Epstein-Barr virus infection and variants of Epstein-Barr nuclear antigen-1 in synovial　tissues of rheumatoid arthritis

Epstein-Barr virus infection and variants of Epstein-Barr nuclear antigen-1 in synovial　tissues of rheumatoid arthritis

The objective of the present study was to investigate Epstein-Barr virus (EBV) infection as an environmental factor for the development of rheumatoid arthritis (RA). Synovial tissues were collected during surgery from 128 RA and 98 osteoarthritis (OA) patients. DNA was extracted from synovial tissue...

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Veröffentlicht in:PloS one 2018-12, Vol.13 (12), p.e0208957-e0208957
Hauptverfasser: Masuoka, Shotaro, Kusunoki, Natsuko, Takamatsu, Ryo, Takahashi, Hiroshi, Tsuchiya, Kazuaki, Kawai, Shinichi, Nanki, Toshihiro
Format: Artikel
Sprache:eng
Schlagworte:
Antigens
Arthritis
Arthritis, Rheumatoid - genetics
Arthritis, Rheumatoid - pathology
Arthritis, Rheumatoid - virology
Biocompatibility
Biology and Life Sciences
Biomedical materials
Bone surgery
Chronic infection
Cytomegalovirus
Deoxyribonucleic acid
DNA
DNA polymerase
Drb1 protein
EBNA-1 protein
Ecological risk assessment
Environmental factors
Environmental risk
Epitopes
Epitopes - genetics
Epitopes - immunology
Epstein-Barr virus
Epstein-Barr Virus Infections - genetics
Epstein-Barr Virus Infections - physiopathology
Epstein-Barr Virus Infections - virology
Epstein-Barr Virus Nuclear Antigens - genetics
Epstein-Barr Virus Nuclear Antigens - immunology
Epstein-Barr Virus Nuclear Antigens - isolation & purification
Female
Genotyping
Herpesvirus 4, Human - genetics
Herpesvirus 4, Human - isolation & purification
Herpesvirus 4, Human - pathogenicity
Histocompatibility antigen HLA
HLA-DRB1 Chains - genetics
HLA-DRB1 Chains - immunology
Humans
Immune system
Infections
Inflammation
Internal medicine
Lymphoma
Male
Medicine
Medicine and Health Sciences
Nucleotide sequence
Orthopedics
Osteoarthritis
Osteoarthritis - genetics
Osteoarthritis - physiopathology
Osteoarthritis - virology
Patients
Polymerase chain reaction
Proteins
Research and Analysis Methods
Rheumatoid arthritis
Rheumatology
Risk analysis
Risk Factors
Surgery
Synovial Fluid - virology
Tissues
Tumor necrosis factor-TNF
Viruses
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Zusammenfassung:The objective of the present study was to investigate Epstein-Barr virus (EBV) infection as an environmental factor for the development of rheumatoid arthritis (RA). Synovial tissues were collected during surgery from 128 RA and 98 osteoarthritis (OA) patients. DNA was extracted from synovial tissues. The EBV gene was assessed by nested PCR for the amplification of EBV nuclear antigen-1 (EBNA-1). The nucleotide sequence of the PCR product was elucidated. HLA-DRB1 genotyping was also performed. EBV DNA was more frequently detected in the synovial tissues of RA patients (32.8%) than OA patients (15.3%) (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0208957