnostic imaging ; Breast Neoplasms - metabolism ; Breast Neoplasms - pathology ; Cancer therapies ; CD44 antigen ; Contrast Media - metabolism ; Correlation ; Correlation analysis ; Cytoplasm ; E-cadherin ; Epithelial-Mesenchymal Transition ; Estrogen receptors ; Estrogens ; Female ; Humans ; Lymph nodes ; Medical prognosis ; Medicine and Health Sciences ; Mesenchyme ; Metastases ; Metastasis ; Microcirculation ; Middle Aged ; Monoclonal antibodies ; mRNA ; N-Cadherin ; Neoplastic Stem Cells - metabolism ; Neoplastic Stem Cells - pathology ; Patients ; Perfusion ; Phenotypes ; Prognosis ; Stem cells ; Tissues ; Tumors ; Ultrasonic imaging ; Ultrasonography, Mammary - methods ; Ultrasound ; Vimentin ; β-Catenin</subject><ispartof>PloS one, 2021-12, Vol.16 (12), p.e0261138-e0261138</ispartof><rights>2021 Leng et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 Leng et al 2021 Leng et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-a2c4d7ae1043cf867ceb614469c6bd490c72602b4b6b9f7dde4b96bb5d0d7d963</citedby><cites>FETCH-LOGICAL-c526t-a2c4d7ae1043cf867ceb614469c6bd490c72602b4b6b9f7dde4b96bb5d0d7d963</cites><orcidid>0000-0001-5506-7665</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8691655/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8691655/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34932597$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Tsirka, Stella E.</contributor><creatorcontrib>Leng, Xiaoling</creatorcontrib><creatorcontrib>Huang, Guofu</creatorcontrib><creatorcontrib>Li, Siyi</creatorcontrib><creatorcontrib>Yao, Miaomiao</creatorcontrib><creatorcontrib>Ding, Jianbing</creatorcontrib><creatorcontrib>Ma, Fucheng</creatorcontrib><title>Correlation of breast cancer microcirculation construction with tumor stem cells (CSCs) and epithelial-mesenchymal transition (EMT) based on contrast-enhanced ultrasound (CEUS)</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>This study is to explore the correlation between the contrast-enhanced ultrasound (CEUS) characteristics of breast cancer and the epithelial-mesenchyme transformation (EMT).
Totally 119 patients of breast cancer underwent CEUS. Tissues in the active area were collected and subjected to the immunohistochemical detection, PT-PCR and Western blot. Correlation analysis was conducted between the clinical pathological parameters and the CEUS indicators.
The expression levels of CD44, N-cadherin, and β-catenin in breast cancer tissues were higher than those in adjacent tissues (P<0.05). However, the expression levels of CD24 and E-cadherin in breast cancer tissues were lower than those in adjacent tissues (P<0.05). There was no significant difference in E-cadherin mRNA and Vimentin levels between cancer and adjacent tissues (P>0.05). The expressions were up-regulated in the CSCs, with higher histological grade, lymph node metastasis, and negative estrogen receptor (ER) expression. Smaller breast tumors, with no lymph node metastasis, lower clinical stage, and positive ER expression, tended to exhibit the up-regulated epithelial phenotype. Breast tumors, with high histological grade, lymph node metastasis, high clinical staging grade, and negative ER expression, tended to exhibit the up-regulated interstitial phenotype. The peak intensity of the time-intensity curve (TIC) for the CEUS was positively correlated with the CSC marker CD44 and the interstitial phenotype marker N-cadherin. The starting time of enhancement was negatively correlated with the N-cadherin. Area under the curve was positively correlated with the expression of CD44 and N-cadherin, while negatively correlated with the epithelial phenotype marker β-catenin. The time to peak was negatively correlated with the interstitial phenotypes Vimentin and N-cadherin, with no correlation with the E-cadherin or β-catenin.
