Utilizing methylglyoxal and D-lactate in urine to evaluate saikosaponin C treatment in mice with accelerated nephrotoxic serum nephritis
The relationship between methylglyoxal (MGO) and D-lactate during saikosaponin C (SSC) treatment of mice with accelerated nephrotoxic serum (NTS) nephritis was investigated. NTS nephritis was induced by administration of anti-basement membrane antibodies to C57BL/6 mice and three dosages of SSC were...
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| creator | Lin, Chia-Yu Lee, Jen-Ai Lin, Po-Yeh Hua, Shih-Chun Tsai, Pei-Yun Chen, Bi-Li Lin, Chia-En Lee, Tzong-Huei Chen, Shih-Ming |
| description | The relationship between methylglyoxal (MGO) and D-lactate during saikosaponin C (SSC) treatment of mice with accelerated nephrotoxic serum (NTS) nephritis was investigated. NTS nephritis was induced by administration of anti-basement membrane antibodies to C57BL/6 mice and three dosages of SSC were administered for 14 days. Proteinuria, blood urea nitrogen, serum creatinine, renal histology, urinary MGO and d-lactate changes were examined. Compared to the NTS control group, the middle dosage (10 mg/kg/day) of SSC significantly alleviated the development of nephritis based on urine protein measurements (34.40 ± 6.85 vs. 17.33 ± 4.79 mg/day, p |
| doi_str_mv | 10.1371/journal.pone.0241053 |
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NTS nephritis was induced by administration of anti-basement membrane antibodies to C57BL/6 mice and three dosages of SSC were administered for 14 days. Proteinuria, blood urea nitrogen, serum creatinine, renal histology, urinary MGO and d-lactate changes were examined. Compared to the NTS control group, the middle dosage (10 mg/kg/day) of SSC significantly alleviated the development of nephritis based on urine protein measurements (34.40 ± 6.85 vs. 17.33 ± 4.79 mg/day, p<0.05). Pathological observation of the glomerular basement membrane (GBM) revealed monocyte infiltration, hypertrophy, and crescents were alleviated, and injury scoring also showed improved efficacy for the middle dose of SSC during nephritis (7.92 ± 1.37 vs. 3.50 ± 1.14, p<0.05). Moreover, the significant decreases in urinary levels of MGO (24.71 ± 3.46 vs. 16.72 ± 2.36 μg/mg, p<0.05) and D-lactate (0.31 ± 0.04 vs. 0.23 ± 0.02 μmol/mg, p<0.05) were consistent with the biochemical and pathological examinations. This study demonstrates that MGO and D-lactate may reflect the extent of damage and the efficacy of SSC in NTS nephritis; further studies are required to enable clinical application.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0241053</identifier><identifier>PMID: 33104740</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Antibodies ; Antigens ; Biology and Life Sciences ; Care and treatment ; Chromatography ; Creatinine ; Dehydrogenases ; Dosage ; Glomerulonephritis - drug therapy ; Health aspects ; Histology ; Hypertrophy ; Immunoglobulins ; Ketones ; Kidneys ; Laboratory animals ; Lactates ; Lactic acid ; Lactic Acid - urine ; Medicine and Health Sciences ; Membranes ; Metabolism ; Metabolites ; Mice ; Mice, Inbred C57BL ; Monocytes ; Nephritis ; Oleanolic Acid - administration & dosage ; Oleanolic Acid - analogs & derivatives ; Oleanolic Acid - therapeutic use ; Pharmacy ; Physiological aspects ; Proteins ; Proteinuria ; Pyruvaldehyde ; Pyruvaldehyde - urine ; Rabbits ; Saponins ; Saponins - administration & dosage ; Saponins - therapeutic use ; Supervision ; Urea ; Urine</subject><ispartof>PloS one, 2020-10, Vol.15 (10), p.e0241053</ispartof><rights>COPYRIGHT 2020 Public Library of Science</rights><rights>2020 Lin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 Lin et al 2020 Lin et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-318bfbea8ed19fcc75a060dcef708c08216299792a1e0d0b492769b63266c9d23</citedby><cites>FETCH-LOGICAL-c692t-318bfbea8ed19fcc75a060dcef708c08216299792a1e0d0b492769b63266c9d23</cites><orcidid>0000-0002-0273-4847</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7588094/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7588094/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2926,23865,27923,27924,53790,53792,79371,79372</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33104740$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Chadjichristos, Christos E.</contributor><creatorcontrib>Lin, Chia-Yu</creatorcontrib><creatorcontrib>Lee, Jen-Ai</creatorcontrib><creatorcontrib>Lin, Po-Yeh</creatorcontrib><creatorcontrib>Hua, Shih-Chun</creatorcontrib><creatorcontrib>Tsai, Pei-Yun</creatorcontrib><creatorcontrib>Chen, Bi-Li</creatorcontrib><creatorcontrib>Lin, Chia-En</creatorcontrib><creatorcontrib>Lee, Tzong-Huei</creatorcontrib><creatorcontrib>Chen, Shih-Ming</creatorcontrib><title>Utilizing methylglyoxal and D-lactate in urine to evaluate saikosaponin C treatment in mice with accelerated nephrotoxic serum nephritis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The relationship between methylglyoxal (MGO) and D-lactate during saikosaponin C (SSC) treatment of mice with accelerated nephrotoxic serum (NTS) nephritis was investigated. NTS nephritis was induced by administration of anti-basement membrane antibodies to C57BL/6 mice and three dosages of SSC were administered for 14 days. Proteinuria, blood urea nitrogen, serum creatinine, renal histology, urinary MGO and d-lactate changes were examined. Compared to the NTS control group, the middle dosage (10 mg/kg/day) of SSC significantly alleviated the development of nephritis based on urine protein measurements (34.40 ± 6.85 vs. 17.33 ± 4.79 mg/day, p<0.05). Pathological observation of the glomerular basement membrane (GBM) revealed monocyte infiltration, hypertrophy, and crescents were alleviated, and injury scoring also showed improved efficacy for the middle dose of SSC during nephritis (7.92 ± 1.37 vs. 3.50 ± 1.14, p<0.05). Moreover, the significant decreases in urinary levels of MGO (24.71 ± 3.46 vs. 16.72 ± 2.36 μg/mg, p<0.05) and D-lactate (0.31 ± 0.04 vs. 0.23 ± 0.02 μmol/mg, p<0.05) were consistent with the biochemical and pathological examinations. This study demonstrates that MGO and D-lactate may reflect the extent of damage and the efficacy of SSC in NTS nephritis; further studies are required to enable clinical application.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Antigens</subject><subject>Biology and Life Sciences</subject><subject>Care and treatment</subject><subject>Chromatography</subject><subject>Creatinine</subject><subject>Dehydrogenases</subject><subject>Dosage</subject><subject>Glomerulonephritis - drug therapy</subject><subject>Health aspects</subject><subject>Histology</subject><subject>Hypertrophy</subject><subject>Immunoglobulins</subject><subject>Ketones</subject><subject>Kidneys</subject><subject>Laboratory animals</subject><subject>Lactates</subject><subject>Lactic acid</subject><subject>Lactic Acid - urine</subject><subject>Medicine and Health