Development and validation of an assay for detection of Japanese encephalitis virus specific antibody responses

Although immune responses to the Japanese Encephalitis virus (JEV), and the dengue viruses (DENV) have a potential to modulate the immune responses to each other, this has been poorly investigated. Therefore, we developed an ELISA to identify JEV specific, DENV non cross-reactive antibody responses...

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Veröffentlicht in:PloS one 2020-10, Vol.15 (10), p.e0238609-e0238609
Hauptverfasser: Pushpakumara, Pradeep Darshana, Jeewandara, Chandima, Gomes, Laksiri, Perera, Yashodha, Wijewickrama, Ananda, Malavige, Gathsaurie Neelika, Goonesekara, Charitha, Ansari, Aftab A, .
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Sprache:eng
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Zusammenfassung:Although immune responses to the Japanese Encephalitis virus (JEV), and the dengue viruses (DENV) have a potential to modulate the immune responses to each other, this has been poorly investigated. Therefore, we developed an ELISA to identify JEV specific, DENV non cross-reactive antibody responses by identifying JEV specific, highly conserved regions of the virus and proceeded to investigate if the presence of JEV specific antibodies associate with dengue disease severity. 22 JEV specific peptides were identified from highly conserved regions of the virus and the immunogenicity and specificity of these peptides were assessed in individuals who were non-immune to JEV and DENV (JEV.sup.- DENV.sup.-, N = 30), those who were only immune to the JEV and not DENV (JEV.sup.+ DENV.sup.-, N = 30), those who were only immune to DENV(JEV.sup.- DENV.sup.+, N = 30) and in those who were immune to both viruses (JEV.sup.+ DENV.sup.+, N = 30). 7/22 peptides were found to be highly immunogenic and specific and these 7 peptides were used as a pool to further evaluate JEV-specific responses. All 30/30 JEV.sup.+ DENV.sup.- and 30/30 JEV.sup.+ DENV.sup.+ individuals, and only 3/30 (10%) JEV.sup.- DENV.sup.+ individuals responded to this pool. We further evaluated this pool of 7 peptides in patients following primary and secondary dengue infection during the convalescent period and found that the JEV-specific peptides, were unlikely to cross react with DENV IgG antibodies. We further compared this in-house ELISA developed with the peptide pool with an existing commercial JEV IgG assay to identify JEV-specific IgG following vaccination, and our in-house ELISA was found to be more sensitive. We then proceeded to investigate if the presence of JEV-specific antibodies were associated with dengue disease severity, and we found that those who had past severe dengue (n = 175) were significantly more likely (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0238609