RNA-Seq Reveals the Angiogenesis Diversity between the Fetal and Adults Bone Mesenchyme Stem Cell

In this research, we used RNA sequencing (RNA-seq) to analyze 23 single cell samples and 2 bulk cells sample from human adult bone mesenchyme stem cell line and human fetal bone mesenchyme stem cell line. The results from the research demonstrated that there were big differences between two cell lin...

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Veröffentlicht in:	PloS one 2016-02, Vol.11 (2), p.e0149171-e0149171
Hauptverfasser:	Zhao, Xin, Han, Yingmin, Liang, Yu, Nie, Chao, Wang, Jian
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Adult Adult Stem Cells - cytology Adult Stem Cells - metabolism Adults Angiogenesis Bioinformatics Biology and Life Sciences Biotechnology Cell lines Cells, Cultured Deoxyribonucleic acid DNA Female Fetus - cytology Fetus - metabolism Fetuses Fibroblast growth factor 2 Gene expression Gene Expression Regulation Gene sequencing Genes Genomes Genomics Humans Hypoxia Liver cancer Male Medicine and Health Sciences Mesenchymal stem cells Mesenchymal Stem Cells - cytology Mesenchymal Stem Cells - metabolism Mesenchyme Middle Aged Neovascularization, Physiologic People and Places Research and Analysis Methods Ribonucleic acid RNA Stem cells Vascular endothelial growth factor
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Zusammenfassung:	In this research, we used RNA sequencing (RNA-seq) to analyze 23 single cell samples and 2 bulk cells sample from human adult bone mesenchyme stem cell line and human fetal bone mesenchyme stem cell line. The results from the research demonstrated that there were big differences between two cell lines. Adult bone mesenchyme stem cell lines showed a strong trend on the blood vessel differentiation and cell motion, 48/49 vascular related differential expressed genes showed higher expression in adult bone mesenchyme stem cell lines (Abmsc) than fetal bone mesenchyme stem cell lines (Fbmsc). 96/106 cell motion related genes showed the same tendency. Further analysis showed that genes like ANGPT1, VEGFA, FGF2, PDGFB and PDGFRA showed higher expression in Abmsc. This work showed cell heterogeneity between human adult bone mesenchyme stem cell line and human fetal bone mesenchyme stem cell line. Also the work may give an indication that Abmsc had a better potency than Fbmsc in the future vascular related application.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0149171