Mechanisms of Intestinal Serotonin Transporter (SERT) Upregulation by TGF-β1 Induced Non-Smad Pathways

TGF-β1 is an important multifunctional cytokine with numerous protective effects on intestinal mucosa. The influence of TGF-β1 on serotonin transporter (SERT) activity, the critical mechanism regulating the extracellular availability of serotonin (5-HT), is not known. Current studies were designed t...

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Veröffentlicht in:	PloS one 2015-05, Vol.10 (5), p.e0120447-e0120447
Hauptverfasser:	Nazir, Saad, Kumar, Anoop, Chatterjee, Ishita, Anbazhagan, Arivarasu N, Gujral, Tarunmeet, Priyamvada, Shubha, Saksena, Seema, Alrefai, Waddah A, Dudeja, Pradeep K, Gill, Ravinder K
Format:	Artikel
Sprache:	eng
Schlagworte:	1-Phosphatidylinositol 3-kinase Activation Acute effects Animals Brefeldin A Caco-2 Cells Cell culture Cysts Diarrhea Disorders Epidermal growth factor Exocytosis Gastroenterology Hepatology Humans Ileum Inflammation Inflammatory bowel disease Inhibitors Intestinal Mucosa - metabolism Intestine Kinases Medicine Mice, Inbred C57BL Mucosa N-Ethylmaleimide-sensitive protein Phosphatidylinositol 3-Kinases - metabolism Physiology Protein Interaction Maps Protein Transport Proteins Qa-SNARE Proteins - metabolism Receptors, Transforming Growth Factor beta - metabolism Rodents Serotonin Serotonin - metabolism Serotonin Plasma Membrane Transport Proteins - genetics Serotonin Plasma Membrane Transport Proteins - metabolism Serotonin transporter Signal Transduction Smad protein SNAP receptors Stimulation Studies Syntaxin Three dimensional models Transfection Transforming Growth Factor beta1 - metabolism Transforming growth factor-b1 Up-Regulation
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Zusammenfassung:	TGF-β1 is an important multifunctional cytokine with numerous protective effects on intestinal mucosa. The influence of TGF-β1 on serotonin transporter (SERT) activity, the critical mechanism regulating the extracellular availability of serotonin (5-HT), is not known. Current studies were designed to examine acute effects of TGF-β1 on SERT. Model human intestinal Caco-2 cells grown as monolayer's or as cysts in 3D culture and ex vivo mouse model were utilized. Treatment of Caco-2 cells with TGF-β1 (10 ng/ml, 60 min) stimulated SERT activity (~2 fold, P
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0120447