Diagnostic accuracy of antigen detection in urine and molecular assays testing in different clinical samples for the diagnosis of progressive disseminated histoplasmosis in patients living with HIV/AIDS: A prospective multicenter study in Mexico
The progressive disseminated histoplasmosis (PDH) has been associated with severe disease and high risk of death among people living with HIV (PLWHIV). Therefore, the purpose of this multicenter, prospective, double-blinded study done in ten Mexican hospitals was to determine the diagnostic accuracy...
Gespeichert in:
| Veröffentlicht in: | PLoS neglected tropical diseases 2021-03, Vol.15 (3), p.e0009215-e0009215 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e0009215 |
|---|---|
| container_issue | 3 |
| container_start_page | e0009215 |
| container_title | PLoS neglected tropical diseases |
| container_volume | 15 |
| creator | Martínez-Gamboa, Areli Niembro-Ortega, María Dolores Torres-González, Pedro Santiago-Cruz, Janeth Velázquez-Zavala, Nancy Guadalupe Rangel-Cordero, Andrea Crabtree-Ramírez, Brenda Gamboa-Domínguez, Armando Reyes-Gutiérrez, Edgardo Reyes-Terán, Gustavo Lozano-Fernandez, Víctor Hugo Ahumada-Topete, Víctor Hugo Martínez-Ayala, Pedro Manríquez-Reyes, Marisol Ramírez-Hinojosa, Juan Pablo Rodríguez-Zulueta, Patricia Hernández-León, Christian Ruíz-Quiñones, Jesús Rivera-Martínez, Norma Eréndira Chaparro-Sánchez, Alberto Andrade-Villanueva, Jaime González-Hernández, Luz Alicia Cruz-Martínez, Sofia Flores-Barrientos, Oscar Gaytán-Martínez, Jesús Enrique Magaña-Aquino, Martín Cervantes-Sánchez, Axel Olivas-Martínez, Antonio Araujo-Meléndez, Javier Del Rocío Reyes-Montes, María Duarte-Escalante, Esperanza Frías-De León, María Guadalupe Ramírez, José Antonio Taylor, María Lucia de León-Garduño, Alfredo Ponce Sifuentes-Osornio, José |
| description | The progressive disseminated histoplasmosis (PDH) has been associated with severe disease and high risk of death among people living with HIV (PLWHIV). Therefore, the purpose of this multicenter, prospective, double-blinded study done in ten Mexican hospitals was to determine the diagnostic accuracy of detecting Histoplasma capsulatum antigen in urine using the IMMY ALPHA Histoplasma EIA kit (IAHE), clarus Histoplasma GM Enzyme Immunoassay (cHGEI IMMY) and MiraVista Histoplasma Urine Antigen LFA (MVHUALFA); as well as the Hcp100 and 1281-1283220SCAR nested PCRs in blood, bone-marrow, tissue biopsies and urine.
We included 415 PLWHIV older than 18 years of age with suspicion of PDH. Using as diagnostic standard recovery of H. capsulatum in blood, bone marrow or tissue cultures, or histopathological exam compatible, detected 108 patients (26%, [95%CI, 21.78-30.22]) with proven-PDH. We analyzed 391 urine samples by the IAHE, cHGEI IMMY and MVHUALFA; the sensitivity/specificity values obtained were 67.3% (95% CI, 57.4-76.2) / 96.2% (95% CI, 93.2-98.0) for IAHE, 91.3% (95% CI, 84.2-96.0) / 90.9% (95% CI, 87.0-94.0) for cHGEI IMMY and 90.4% (95% CI, 83.0-95.3) / 92.3% (95% CI, 88.6-95.1) for MVHUALFA. The Hcp100 nested PCR was performed on 393, 343, 75 and 297, blood, bone marrow, tissue and urine samples respectively; the sensitivity/specificity values obtained were 62.9% (95%CI, 53.3-72.5)/ 89.5% (95%CI, 86.0-93.0), 65.9% (95%CI, 56.0-75.8)/ 89.0% (95%CI, 85.2-92.9), 62.1% (95%CI, 44.4-79.7)/ 82.6% (95%CI, 71.7-93.6) and 34.9% (95%CI, 24.8-46.2)/ 67.3% (95%CI, 60.6-73.5) respectively; and 1281-1283220SCAR nested PCR was performed on 392, 344, 75 and 291, respectively; the sensitivity/specificity values obtained were 65.3% (95% CI, 55.9-74.7)/ 58.8% (95%CI, 53.2-64.5), 70.8% (95%CI, 61.3-80.2)/ 52.9% (95%CI, 46.8-59.1), 71.4% (95%CI, 54.7-88.2)/ 40.4% (95%CI, 26.4-54.5) and 18.1% (95%CI, 10.5-28.1)/ 90.4% (95%CI, 85.5-94.0), respectively.