Sciences</subject><subject>Membranes</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Monocytes</subject><subject>Nephritis</subject><subject>Oleanolic Acid - administration & dosage</subject><subject>Oleanolic Acid - analogs & derivatives</subject><subject>Oleanolic Acid - therapeutic use</subject><subject>Pharmacy</subject><subject>Physiological aspects</subject><subject>Proteins</subject><subject>Proteinuria</subject><subject>Pyruvaldehyde</subject><subject>Pyruvaldehyde - urine</subject><subject>Rabbits</subject><subject>Saponins</subject><subject>Saponins - administration & dosage</subject><subject>Saponins - therapeutic use</subject><subject>Supervision</subject><subject>Urea</subject><subject>Urine</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7rr6D0QLguDFjPlo0-ZGWMavgYUFdb0NaXraZk2bMUnXGX-BP9uM012moCC5SDh53vccTnKS5ClGS0wL_Prajm6QZrmxAywRyTDK6b3kFHNKFowgev_ofJI88v4aRaJk7GFyQilGWZGh0-TXVdBG_9RDm_YQup1pzc5upUnlUKdvF0aqIAOkekhHpwdIg03hRppxH_RSf7NexgLi9SoNDmToYQh7utcK0h86dKlUCgy4KKjTATads8FutUo9uLE_RHTQ_nHyoJHGw5NpP0uu3r_7svq4uLj8sF6dXywU4yQsKC6rpgJZQo15o1SRS8RQraApUKlQSTAjnBecSAyoRlXGScF4xShhTPGa0LPk-cF3Y6wXUxO9IFmeUV7mGEVifSBqK6_Fxuleup2wUos_AetaIV3QyoBgoCjLSlmppsyqkuwT5KrIOIovxBGLXm-mbGPVQyxzCE6amen8ZtCdaO2NKPKyRDyLBi8mA2e_j-DDP0qeqFbGqvTQ2Gimeu2VOGeU5wTHFanlX6i4aojPFb9Ro2N8Jng1E0QmwDa0cvRerD9_-n_28uucfXnEdiBN6Lw1Y9B28HMwO4DKWe8dNHedw0jsp-C2G2I_BWKagih7dtz1O9Htt6e_AQifBCc</recordid><startdate>20201026</startdate><enddate>20201026</enddate><creator>Lin, Chia-Yu</creator><creator>Lee, Jen-Ai</creator><creator>Lin, Po-Yeh</creator><creator>Hua, Shih-Chun</creator><creator>Tsai, Pei-Yun</creator><creator>Chen, Bi-Li</creator><creator>Lin, Chia-En</creator><creator>Lee, Tzong-Huei</creator><creator>Chen, Shih-Ming</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-0273-4847</orcidid></search><sort><creationdate>20201026</creationdate><title>Utilizing methylglyoxal and D-lactate in urine to evaluate saikosaponin C treatment in mice with accelerated nephrotoxic serum nephritis</title><author>Lin, Chia-Yu ; Lee, Jen-Ai ; Lin, Po-Yeh ; Hua, Shih-Chun ; Tsai, Pei-Yun ; Chen, Bi-Li ; Lin, Chia-En ; Lee, Tzong-Huei ; Chen, Shih-Ming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-318bfbea8ed19fcc75a060dcef708c08216299792a1e0d0b492769b63266c9d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Antigens</topic><topic>Biology and Life Sciences</topic><topic>Care and treatment</topic><topic>Chromatography</topic><topic>Creatinine</topic><topic>Dehydrogenases</topic><topic>Dosage</topic><topic>Glomerulonephritis - drug therapy</topic><topic>Health aspects</topic><topic>Histology</topic><topic>Hypertrophy</topic><topic>Immunoglobulins</topic><topic>Ketones</topic><topic>Kidneys</topic><topic>Laboratory animals</topic><topic>Lactates</topic><topic>Lactic acid</topic><topic>Lactic Acid - urine</topic><topic>Medicine and Health Sciences</topic><topic>Membranes</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Monocytes</topic><topic>Nephritis</topic><topic>Oleanolic Acid - administration & dosage</topic><topic>Oleanolic Acid - analogs & derivatives</topic><topic>Oleanolic Acid - therapeutic use</topic><topic>Pharmacy</topic><topic>Physiological aspects</topic><topic>Proteins</topic><topic>Proteinuria</topic><topic>Pyruvaldehyde</topic><topic>Pyruvaldehyde - urine</topic><topic>Rabbits</topic><topic>Saponins</topic><topic>Saponins - administration & dosage</topic><topic>Saponins - therapeutic