Performance of cryptococcal antigen lateral flow assay using saliva in Ugandans with CD4 <100

Cryptococcal meningitis can best be diagnosed by cerebrospinal fluid India ink microscopy, cryptococcal antigen detection, or culture. These require invasive lumbar punctures. The utility of cryptococcal antigen detection in saliva is unknown. We evaluated the diagnostic performance of the point-of-...

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Veröffentlicht in:	PloS one 2014-07, Vol.9 (7), p.e103156-e103156
Hauptverfasser:	Kwizera, Richard, Nguna, Joyce, Kiragga, Agnes, Nakavuma, Jesca, Rajasingham, Radha, Boulware, David R, Meya, David B
Format:	Artikel
Sprache:	eng
Schlagworte:	Acquired immune deficiency syndrome Adult Adults AIDS Antigens Antigens, Fungal - analysis Antiretroviral agents Antiretroviral therapy Assaying Biology and Life Sciences CD4 antigen CD4 Lymphocyte Count Cell culture Cerebrospinal fluid Comparative analysis Consent Cost analysis Cryptococcosis Cryptococcus - immunology Diagnostic systems Drug therapy Female Fungal infections Highly active antiretroviral therapy HIV HIV Infections - complications HIV Infections - immunology HIV tests Hospitals Human immunodeficiency virus Humans Infectious diseases Laboratories Male Medicine Medicine and Health Sciences Meningitis Meningitis, Cryptococcal - diagnosis Meningitis, Cryptococcal - immunology Microscopy Middle Aged Mycoses Patients Peripheral blood Plasma Preempting Printing inks Research and Analysis Methods Saliva Saliva - immunology Sensitivity and Specificity Uganda Urine
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Zusammenfassung:	Cryptococcal meningitis can best be diagnosed by cerebrospinal fluid India ink microscopy, cryptococcal antigen detection, or culture. These require invasive lumbar punctures. The utility of cryptococcal antigen detection in saliva is unknown. We evaluated the diagnostic performance of the point-of-care cryptococcal antigen lateral flow assay (CrAg LFA) in saliva. We screened HIV-infected, antiretroviral therapy naïve persons with symptomatic meningitis (n = 130) and asymptomatic persons with CD4+
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0103156