The cHGEI IMMY and MVHUALFA tests showed excellent performance for the diagnosis of PDH in PLWHIV. The integration of these tests in clinical laboratories will certainly impact on early diagnosis and treatment. |
| doi_str_mv | 10.1371/journal.pntd.0009215 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_2513691730</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A658713898</galeid><doaj_id>oai_doaj_org_article_b93579871f1a49d1a1f7166df3e2a1d2</doaj_id><sourcerecordid>A658713898</sourcerecordid><originalsourceid>FETCH-LOGICAL-c624t-d3c053c0936cc324a277a8979a6742f1f79e6230982544d68d590f2cc07f1ae03</originalsourceid><addsrcrecordid>eNptk01v1DAQhiMEoqXwDxBYQkJcdhvH-TIHpFULdKUiDnxcrantZF05drCdwv5w7kzabdVFVRQlsp95Z157Jste0nxJWUOPL_0UHNjl6JJa5nnOC1o9yg4pZ9WiaFj1-N7_QfYsxss8r3jV0qfZAWN1W9KiPcz-nhronY_JSAJSTgHklviOgEum144onbRMxjtiHJmCcRq3FBm81XKyEAjECNtIkkYJ18-UMl2ng3aJSGuckWBJhGG0OpLOB5I2GpHrpCbOqcbg-6BjNFfzRox6MA6SVmRjYvKjhThcoyg9QjIoHIk1V3O23yZtyNn65_FqffrtPVnNWnGcC0atYbLoCnEdSEyT2s4KX_QfI_3z7EkHNuoXu-9R9uPTx-8nZ4vzr5_XJ6vzhayLMi0Uk3mFL2e1lKwooWgaaHnDoW7KoqNdw3VdsJy3RVWWqm5VxfOukDJvOgo6Z0fZ6xvd0foodjcWRVFRVnPasJlY3xDKw6UYgxkgbIUHI64XfOgFBLRhtbjA22x421DULrmigPlpXauO6QKoKlDrwy7bdDFoNVsPYPdE93ec2YjeX4mGNxRtocC7nUDwvya8UTGYKLW14LSfsO6Sc8ax10pE3_yHPuxuR_WABozrPOaVs6hY1RVaYS1vkVo-QOGjsBWkd7ozuL4X8PZewEaDTZvo7TT3adwHyxtQYl_EoLu7w6C5mKfotmoxT5HYTRGGvbp_kHdBt2PD_gEkMB9K</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2513691730</pqid></control><display><type>article</type><title>Diagnostic accuracy of antigen detection in urine and molecular assays testing in different clinical samples for the diagnosis of progressive disseminated histoplasmosis in patients living with HIV/AIDS: A prospective multicenter study in Mexico</title><source>DOAJ Directory of Open Access Journals</source><source>PubMed Central Open Access</source><source>Public Library of Science (PLoS)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Martínez-Gamboa, Areli ; Niembro-Ortega, María Dolores ; Torres-González, Pedro ; Santiago-Cruz, Janeth ; Velázquez-Zavala, Nancy Guadalupe ; Rangel-Cordero, Andrea ; Crabtree-Ramírez, Brenda ; Gamboa-Domínguez, Armando ; Reyes-Gutiérrez, Edgardo ; Reyes-Terán, Gustavo ; Lozano-Fernandez, Víctor Hugo ; Ahumada-Topete, Víctor Hugo ; Martínez-Ayala, Pedro ; Manríquez-Reyes, Marisol ; Ramírez-Hinojosa, Juan Pablo ; Rodríguez-Zulueta, Patricia ; Hernández-León, Christian ; Ruíz-Quiñones, Jesús ; Rivera-Martínez, Norma Eréndira ; Chaparro-Sánchez, Alberto ; Andrade-Villanueva, Jaime ; González-Hernández, Luz Alicia ; Cruz-Martínez, Sofia ; Flores-Barrientos, Oscar ; Gaytán-Martínez, Jesús Enrique ; Magaña-Aquino, Martín ; Cervantes-Sánchez, Axel ; Olivas-Martínez, Antonio ; Araujo-Meléndez, Javier ; Del Rocío Reyes-Montes, María ; Duarte-Escalante, Esperanza ; Frías-De León, María Guadalupe ; Ramírez, José Antonio ; Taylor, María Lucia ; de León-Garduño, Alfredo Ponce ; Sifuentes-Osornio, José</creator><contributor>Gonzalez, Angel</contributor><creatorcontrib>Martínez-Gamboa, Areli ; Niembro-Ortega, María Dolores ; Torres-González, Pedro ; Santiago-Cruz, Janeth ; Velázquez-Zavala, Nancy Guadalupe ; Rangel-Cordero, Andrea ; Crabtree-Ramírez, Brenda ; Gamboa-Domínguez, Armando ; Reyes-Gutiérrez, Edgardo ; Reyes-Terán, Gustavo ; Lozano-Fernandez, Víctor Hugo ; Ahumada-Topete, Víctor Hugo ; Martínez-Ayala, Pedro ; Manríquez-Reyes, Marisol ; Ramírez-Hinojosa, Juan Pablo ; Rodríguez-Zulueta, Patricia ; Hernández-León, Christian ; Ruíz-Quiñones, Jesús ; Rivera-Martínez, Norma Eréndira ; Chaparro-Sánchez, Alberto ; Andrade-Villanueva, Jaime ; González-Hernández, Luz Alicia ; Cruz-Martínez, Sofia ; Flores-Barrientos, Oscar ; Gaytán-Martínez, Jesús Enrique ; Magaña-Aquino, Martín ; Cervantes-Sánchez, Axel ; Olivas-Martínez, Antonio ; Araujo-Meléndez, Javier ; Del Rocío Reyes-Montes, María ; Duarte-Escalante, Esperanza ; Frías-De León, María Guadalupe ; Ramírez, José Antonio ; Taylor, María Lucia ; de León-Garduño, Alfredo Ponce ; Sifuentes-Osornio, José ; Gonzalez, Angel</creatorcontrib><description>The progressive disseminated histoplasmosis (PDH) has been associated with severe disease and high risk of death among people living with HIV (PLWHIV). Therefore, the purpose of this multicenter, prospective, double-blinded study done in ten Mexican hospitals was to determine the diagnostic accuracy of detecting Histoplasma capsulatum antigen in urine using the IMMY ALPHA Histoplasma EIA kit (IAHE), clarus Histoplasma GM Enzyme Immunoassay (cHGEI IMMY) and MiraVista Histoplasma Urine Antigen LFA (MVHUALFA); as well as the Hcp100 and 1281-1283220SCAR nested PCRs in blood, bone-marrow, tissue biopsies and urine.
