Validation of the blood gas analyzer for pH measurements in IVF culture medium: Prevent suboptimal culture conditions

Measurement of pH in IVF-media using the blood gas analyzer (BGA) requires validation, because IVF-media is outside the intended scope of the BGA. To determine whether the Siemens Rapidpoint 500 BGA is suitable for pH measurements in IVF-media this study will validate the BGA and assess its accuracy...

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Veröffentlicht in:	PloS one 2018-11, Vol.13 (11), p.e0206707-e0206707
Hauptverfasser:	Diaz de Pool, Juan D N, Van Den Berg, Sjoerd A A, Pilgram, Gonneke S K, Ballieux, Bartholomeus E P B, Van Der Westerlaken, Lucia A J
Format:	Artikel
Sprache:	eng
Schlagworte:	Accuracy Biology and Life Sciences Blood Calibration Carbon dioxide Coefficient of variation Culture Culture media Electrodes Embryos Engineering and Technology Gas analyzers Gynecology In vitro fertilization Laboratories Medical ethics Medicine and Health Sciences Methods pH effects Reproducibility Research and Analysis Methods
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Beschreibung
Zusammenfassung:	Measurement of pH in IVF-media using the blood gas analyzer (BGA) requires validation, because IVF-media is outside the intended scope of the BGA. To determine whether the Siemens Rapidpoint 500 BGA is suitable for pH measurements in IVF-media this study will validate the BGA and assess its accuracy. In this method comparison study, the pH of over three hundred IVF-media samples was measured with the BGA and a pH electrode (Hanna pH checker). The precision of both the BGA and the pH electrode were excellent (coefficient variation
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0206707