Breast cancers show the enlarged lesions after enlargement and perfusion defect for the CEUS. The fast-in pattern, high enhancement, and high perfusion in the TIC are correlated with the CSCs and EMT expressions, suggesting poor disease prognosis.</description><subject>Biology and Life Sciences</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Biopsy</subject><subject>Breast cancer</subject><subject>Breast Neoplasms - diagnostic imaging</subject><subject>Breast Neoplasms - metabolism</subject><subject>Breast Neoplasms - pathology</subject><subject>Cancer therapies</subject><subject>CD44 antigen</subject><subject>Contrast Media - metabolism</subject><subject>Correlation</subject><subject>Correlation analysis</subject><subject>Cytoplasm</subject><subject>E-cadherin</subject><subject>Epithelial-Mesenchymal Transition</subject><subject>Estrogen receptors</subject><subject>Estrogens</subject><subject>Female</subject><subject>Humans</subject><subject>Lymph nodes</subject><subject>Medical prognosis</subject><subject>Medicine and Health Sciences</subject><subject>Mesenchyme</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>Microcirculation</subject><subject>Middle Aged</subject><subject>Monoclonal antibodies</subject><subject>mRNA</subject><subject>N-Cadherin</subject><subject>Neoplastic Stem Cells - metabolism</subject><subject>Neoplastic Stem Cells - pathology</subject><subject>Patients</subject><subject>Perfusion</subject><subject>Phenotypes</subject><subject>Prognosis</subject><subject>Stem cells</subject><subject>Tissues</subject><subject>Tumors</subject><subject>Ultrasonic imaging</subject><subject>Ultrasonography, Mammary - methods</subject><subject>Ultrasound</subject><subject>Vimentin</subject><subject>β-Catenin</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNptkttu1DAQhiMEogd4AwSWuNm9yGLHjr2-QULRApWKuGh7bfkw6WblxIudgPpWPGKT3bRqEVc-zD-fZ8Z_lr0jeEWoIJ92YYid9qt96GCFC04IXb_ITomkRc4LTF8-2Z9kZyntMC7pmvPX2QllY6SU4jT7W4UYweu-CR0KNTIRdOqR1Z2FiNrGxmCbaIdZYUOX-jjYw-FP029RP7QhotRDiyx4n9CiuqrSEunOIdiPCvCN9nkLCTq7vWu1R33UXWoOiMXmx_USGZ3AoSN-DKY-h247VeDQ4KeLMIy0RbW5uVq-yV7V2id4O6_n2c3XzXX1Pb_8-e2i-nKZ27Lgfa4Ly5zQQDCjtl5zYcFwwhiXlhvHJLai4LgwzHAja-EcMCO5MaXDTjjJ6Xn24cjd-5DUPOykxjkXdE0kYaPi4qhwQe_UPjatjncq6EYdLkK8VTr2jfWgpDXSSEyJ4IJhzg2utaCkNEQwDs6MrM_za4NpwVmY5uCfQZ9HumarbsNvteaS8LIcAYsZEMOvAVKv2iZNH6I7CMOxbkFZWU6dffxH-v_u2FE1OiClCPVjMQSryYAPWWoyoJoNOKa9f9rIY9KD4-g9jXbdCA</recordid><startdate>20211221</startdate><enddate>20211221</enddate><creator>Leng, Xiaoling</creator><creator>Huang, Guofu</creator><creator>Li, Siyi</creator><creator>Yao, Miaomiao</creator><creator>Ding, Jianbing</creator><creator>Ma, Fucheng</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-5506-7665</orcidid></search><sort><creationdate>20211221</creationdate><title>Correlation of breast cancer microcirculation construction with tumor stem cells (CSCs) and epithelial-mesenchymal transition (EMT) based on contrast-enhanced ultrasound (CEUS)</title><author>Leng, Xiaoling ; Huang, Guofu ; Li, Siyi ; Yao, Miaomiao ; Ding, Jianbing ; Ma, Fucheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-a2c4d7ae1043cf867ceb614469c6bd490c72602b4b6b9f7dde4b96bb5d0d7d963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biology and Life Sciences</topic><topic>Biomarkers</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Biopsy</topic><topic>Breast cancer</topic><topic>Breast Neoplasms - diagnostic imaging</topic><topic>Breast Neoplasms - metabolism</topic><topic>Breast Neoplasms - pathology</topic><topic>Cancer therapies</topic><topic>CD44 antigen</topic><topic>Contrast Media - metabolism</topic><topic>Correlation</topic><topic>Correlation analysis</topic><topic>Cytoplasm</topic><topic>E-cadherin</topic><topic>Epithelial-Mesenchymal Transition</topic><topic>Estrogen receptors</topic><topic>Estrogens</topic><topic>Female</topic><topic>Humans</topic><topic>Lymph nodes</topic><topic>Medical prognosis</topic><topic>Medicine and Health Sciences</topic><topic>Mesenchyme</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>Microcirculation</topic><topic>Middle Aged</topic><topic>Monoclonal antibodies</topic><topic>mRNA</topic><topic>N-Cadherin</topic><topic>Neoplastic Stem Cells - metabolism</topic><topic>Neoplastic Stem Cells - pathology</topic><topic>Patients</topic><topic>Perfusion</topic><topic>Phenotypes</topic><topic>Prognosis</topic><topic>Stem cells</topic><topic>Tissues</topic><topic>Tumors</topic><topic>Ultrasonic imaging</topic><topic>Ultrasonography, Mammary - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leng, Xiaoling</au><au>Huang, Guofu</au><au>Li, Siyi</au><au>Yao, Miaomiao</au><au>Ding, Jianbing</au><au>Ma, Fucheng</au><au>Tsirka, Stella E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Correlation of breast cancer microcirculation construction with tumor stem cells (CSCs) and epithelial-mesenchymal transition (EMT) based on contrast-enhanced ultrasound (CEUS)</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2021-12-21</date><risdate>2021</risdate><volume>16</volume><issue>12</issue><spage>e0261138</spage><epage>e0261138</epage><pages>e0261138-e0261138</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>This study is to explore the correlation between the contrast-enhanced ultrasound (CEUS) characteristics of breast cancer and the epithelial-mesenchyme transformation (EMT).