We included 415 PLWHIV older than 18 years of age with suspicion of PDH. Using as diagnostic standard recovery of H. capsulatum in blood, bone marrow or tissue cultures, or histopathological exam compatible, detected 108 patients (26%, [95%CI, 21.78-30.22]) with proven-PDH. We analyzed 391 urine samples by the IAHE, cHGEI IMMY and MVHUALFA; the sensitivity/specificity values obtained were 67.3% (95% CI, 57.4-76.2) / 96.2% (95% CI, 93.2-98.0) for IAHE, 91.3% (95% CI, 84.2-96.0) / 90.9% (95% CI, 87.0-94.0) for cHGEI IMMY and 90.4% (95% CI, 83.0-95.3) / 92.3% (95% CI, 88.6-95.1) for MVHUALFA. The Hcp100 nested PCR was performed on 393, 343, 75 and 297, blood, bone marrow, tissue and urine samples respectively; the sensitivity/specificity values obtained were 62.9% (95%CI, 53.3-72.5)/ 89.5% (95%CI, 86.0-93.0), 65.9% (95%CI, 56.0-75.8)/ 89.0% (95%CI, 85.2-92.9), 62.1% (95%CI, 44.4-79.7)/ 82.6% (95%CI, 71.7-93.6) and 34.9% (95%CI, 24.8-46.2)/ 67.3% (95%CI, 60.6-73.5) respectively; and 1281-1283220SCAR nested PCR was performed on 392, 344, 75 and 291, respectively; the sensitivity/specificity values obtained were 65.3% (95% CI, 55.9-74.7)/ 58.8% (95%CI, 53.2-64.5), 70.8% (95%CI, 61.3-80.2)/ 52.9% (95%CI, 46.8-59.1), 71.4% (95%CI, 54.7-88.2)/ 40.4% (95%CI, 26.4-54.5) and 18.1% (95%CI, 10.5-28.1)/ 90.4% (95%CI, 85.5-94.0), respectively.
The cHGEI IMMY and MVHUALFA tests showed excellent performance for the diagnosis of PDH in PLWHIV. The integration of these tests in clinical laboratories will certainly impact on early diagnosis and treatment.</description><identifier>ISSN: 1935-2735</identifier><identifier>ISSN: 1935-2727</identifier><identifier>EISSN: 1935-2735</identifier><identifier>DOI: 10.1371/journal.pntd.0009215</identifier><identifier>PMID: 33684128</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Accuracy ; Acquired immune deficiency syndrome ; AIDS ; Analysis ; Antigens ; Aspartate aminotransferase ; Biology and Life Sciences ; Biopsy ; Bleeding ; Body weight ; Body weight loss ; Bone marrow ; Care and treatment ; Cholecystitis ; Consortia ; Deoxyribonucleic acid ; Diagnosis ; Diagnostic systems ; Diarrhea ; Disease ; DNA ; Enzyme immunoassay ; Enzymes ; Epididymitis ; Ethical standards ; Ethics ; Ferritin ; Fever ; Fungal infections ; Fungi ; Heart ; Hemoglobin ; Histopathology ; Histoplasmosis ; HIV ; HIV patients ; Human immunodeficiency virus ; Immunoassay ; Immunoassays ; Laboratories ; Lesions ; Leukocytes (neutrophilic) ; Lymphadenopathy ; Markers ; Medical testing products ; Medicine and Health Sciences ; Mycosis ; Observers ; Pancreatitis ; Pancytopenia ; Patients ; Physiological aspects ; Platelets ; Polymerase chain reaction ; Prostate ; Protocols ; Research and Analysis Methods ; Skin diseases ; Spleen ; Splenomegaly ; Testing ; Tropical diseases ; Tuberculosis ; Ulcers ; Urine ; Weight loss ; Yeasts</subject><ispartof>PLoS neglected tropical diseases, 2021-03, Vol.15 (3), p.e0009215-e0009215</ispartof><rights>COPYRIGHT 2021 Public Library of Science</rights><rights>2021 Martínez-Gamboa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2021 Martínez-Gamboa et al 2021 Martínez-Gamboa et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c624t-d3c053c0936cc324a277a8979a6742f1f79e6230982544d68d590f2cc07f1ae03</citedby><cites>FETCH-LOGICAL-c624t-d3c053c0936cc324a277a8979a6742f1f79e6230982544d68d590f2cc07f1ae03</cites><orcidid>0000-0002-0905-0835 ; 0000-0002-0505-6340 ; 0000-0002-2279-1558 ; 0000-0003-2640-2916 ; 0000-0002-3160-8698 ; 0000-0001-5395-6418 ; 0000-0003-2613-4878 ; 0000-0003-1948-655X ; 0000-0001-5094-7890 ; 0000-0002-4609-4415 ; 0000-0002-1983-0384 ; 0000-0001-9822-3496 ; 0000-0002-7163-0566 ; 0000-0002-6315-7860 ; 0000-0003-2779-0068 ; 0000-0001-9584-1360 ; 0000-0002-6716-1234 ; 0000-0002-3866-9995 ; 0000-0001-5011-5939 ; 0000-0002-2587-1123 ; 0000-0002-3773-613X ; 0000-0002-9545-2522 ; 0000-0002-3723-333X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971897/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971897/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2926,23865,27923,27924,53790,53792,79371,79372</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33684128$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Gonzalez, Angel</contributor><creatorcontrib>Martínez-Gamboa, Areli</creatorcontrib><creatorcontrib>Niembro-Ortega, María