Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing

Fragmentation of DNA is the very important first step in preparing nucleic acids for next-generation sequencing. Here we report a novel Fragmentation Through Polymerization (FTP) technique, which is a simple, robust, and low-cost enzymatic method of fragmentation. This method generates double-strand...

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Veröffentlicht in:	PloS one 2019-04, Vol.14 (4), p.e0210374-e0210374
Hauptverfasser:	Ignatov, Konstantin B, Blagodatskikh, Konstantin A, Shcherbo, Dmitry S, Kramarova, Tatiana V, Monakhova, Yulia A, Kramarov, Vladimir M
Format:	Artikel
Sprache:	eng
Schlagworte:	Acoustics Agricultural biotechnology Analysis Bias Binding sites Bioinformatics Biology and life sciences Computational Biology Deoxyribonuclease I - chemistry Deoxyribonucleic acid DNA DNA - chemistry DNA - genetics DNA Fragmentation DNA polymerase DNA sequencing EDTA Enzymes Fragmentation Gene Library Gene sequencing Genetic testing Genomes High-Throughput Nucleotide Sequencing - methods Methods Next-generation sequencing Novels Nucleic acids Polymerization Research and analysis methods Resveratrol Sequence Analysis, DNA - methods Technology
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Beschreibung
Zusammenfassung:	Fragmentation of DNA is the very important first step in preparing nucleic acids for next-generation sequencing. Here we report a novel Fragmentation Through Polymerization (FTP) technique, which is a simple, robust, and low-cost enzymatic method of fragmentation. This method generates double-stranded DNA fragments that are suitable for direct use in NGS library construction and allows the elimination of the additional step of reparation of DNA ends.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0210374