Totally 119 patients of breast cancer underwent CEUS. Tissues in the active area were collected and subjected to the immunohistochemical detection, PT-PCR and Western blot. Correlation analysis was conducted between the clinical pathological parameters and the CEUS indicators.
The expression levels of CD44, N-cadherin, and β-catenin in breast cancer tissues were higher than those in adjacent tissues (P<0.05). However, the expression levels of CD24 and E-cadherin in breast cancer tissues were lower than those in adjacent tissues (P<0.05). There was no significant difference in E-cadherin mRNA and Vimentin levels between cancer and adjacent tissues (P>0.05). The expressions were up-regulated in the CSCs, with higher histological grade, lymph node metastasis, and negative estrogen receptor (ER) expression. Smaller breast tumors, with no lymph node metastasis, lower clinical stage, and positive ER expression, tended to exhibit the up-regulated epithelial phenotype. Breast tumors, with high histological grade, lymph node metastasis, high clinical staging grade, and negative ER expression, tended to exhibit the up-regulated interstitial phenotype. The peak intensity of the time-intensity curve (TIC) for the CEUS was positively correlated with the CSC marker CD44 and the interstitial phenotype marker N-cadherin. The starting time of enhancement was negatively correlated with the N-cadherin. Area under the curve was positively correlated with the expression of CD44 and N-cadherin, while negatively correlated with the epithelial phenotype marker β-catenin. The time to peak was negatively correlated with the interstitial phenotypes Vimentin and N-cadherin, with no correlation with the E-cadherin or β-catenin.
Breast cancers show the enlarged lesions after enlargement and perfusion defect for the CEUS. The fast-in pattern, high enhancement, and high perfusion in the TIC are correlated with the CSCs and EMT expressions, suggesting poor disease prognosis.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>34932597</pmid><doi>10.1371/journal.pone.0261138</doi><orcidid>https://orcid.org/0000-0001-5506-7665</orcidid><oa>free_for_read</oa></addata></record> |
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| issn | 1932-6203 1932-6203 |
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| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Biology and Life Sciences Biomarkers Biomarkers, Tumor - metabolism Biopsy Breast cancer Breast Neoplasms - diagnostic imaging Breast Neoplasms - metabolism Breast Neoplasms - pathology Cancer therapies CD44 antigen Contrast Media - metabolism Correlation Correlation analysis Cytoplasm E-cadherin Epithelial-Mesenchymal Transition Estrogen receptors Estrogens Female Humans Lymph nodes Medical prognosis Medicine and Health Sciences Mesenchyme Metastases Metastasis Microcirculation Middle Aged Monoclonal antibodies mRNA N-Cadherin Neoplastic Stem Cells - metabolism Neoplastic Stem Cells - pathology Patients Perfusion Phenotypes Prognosis Stem cells Tissues Tumors Ultrasonic imaging Ultrasonography, Mammary - methods Ultrasound Vimentin β-Catenin |
| title | Correlation of breast cancer microcirculation construction with tumor stem cells (CSCs) and epithelial-mesenchymal transition (EMT) based on contrast-enhanced ultrasound (CEUS) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T20%3A01%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Correlation%20of%20breast%20cancer%20microcirculation%20construction%20with%20tumor%20stem%20cells%20(CSCs)%20and%20epithelial-mesenchymal%20transition%20(EMT)%20based%20on%20contrast-enhanced%20ultrasound%20(CEUS)&rft.jtitle=PloS%20one&rft.au=Leng,%20Xiaoling&rft.date=2021-12-21&rft.volume=16&rft.issue=12&rft.spage=e0261138&rft.epage=e0261138&rft.pages=e0261138-e0261138&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0261138&rft_dat=%3Cproquest_plos_%3E2612734556%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2612381914&rft_id=info:pmid/34932597&rft_doaj_id=oai_doaj_org_article_9cb9b90317674066b0fa7315b1746edb&rfr_iscdi=true |