Dolores</creatorcontrib><creatorcontrib>Torres-González, Pedro</creatorcontrib><creatorcontrib>Santiago-Cruz, Janeth</creatorcontrib><creatorcontrib>Velázquez-Zavala, Nancy Guadalupe</creatorcontrib><creatorcontrib>Rangel-Cordero, Andrea</creatorcontrib><creatorcontrib>Crabtree-Ramírez, Brenda</creatorcontrib><creatorcontrib>Gamboa-Domínguez, Armando</creatorcontrib><creatorcontrib>Reyes-Gutiérrez, Edgardo</creatorcontrib><creatorcontrib>Reyes-Terán, Gustavo</creatorcontrib><creatorcontrib>Lozano-Fernandez, Víctor Hugo</creatorcontrib><creatorcontrib>Ahumada-Topete, Víctor Hugo</creatorcontrib><creatorcontrib>Martínez-Ayala, Pedro</creatorcontrib><creatorcontrib>Manríquez-Reyes, Marisol</creatorcontrib><creatorcontrib>Ramírez-Hinojosa, Juan Pablo</creatorcontrib><creatorcontrib>Rodríguez-Zulueta, Patricia</creatorcontrib><creatorcontrib>Hernández-León, Christian</creatorcontrib><creatorcontrib>Ruíz-Quiñones, Jesús</creatorcontrib><creatorcontrib>Rivera-Martínez, Norma Eréndira</creatorcontrib><creatorcontrib>Chaparro-Sánchez, Alberto</creatorcontrib><creatorcontrib>Andrade-Villanueva, Jaime</creatorcontrib><creatorcontrib>González-Hernández, Luz Alicia</creatorcontrib><creatorcontrib>Cruz-Martínez, Sofia</creatorcontrib><creatorcontrib>Flores-Barrientos, Oscar</creatorcontrib><creatorcontrib>Gaytán-Martínez, Jesús Enrique</creatorcontrib><creatorcontrib>Magaña-Aquino, Martín</creatorcontrib><creatorcontrib>Cervantes-Sánchez, Axel</creatorcontrib><creatorcontrib>Olivas-Martínez, Antonio</creatorcontrib><creatorcontrib>Araujo-Meléndez, Javier</creatorcontrib><creatorcontrib>Del Rocío Reyes-Montes, María</creatorcontrib><creatorcontrib>Duarte-Escalante, Esperanza</creatorcontrib><creatorcontrib>Frías-De León, María Guadalupe</creatorcontrib><creatorcontrib>Ramírez, José Antonio</creatorcontrib><creatorcontrib>Taylor, María Lucia</creatorcontrib><creatorcontrib>de León-Garduño, Alfredo Ponce</creatorcontrib><creatorcontrib>Sifuentes-Osornio, José</creatorcontrib><title>Diagnostic accuracy of antigen detection in urine and molecular assays testing in different clinical samples for the diagnosis of progressive disseminated histoplasmosis in patients living with HIV/AIDS: A prospective multicenter study in Mexico</title><title>PLoS neglected tropical diseases</title><addtitle>PLoS Negl Trop Dis</addtitle><description>The progressive disseminated histoplasmosis (PDH) has been associated with severe disease and high risk of death among people living with HIV (PLWHIV). Therefore, the purpose of this multicenter, prospective, double-blinded study done in ten Mexican hospitals was to determine the diagnostic accuracy of detecting Histoplasma capsulatum antigen in urine using the IMMY ALPHA Histoplasma EIA kit (IAHE), clarus Histoplasma GM Enzyme Immunoassay (cHGEI IMMY) and MiraVista Histoplasma Urine Antigen LFA (MVHUALFA); as well as the Hcp100 and 1281-1283220SCAR nested PCRs in blood, bone-marrow, tissue biopsies and urine.
We included 415 PLWHIV older than 18 years of age with suspicion of PDH. Using as diagnostic standard recovery of H. capsulatum in blood, bone marrow or tissue cultures, or histopathological exam compatible, detected 108 patients (26%, [95%CI, 21.78-30.22]) with proven-PDH. We analyzed 391 urine samples by the IAHE, cHGEI IMMY and MVHUALFA; the sensitivity/specificity values obtained were 67.3% (95% CI, 57.4-76.2) / 96.2% (95% CI, 93.2-98.0) for IAHE, 91.3% (95% CI, 84.2-96.0) / 90.9% (95% CI, 87.0-94.0) for cHGEI IMMY and 90.4% (95% CI, 83.0-95.3) / 92.3% (95% CI, 88.6-95.1) for MVHUALFA. The Hcp100 nested PCR was performed on 393, 343, 75 and 297, blood, bone marrow, tissue and urine samples respectively; the sensitivity/specificity values obtained were 62.9% (95%CI, 53.3-72.5)/ 89.5% (95%CI, 86.0-93.0), 65.9% (95%CI, 56.0-75.8)/ 89.0% (95%CI, 85.2-92.9), 62.1% (95%CI, 44.4-79.7)/ 82.6% (95%CI, 71.7-93.6) and 34.9% (95%CI, 24.8-46.2)/ 67.3% (95%CI, 60.6-73.5) respectively; and 1281-1283220SCAR nested PCR was performed on 392, 344, 75 and 291, respectively; the sensitivity/specificity values obtained were 65.3% (95% CI, 55.9-74.7)/ 58.8% (95%CI, 53.2-64.5), 70.8% (95%CI, 61.3-80.2)/ 52.9% (95%CI, 46.8-59.1), 71.4% (95%CI, 54.7-88.2)/ 40.4% (95%CI, 26.4-54.5) and 18.1% (95%CI, 10.5-28.1)/ 90.4% (95%CI, 85.5-94.0), respectively.
