A LAMP at the end of the tunnel : a rapid, field deployable assay for the kauri dieback pathogen, Phytophthora agathidicida

Notes how the root rot causing oomycete, Phytophthora agathidicida, threatens the long-term survival of the iconic New Zealand kauri tree. Describes a loop-mediated isothermal amplification (LAMP) assay for the detection of P. agathidicida that targets a portion of the mitochondrial apocytochrome b...

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Veröffentlicht in:	PloS one 2020-01, Vol.15 (1), p.e0224007-e0224007
1. Verfasser:	Winkworth, Richard C
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Araucariaceae - genetics Araucariaceae - microbiology Baiting Baits Bioassay Bioassays Biological Assay Biological assays Biology and Life Sciences Conservation Deoxyribonucleic acid Dieback Disease control Diseases and pests DNA DNA, Plant - genetics Ecology and Environmental Sciences Flooded soils Floods Forest declines Forest soils Genetic testing Genomes Identification Information management Infrastructure (Economics) Kauri Laboratories Local communities Mitochondria New Zealand Pathogens Photographic industry Phytophthora Phytophthora - genetics Phytophthora - isolation & purification Phytophthora - pathogenicity Phytophthora diseases Plant Diseases - genetics Plant Diseases - microbiology Plant tissues Research and analysis methods Ribosomal DNA Root rot Sensitivity Signs and symptoms Soil investigations Soil Microbiology Soil testing Soils Trees
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Beschreibung
Zusammenfassung:	Notes how the root rot causing oomycete, Phytophthora agathidicida, threatens the long-term survival of the iconic New Zealand kauri tree. Describes a loop-mediated isothermal amplification (LAMP) assay for the detection of P. agathidicida that targets a portion of the mitochondrial apocytochrome b coding sequence. Discusses the various ways such an assay can be used in the communities affected. Source: National Library of New Zealand Te Puna Matauranga o Aotearoa, licensed by the Department of Internal Affairs for re-use under the Creative Commons Attribution 3.0 New Zealand Licence.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0224007