Low expression of miR-196b enhances the expression of BCR-ABL1 and HOXA9 oncogenes in chronic myeloid leukemogenesis

MicroRNAs (miRNAs) can function as tumor suppressors or oncogene promoters during tumor development. In this study, low levels of expression of miR-196b were detected in patients with chronic myeloid leukemia. Bisulfite genomic sequencing PCR and methylation-specific PCR were used to examine the met...

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Veröffentlicht in:	PloS one 2013-07, Vol.8 (7), p.e68442
Hauptverfasser:	Liu, Yue, Zheng, Wenling, Song, Yanbin, Ma, Wenli, Yin, Hong
Format:	Artikel
Sprache:	eng
Schlagworte:	Bioinformatics Biology Bisulfite Bone marrow Cancer Cancer genetics Cell cycle Cell Line, Tumor Cell proliferation Cell Transformation, Neoplastic - genetics Chronic myeloid leukemia CpG Islands Deoxyribonucleic acid DNA DNA Methylation Epigenetics Fusion Proteins, bcr-abl - genetics Gene Expression Gene Expression Regulation, Leukemic Genes Genes, Reporter Genetic aspects Genetic engineering Genomes Genomics Homeodomain Proteins - genetics HOXA9 protein Humans Islands K562 Cells Leukemia Leukemia, Myelogenous, Chronic, BCR-ABL Positive - genetics Leukemogenesis Low level Luciferase Medical research Medicine Methylation MicroRNA MicroRNAs MicroRNAs - genetics miRNA Mutation Myeloid leukemia Oncogenes Patients Polymerase chain reaction Promoter Regions, Genetic Reproducibility of Results RNA Interference Stem cells Sulfites Suppressors
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Zusammenfassung:	MicroRNAs (miRNAs) can function as tumor suppressors or oncogene promoters during tumor development. In this study, low levels of expression of miR-196b were detected in patients with chronic myeloid leukemia. Bisulfite genomic sequencing PCR and methylation-specific PCR were used to examine the methylation status of the CpG islands in the miR-196b promoter in K562 cells, patients with leukemia and healthy individuals. The CpG islands showed more methylation in patients with chronic myeloid leukemia compared with healthy individuals (P
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0068442