The cHGEI IMMY and MVHUALFA tests showed excellent performance for the diagnosis of PDH in PLWHIV. The integration of these tests in clinical laboratories will certainly impact on early diagnosis and treatment.</description><subject>Accuracy</subject><subject>Acquired immune deficiency syndrome</subject><subject>AIDS</subject><subject>Analysis</subject><subject>Antigens</subject><subject>Aspartate aminotransferase</subject><subject>Biology and Life Sciences</subject><subject>Biopsy</subject><subject>Bleeding</subject><subject>Body weight</subject><subject>Body weight loss</subject><subject>Bone marrow</subject><subject>Care and treatment</subject><subject>Cholecystitis</subject><subject>Consortia</subject><subject>Deoxyribonucleic acid</subject><subject>Diagnosis</subject><subject>Diagnostic systems</subject><subject>Diarrhea</subject><subject>Disease</subject><subject>DNA</subject><subject>Enzyme immunoassay</subject><subject>Enzymes</subject><subject>Epididymitis</subject><subject>Ethical standards</subject><subject>Ethics</subject><subject>Ferritin</subject><subject>Fever</subject><subject>Fungal infections</subject><subject>Fungi</subject><subject>Heart</subject><subject>Hemoglobin</subject><subject>Histopathology</subject><subject>Histoplasmosis</subject><subject>HIV</subject><subject>HIV patients</subject><subject>Human immunodeficiency virus</subject><subject>Immunoassay</subject><subject>Immunoassays</subject><subject>Laboratories</subject><subject>Lesions</subject><subject>Leukocytes (neutrophilic)</subject><subject>Lymphadenopathy</subject><subject>Markers</subject><subject>Medical testing products</subject><subject>Medicine and Health Sciences</subject><subject>Mycosis</subject><subject>Observers</subject><subject>Pancreatitis</subject><subject>Pancytopenia</subject><subject>Patients</subject><subject>Physiological aspects</subject><subject>Platelets</subject><subject>Polymerase chain reaction</subject><subject>Prostate</subject><subject>Protocols</subject><subject>Research and Analysis Methods</subject><subject>Skin diseases</subject><subject>Spleen</subject><subject>Splenomegaly</subject><subject>Testing</subject><subject>Tropical diseases</subject><subject>Tuberculosis</subject><subject>Ulcers</subject><subject>Urine</subject><subject>Weight loss</subject><subject>Yeasts</subject><issn>1935-2735</issn><issn>1935-2727</issn><issn>1935-2735</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>DOA</sourceid><recordid>eNptk01v1DAQhiMEoqXwDxBYQkJcdhvH-TIHpFULdKUiDnxcrantZF05drCdwv5w7kzabdVFVRQlsp95Z157Jste0nxJWUOPL_0UHNjl6JJa5nnOC1o9yg4pZ9WiaFj1-N7_QfYsxss8r3jV0qfZAWN1W9KiPcz-nhronY_JSAJSTgHklviOgEum144onbRMxjtiHJmCcRq3FBm81XKyEAjECNtIkkYJ18-UMl2ng3aJSGuckWBJhGG0OpLOB5I2GpHrpCbOqcbg-6BjNFfzRox6MA6SVmRjYvKjhThcoyg9QjIoHIk1V3O23yZtyNn65_FqffrtPVnNWnGcC0atYbLoCnEdSEyT2s4KX_QfI_3z7EkHNuoXu-9R9uPTx-8nZ4vzr5_XJ6vzhayLMi0Uk3mFL2e1lKwooWgaaHnDoW7KoqNdw3VdsJy3RVWWqm5VxfOukDJvOgo6Z0fZ6xvd0foodjcWRVFRVnPasJlY3xDKw6UYgxkgbIUHI64XfOgFBLRhtbjA22x421DULrmigPlpXauO6QKoKlDrwy7bdDFoNVsPYPdE93ec2YjeX4mGNxRtocC7nUDwvya8UTGYKLW14LSfsO6Sc8ax10pE3_yHPuxuR_WABozrPOaVs6hY1RVaYS1vkVo-QOGjsBWkd7ozuL4X8PZewEaDTZvo7TT3adwHyxtQYl_EoLu7w6C5mKfotmoxT5HYTRGGvbp_kHdBt2PD_gEkMB9K</recordid><startdate>20210301</startdate><enddate>20210301</enddate><creator>Martínez-Gamboa, Areli</creator><creator>Niembro-Ortega, María Dolores</creator><creator>Torres-González, Pedro</creator><creator>Santiago-Cruz, Janeth</creator><creator>Velázquez-Zavala, Nancy Guadalupe</creator><creator>Rangel-Cordero, Andrea</creator><creator>Crabtree-Ramírez, Brenda</creator><creator>Gamboa-Domínguez, Armando</creator><creator>Reyes-Gutiérrez, Edgardo</creator><creator>Reyes-Terán, Gustavo</creator><creator>Lozano-Fernandez, Víctor Hugo</creator><creator>Ahumada-Topete, Víctor Hugo</creator><creator>Martínez-Ayala, Pedro</creator><creator>Manríquez-Reyes, Marisol</creator><creator>Ramírez-Hinojosa, Juan Pablo</creator><creator>Rodríguez-Zulueta, Patricia</creator><creator>Hernández-León, Christian</creator><creator>Ruíz-Quiñones, Jesús</creator><creator>Rivera-Martínez, Norma Eréndira</creator><creator>Chaparro-Sánchez, Alberto</creator><creator>Andrade-Villanueva, Jaime</creator><creator>González-Hernández, Luz Alicia</creator><creator>Cruz-Martínez, Sofia</creator><creator>Flores-Barrientos, Oscar</creator><creator>Gaytán-Martínez, Jesús Enrique</creator><creator>Magaña-Aquino, Martín</creator><creator>Cervantes-Sánchez, Axel</creator><creator>Olivas-Martínez, Antonio</creator><creator>Araujo-Meléndez, Javier</creator><creator>Del Rocío Reyes-Montes, María</creator><creator>Duarte-Escalante, Esperanza</creator><creator>Frías-De León, María Guadalupe</creator><creator>Ramírez, José Antonio</creator><creator>Taylor, María Lucia</creator><creator>de León-Garduño, Alfredo Ponce</creator><creator>Sifuentes-Osornio, José</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SS</scope><scope>7T2</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>K9.