Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing
Fragmentation of DNA is the very important first step in preparing nucleic acids for next-generation sequencing. Here we report a novel Fragmentation Through Polymerization (FTP) technique, which is a simple, robust, and low-cost enzymatic method of fragmentation. This method generates double-strand...
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| Veröffentlicht in: | PloS one 2019-04, Vol.14 (4), p.e0210374-e0210374 |
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| creator | Ignatov, Konstantin B Blagodatskikh, Konstantin A Shcherbo, Dmitry S Kramarova, Tatiana V Monakhova, Yulia A Kramarov, Vladimir M |
| description | Fragmentation of DNA is the very important first step in preparing nucleic acids for next-generation sequencing. Here we report a novel Fragmentation Through Polymerization (FTP) technique, which is a simple, robust, and low-cost enzymatic method of fragmentation. This method generates double-stranded DNA fragments that are suitable for direct use in NGS library construction and allows the elimination of the additional step of reparation of DNA ends. |
| doi_str_mv | 10.1371/journal.pone.0210374 |
| format | Article |
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Here we report a novel Fragmentation Through Polymerization (FTP) technique, which is a simple, robust, and low-cost enzymatic method of fragmentation. 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Here we report a novel Fragmentation Through Polymerization (FTP) technique, which is a simple, robust, and low-cost enzymatic method of fragmentation. This method generates double-stranded DNA fragments that are suitable for direct use in NGS library construction and allows the elimination of the additional step of reparation of DNA ends.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>30933980</pmid><doi>10.1371/journal.pone.0210374</doi><orcidid>https://orcid.org/0000-0003-3818-6560</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acoustics Agricultural biotechnology Analysis Bias Binding sites Bioinformatics Biology and life sciences Computational Biology Deoxyribonuclease I - chemistry Deoxyribonucleic acid DNA DNA - chemistry DNA - genetics DNA Fragmentation DNA polymerase DNA sequencing EDTA Enzymes Fragmentation Gene Library Gene sequencing Genetic testing Genomes High-Throughput Nucleotide Sequencing - methods Methods Next-generation sequencing Novels Nucleic acids Polymerization Research and analysis methods Resveratrol Sequence Analysis, DNA - methods Technology |
| title | Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing |
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