Larazotide acetate induces recovery of ischemia-injured porcine jejunum via repair of tight junctions

Larazotide acetate induces recovery of ischemia-injured porcine jejunum via repair of tight junctions

Intestinal ischemia results in mucosal injury, including paracellular barrier loss due to disruption of tight junctions. Larazotide acetate (LA), a small peptide studied in Phase III clinical trials for treatment of celiac disease, regulates tight junctions (TJs). We hypothesized that LA would dose-...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:PloS one 2021-04, Vol.16 (4), p.e0250165-e0250165
Hauptverfasser: Slifer, Zachary M, Hernandez, Liliana, Pridgen, Tiffany A, Carlson, Alexandra R, Messenger, Kristen M, Madan, Jay, Krishnan, B Radha, Laumas, Sandeep, Blikslager, Anthony T
Format: Artikel
Sprache:eng
Schlagworte:
Acetates
Acetic acid
Animals
Biology and Life Sciences
Biotechnology
Clinical trials
Data analysis
Disease Models, Animal
Drug dosages
Drug therapy
E coli
Editing
Epithelium
Female
Funding
Injuries
Intestinal ischemia
Intestinal Mucosa - drug effects
Intestinal Mucosa - metabolism
Intestine
Ischemia
Ischemia - drug therapy
Ischemia - metabolism
Jejunum
Jejunum - blood supply
Jejunum - drug effects
Jejunum - metabolism
Male
Medical research
Medicine
Medicine and Health Sciences
Methodology
Mucosa
Oligopeptides - pharmacology
Oligopeptides - therapeutic use
Permeability
Permeability - drug effects
Pharmacology
Physiological aspects
Proteins
Recovery
Research and Analysis Methods
Reviews
Small intestine
Standard deviation
Swine
Testing
Tight junctions
Tight Junctions - drug effects
Tight Junctions - metabolism
Toxins
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Intestinal ischemia results in mucosal injury, including paracellular barrier loss due to disruption of tight junctions. Larazotide acetate (LA), a small peptide studied in Phase III clinical trials for treatment of celiac disease, regulates tight junctions (TJs). We hypothesized that LA would dose-dependently hasten recovery of intestinal ischemic injury via modulation of TJs. Ischemia-injured tissue from 6-8-week-old pigs was recovered in Ussing chambers for 240-minutes in the presence of LA. LA (1 μM but not 0.1 μM or 10 μM) significantly enhanced transepithelial electrical resistance (TER) above ischemic injured controls and significantly reduced serosal-to-mucosal flux LPS (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0250165