</scope><scope>L.G</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-0905-0835</orcidid><orcidid>https://orcid.org/0000-0002-0505-6340</orcidid><orcidid>https://orcid.org/0000-0002-2279-1558</orcidid><orcidid>https://orcid.org/0000-0003-2640-2916</orcidid><orcidid>https://orcid.org/0000-0002-3160-8698</orcidid><orcidid>https://orcid.org/0000-0001-5395-6418</orcidid><orcidid>https://orcid.org/0000-0003-2613-4878</orcidid><orcidid>https://orcid.org/0000-0003-1948-655X</orcidid><orcidid>https://orcid.org/0000-0001-5094-7890</orcidid><orcidid>https://orcid.org/0000-0002-4609-4415</orcidid><orcidid>https://orcid.org/0000-0002-1983-0384</orcidid><orcidid>https://orcid.org/0000-0001-9822-3496</orcidid><orcidid>https://orcid.org/0000-0002-7163-0566</orcidid><orcidid>https://orcid.org/0000-0002-6315-7860</orcidid><orcidid>https://orcid.org/0000-0003-2779-0068</orcidid><orcidid>https://orcid.org/0000-0001-9584-1360</orcidid><orcidid>https://orcid.org/0000-0002-6716-1234</orcidid><orcidid>https://orcid.org/0000-0002-3866-9995</orcidid><orcidid>https://orcid.org/0000-0001-5011-5939</orcidid><orcidid>https://orcid.org/0000-0002-2587-1123</orcidid><orcidid>https://orcid.org/0000-0002-3773-613X</orcidid><orcidid>https://orcid.org/0000-0002-9545-2522</orcidid><orcidid>https://orcid.org/0000-0002-3723-333X</orcidid></search><sort><creationdate>20210301</creationdate><title>Diagnostic accuracy of antigen detection in urine and molecular assays testing in different clinical samples for the diagnosis of progressive disseminated histoplasmosis in patients living with HIV/AIDS: A prospective multicenter study in Mexico</title><author>Martínez-Gamboa, Areli ; Niembro-Ortega, María Dolores ; Torres-González, Pedro ; Santiago-Cruz, Janeth ; Velázquez-Zavala, Nancy Guadalupe ; Rangel-Cordero, Andrea ; Crabtree-Ramírez, Brenda ; Gamboa-Domínguez, Armando ; Reyes-Gutiérrez, Edgardo ; Reyes-Terán, Gustavo ; Lozano-Fernandez, Víctor Hugo ; Ahumada-Topete, Víctor Hugo ; Martínez-Ayala, Pedro ; Manríquez-Reyes, Marisol ; Ramírez-Hinojosa, Juan Pablo ; Rodríguez-Zulueta, Patricia ; Hernández-León, Christian ; Ruíz-Quiñones, Jesús ; Rivera-Martínez, Norma Eréndira ; Chaparro-Sánchez, Alberto ; Andrade-Villanueva, Jaime ; González-Hernández, Luz Alicia ; Cruz-Martínez, Sofia ; Flores-Barrientos, Oscar ; Gaytán-Martínez, Jesús Enrique ; Magaña-Aquino, Martín ; Cervantes-Sánchez, Axel ; Olivas-Martínez, Antonio ; Araujo-Meléndez, Javier ; Del Rocío Reyes-Montes, María ; Duarte-Escalante, Esperanza ; Frías-De León, María Guadalupe ; Ramírez, José Antonio ; Taylor, María Lucia ; de León-Garduño, Alfredo Ponce ; Sifuentes-Osornio, José</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c624t-d3c053c0936cc324a277a8979a6742f1f79e6230982544d68d590f2cc07f1ae03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Accuracy</topic><topic>Acquired immune deficiency syndrome</topic><topic>AIDS</topic><topic>Analysis</topic><topic>Antigens</topic><topic>Aspartate aminotransferase</topic><topic>Biology and Life Sciences</topic><topic>Biopsy</topic><topic>Bleeding</topic><topic>Body weight</topic><topic>Body weight loss</topic><topic>Bone marrow</topic><topic>Care and treatment</topic><topic>Cholecystitis</topic><topic>Consortia</topic><topic>Deoxyribonucleic acid</topic><topic>Diagnosis</topic><topic>Diagnostic systems</topic><topic>Diarrhea</topic><topic>Disease</topic><topic>DNA</topic><topic>Enzyme immunoassay</topic><topic>Enzymes</topic><topic>Epididymitis</topic><topic>Ethical standards</topic><topic>Ethics</topic><topic>Ferritin</topic><topic>Fever</topic><topic>Fungal infections</topic><topic>Fungi</topic><topic>Heart</topic><topic>Hemoglobin</topic><topic>Histopathology</topic><topic>Histoplasmosis</topic><topic>HIV</topic><topic>HIV patients</topic><topic>Human immunodeficiency virus</topic><topic>Immunoassay</topic><topic>Immunoassays</topic><topic>Laboratories</topic><topic>Lesions</topic><topic>Leukocytes (neutrophilic)</topic><topic>Lymphadenopathy</topic><topic>Markers</topic><topic>Medical testing products</topic><topic>Medicine and Health Sciences</topic><topic>Mycosis</topic><topic>Observers</topic><topic>Pancreatitis</topic><topic>Pancytopenia</topic><topic>Patients</topic><topic>Physiological aspects</topic><topic>Platelets</topic><topic>Polymerase chain reaction</topic><topic>Prostate</topic><topic>Protocols</topic><topic>Research and Analysis Methods</topic><topic>Skin diseases</topic><topic>Spleen</topic><topic>Splenomegaly</topic><topic>Testing</topic><topic>Tropical