use</topic><topic>Supervision</topic><topic>Urea</topic><topic>Urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, Chia-Yu</creatorcontrib><creatorcontrib>Lee, Jen-Ai</creatorcontrib><creatorcontrib>Lin, Po-Yeh</creatorcontrib><creatorcontrib>Hua, Shih-Chun</creatorcontrib><creatorcontrib>Tsai, Pei-Yun</creatorcontrib><creatorcontrib>Chen, Bi-Li</creatorcontrib><creatorcontrib>Lin, Chia-En</creatorcontrib><creatorcontrib>Lee, Tzong-Huei</creatorcontrib><creatorcontrib>Chen, Shih-Ming</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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NTS nephritis was induced by administration of anti-basement membrane antibodies to C57BL/6 mice and three dosages of SSC were administered for 14 days. Proteinuria, blood urea nitrogen, serum creatinine, renal histology, urinary MGO and d-lactate changes were examined. Compared to the NTS control group, the middle dosage (10 mg/kg/day) of SSC significantly alleviated the development of nephritis based on urine protein measurements (34.40 ± 6.85 vs. 17.33 ± 4.79 mg/day, p<0.05). Pathological observation of the glomerular basement membrane (GBM) revealed monocyte infiltration, hypertrophy, and crescents were alleviated, and injury scoring also showed improved efficacy for the middle dose of SSC during nephritis (7.92 ± 1.37 vs. 3.50 ± 1.14, p<0.05). Moreover, the significant decreases in urinary levels of MGO (24.71 ± 3.46 vs. 16.72 ± 2.36 μg/mg, p<0.05) and D-lactate (0.31 ± 0.04 vs. 0.23 ± 0.02 μmol/mg, p<0.05) were consistent with the biochemical and pathological examinations. This study demonstrates that MGO and D-lactate may reflect the extent of damage and the efficacy of SSC in NTS nephritis; further studies are required to enable clinical application.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>33104740</pmid><doi>10.1371/journal.pone.0241053</doi><tpages>e0241053</tpages><orcidid>https://orcid.org/0000-0002-0273-4847</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Animals Antibodies Antigens Biology and Life Sciences Care and treatment Chromatography Creatinine Dehydrogenases Dosage Glomerulonephritis - drug therapy Health aspects Histology Hypertrophy Immunoglobulins Ketones Kidneys Laboratory animals Lactates Lactic acid Lactic Acid - urine Medicine and Health Sciences Membranes Metabolism Metabolites Mice Mice, Inbred C57BL Monocytes Nephritis Oleanolic Acid - administration & dosage Oleanolic Acid - analogs & derivatives Oleanolic Acid - therapeutic use Pharmacy Physiological aspects Proteins Proteinuria Pyruvaldehyde Pyruvaldehyde - urine Rabbits Saponins Saponins - administration & dosage Saponins - therapeutic use Supervision Urea Urine |
| title | Utilizing methylglyoxal and D-lactate in urine to evaluate saikosaponin C treatment in mice with accelerated nephrotoxic serum nephritis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T04%3A19%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Utilizing%20methylglyoxal%20and%20D-lactate%20in%20urine%20to%20evaluate%20saikosaponin%20C%20treatment%20in%20mice%20with%20accelerated%20nephrotoxic%20serum%20nephritis&rft.jtitle=PloS%20one&rft.au=Lin,%20Chia-Yu&rft.date=2020-10-26&rft.volume=15&rft.issue=10&rft.spage=e0241053&rft.pages=e0241053-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0241053&rft_dat=%3Cgale_plos_%3EA639521212%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2454398510&rft_id=info:pmid/33104740&rft_galeid=A639521212&rft_doaj_id=oai_doaj_org_article_6ec3648abcf84b829d235c7490371906&rfr_iscdi=true |