diseases</topic><topic>Tuberculosis</topic><topic>Ulcers</topic><topic>Urine</topic><topic>Weight loss</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martínez-Gamboa, Areli</creatorcontrib><creatorcontrib>Niembro-Ortega, María Dolores</creatorcontrib><creatorcontrib>Torres-González, Pedro</creatorcontrib><creatorcontrib>Santiago-Cruz, Janeth</creatorcontrib><creatorcontrib>Velázquez-Zavala, Nancy Guadalupe</creatorcontrib><creatorcontrib>Rangel-Cordero, Andrea</creatorcontrib><creatorcontrib>Crabtree-Ramírez, Brenda</creatorcontrib><creatorcontrib>Gamboa-Domínguez, Armando</creatorcontrib><creatorcontrib>Reyes-Gutiérrez, Edgardo</creatorcontrib><creatorcontrib>Reyes-Terán, Gustavo</creatorcontrib><creatorcontrib>Lozano-Fernandez, Víctor Hugo</creatorcontrib><creatorcontrib>Ahumada-Topete, Víctor Hugo</creatorcontrib><creatorcontrib>Martínez-Ayala, Pedro</creatorcontrib><creatorcontrib>Manríquez-Reyes, Marisol</creatorcontrib><creatorcontrib>Ramírez-Hinojosa, Juan Pablo</creatorcontrib><creatorcontrib>Rodríguez-Zulueta, Patricia</creatorcontrib><creatorcontrib>Hernández-León, Christian</creatorcontrib><creatorcontrib>Ruíz-Quiñones, Jesús</creatorcontrib><creatorcontrib>Rivera-Martínez, Norma Eréndira</creatorcontrib><creatorcontrib>Chaparro-Sánchez, Alberto</creatorcontrib><creatorcontrib>Andrade-Villanueva, Jaime</creatorcontrib><creatorcontrib>González-Hernández, Luz Alicia</creatorcontrib><creatorcontrib>Cruz-Martínez, Sofia</creatorcontrib><creatorcontrib>Flores-Barrientos, Oscar</creatorcontrib><creatorcontrib>Gaytán-Martínez, Jesús Enrique</creatorcontrib><creatorcontrib>Magaña-Aquino, Martín</creatorcontrib><creatorcontrib>Cervantes-Sánchez, Axel</creatorcontrib><creatorcontrib>Olivas-Martínez, Antonio</creatorcontrib><creatorcontrib>Araujo-Meléndez, Javier</creatorcontrib><creatorcontrib>Del Rocío Reyes-Montes, María</creatorcontrib><creatorcontrib>Duarte-Escalante, Esperanza</creatorcontrib><creatorcontrib>Frías-De León, María Guadalupe</creatorcontrib><creatorcontrib>Ramírez, José Antonio</creatorcontrib><creatorcontrib>Taylor, María Lucia</creatorcontrib><creatorcontrib>de León-Garduño, Alfredo Ponce</creatorcontrib><creatorcontrib>Sifuentes-Osornio, José</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PLoS neglected tropical diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Martínez-Gamboa, Areli</au><au>Niembro-Ortega, María Dolores</au><au>Torres-González, Pedro</au><au>Santiago-Cruz, Janeth</au><au>Velázquez-Zavala, Nancy Guadalupe</au><au>Rangel-Cordero, Andrea</au><au>Crabtree-Ramírez, Brenda</au><au>Gamboa-Domínguez, Armando</au><au>Reyes-Gutiérrez, Edgardo</au><au>Reyes-Terán, Gustavo</au><au>Lozano-Fernandez, Víctor Hugo</au><au>Ahumada-Topete, Víctor Hugo</au><au>Martínez-Ayala, Pedro</au><au>Manríquez-Reyes, Marisol</au><au>Ramírez-Hinojosa, Juan Pablo</au><au>Rodríguez-Zulueta, Patricia</au><au>Hernández-León, Christian</au><au>Ruíz-Quiñones, Jesús</au><au>Rivera-Martínez, Norma Eréndira</au><au>Chaparro-Sánchez, Alberto</au><au>Andrade-Villanueva, Jaime</au><au>González-Hernández, Luz Alicia</au><au>Cruz-Martínez, Sofia</au><au>Flores-Barrientos, Oscar</au><au>Gaytán-Martínez, Jesús Enrique</au><au>Magaña-Aquino, Martín</au><au>Cervantes-Sánchez, Axel</au><au>Olivas-Martínez, Antonio</au><au>Araujo-Meléndez, Javier</au><au>Del Rocío Reyes-Montes, María</au><au>Duarte-Escalante, Esperanza</au><au>Frías-De León, María Guadalupe</au><au>Ramírez, José Antonio</au><au>Taylor, María Lucia</au><au>de León-Garduño, Alfredo Ponce</au><au>Sifuentes-Osornio, José</au><au>Gonzalez, Angel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diagnostic accuracy of antigen detection in urine and molecular assays testing in different clinical samples for the diagnosis of progressive disseminated histoplasmosis in patients living with HIV/AIDS: A prospective multicenter study in Mexico</atitle><jtitle>PLoS neglected tropical diseases</jtitle><addtitle>PLoS Negl Trop Dis</addtitle><date>2021-03-01</date><risdate>2021</risdate><volume>15</volume><issue>3</issue><spage>e0009215</spage><epage>e0009215</epage><pages>e0009215-e0009215</pages><issn>1935-2735</issn><issn>1935-2727</issn><eissn>1935-2735</eissn><abstract>The progressive disseminated histoplasmosis (PDH) has been associated with severe disease and high risk of death among people living with HIV (PLWHIV). Therefore, the purpose of this multicenter, prospective, double-blinded study done in ten Mexican hospitals was to determine the diagnostic accuracy of detecting Histoplasma capsulatum antigen in urine using the IMMY ALPHA Histoplasma EIA kit (IAHE), clarus Histoplasma GM Enzyme Immunoassay (cHGEI IMMY) and MiraVista Histoplasma Urine Antigen LFA (MVHUALFA); as well as the Hcp100 and 1281-1283220SCAR nested PCRs in blood, bone-marrow, tissue biopsies and urine.
We included 415 PLWHIV older than 18 years of age with suspicion of PDH. Using as diagnostic standard recovery of H. capsulatum in blood, bone marrow or tissue cultures, or histopathological exam compatible, detected 108 patients (26%, [95%CI, 21.78-30.22]) with proven-PDH. We analyzed 391 urine samples by the IAHE, cHGEI IMMY and MVHUALFA; the sensitivity/specificity values obtained were 67.3% (95% CI, 57.4-76.2) / 96.2% (95% CI, 93.2-98.0) for IAHE, 91.3% (95% CI, 84.2-96.0) / 90.9% (95% CI, 87.0-94.0) for cHGEI IMMY and 90.4% (95% CI, 83.0-95.3) / 92.3% (95% CI, 88.6-95.1) for MVHUALFA. The Hcp100 nested PCR was performed on 393, 343, 75 and 297, blood, bone marrow, tissue and urine samples respectively; the sensitivity/specificity values obtained were 62.9% (95%CI, 53.3-72.5)/ 89.5% (95%CI, 86.0-93.0), 65.9% (95%CI, 56.0-75.8)/ 89.0% (95%CI, 85.2-92.9), 62.1% (95%CI, 44.4-79.7)/ 82.6% (95%CI, 71.7-93.6) and 34.9% (95%CI, 24.8-46.2)/ 67.3% (95%CI, 60.6-73.5) respectively; and 1281-1283220SCAR nested PCR was performed on 392, 344, 75 and 291, respectively; the sensitivity/specificity values obtained were 65.3% (95% CI, 55.9-74.7)/ 58.8% (95%CI, 53.2-64.5), 70.8% (95%CI, 61.3-80.2)/ 52.9% (95%CI, 46.8-59.1), 71.4% (95%CI, 54.7-88.2)/ 40.4% (95%CI, 26.4-54.5) and 18.1% (95%CI, 10.5-28.1)/ 90.4% (95%CI, 85.5-94.0), respectively.
The cHGEI IMMY and MVHUALFA tests showed excellent performance for the diagnosis of PDH in PLWHIV. The integration of these tests in clinical laboratories will certainly impact on early diagnosis and treatment.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>33684128</pmid><doi>10.1371/journal.pntd.0009215</doi><orcidid>https://orcid.org/0000-0002-0905-0835</orcidid><orcidid>https://orcid.org/0000-0002-0505-6340</orcidid><orcidid>https://orcid.org/0000-0002-2279-1558</orcidid><orcidid>https://orcid.org/0000-0003-2640-2916</orcidid><orcidid>https://orcid.org/0000-0002-3160-8698</orcidid><orcidid>https://orcid.org/0000-0001-5395-6418</orcidid><orcidid>https://orcid.org/0000-0003-2613-4878</orcidid><orcidid>https://orcid.org/0000-0003-1948-655X</orcidid><orcidid>https://orcid.org/0000-0001-5094-7890</orcidid><orcidid>https://orcid.org/0000-0002-4609-4415</orcidid><orcidid>https://orcid.org/0000-0002-1983-0384</orcidid><orcidid>https://orcid.org/0000-0001-9822-3496</orcidid><orcidid>https://orcid.org/0000-0002-7163-0566</orcidid><orcidid>https://orcid.org/0000-0002-6315-7860</orcidid><orcidid>https://orcid.org/0000-0003-2779-0068</orcidid><orcidid>https://orcid.org/0000-0001-9584-1360</orcidid><orcidid>https://orcid.org/0000-0002-6716-1234</orcidid><orcidid>https://orcid.org/0000-0002-3866-9995</orcidid><orcidid>https://orcid.org/0000-0001-5011-5939</orcidid><orcidid>https://orcid.org/0000-0002-2587-1123</orcidid><orcidid>https://orcid.org/0000-0002-3773-613X</orcidid><orcidid>https://orcid.org/0000-0002-9545-2522</orcidid><orcidid>https://orcid.org/0000-0002-3723-333X</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1935-2735 |
| ispartof | PLoS neglected tropical diseases, 2021-03, Vol.15 (3), p.e0009215-e0009215 |
| issn | 1935-2735 1935-2727 1935-2735 |
| language | eng |
| recordid | cdi_plos_journals_2513691730 |
| source | DOAJ Directory of Open Access Journals; PubMed Central Open Access; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Accuracy Acquired immune deficiency syndrome AIDS Analysis Antigens Aspartate aminotransferase Biology and Life Sciences Biopsy Bleeding Body weight Body weight loss Bone marrow Care and treatment Cholecystitis Consortia Deoxyribonucleic acid Diagnosis Diagnostic systems Diarrhea Disease DNA Enzyme immunoassay Enzymes Epididymitis Ethical standards Ethics Ferritin Fever Fungal infections Fungi Heart Hemoglobin Histopathology Histoplasmosis HIV HIV patients Human immunodeficiency virus Immunoassay Immunoassays Laboratories Lesions Leukocytes (neutrophilic) Lymphadenopathy Markers Medical testing products Medicine and Health Sciences Mycosis Observers Pancreatitis Pancytopenia Patients Physiological aspects Platelets Polymerase chain reaction Prostate Protocols Research and Analysis Methods Skin diseases Spleen Splenomegaly Testing Tropical diseases Tuberculosis Ulcers Urine Weight loss Yeasts |
| title | Diagnostic accuracy of antigen detection in urine and molecular assays testing in different clinical samples for the diagnosis of progressive disseminated histoplasmosis in patients living with HIV/AIDS: A prospective multicenter study in Mexico |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T08%3A16%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Diagnostic%20accuracy%20of%20antigen%20detection%20in%20urine%20and%20molecular%20assays%20testing%20in%20different%20clinical%20samples%20for%20the%20diagnosis%20of%20progressive%20disseminated%20histoplasmosis%20in%20patients%20living%20with%20HIV/AIDS:%20A%20prospective%20multicenter%20study%20in%20Mexico&rft.jtitle=PLoS%20neglected%20tropical%20diseases&rft.au=Mart%C3%ADnez-Gamboa,%20Areli&rft.date=2021-03-01&rft.volume=15&rft.issue=3&rft.spage=e0009215&rft.epage=e0009215&rft.pages=e0009215-e0009215&rft.issn=1935-2735&rft.eissn=1935-2735&rft_id=info:doi/10.1371/journal.pntd.0009215&rft_dat=%3Cgale_plos_%3EA658713898%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2513691730&rft_id=info:pmid/33684128&rft_galeid=A658713898&rft_doaj_id=oai_doaj_org_article_b93579871f1a49d1a1f7166df3e2a1d2&rfr_iscdi=true |