Sensitive commercial NASBA assay for the detection of respiratory syncytial virus in clinical specimen

The aim of the study was to evaluate the usability of three diagnostic procedures for the detection of respiratory syncytial virus in clinical samples. Therefore, the FDA cleared CE marked NOW(R) RSV ELISA, the NucliSENS EasyQ RSV A+B NASBA, and a literature based inhouse RT-PCR protocol were compar...

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Veröffentlicht in:	PloS one 2007-12, Vol.2 (12), p.e1357-e1357
Hauptverfasser:	Tillmann, Ramona Liza, Simon, Arne, Müller, Andreas, Schildgen, Oliver
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids Base Sequence Child Children & youth Cohort Studies Diagnostic systems DNA Primers Enzyme-Linked Immunosorbent Assay Enzymes Humans Immunoassay Infections Infectious Diseases/Respiratory Infections Infectious Diseases/Viral Infections Influenza Intensive care Laboratories Lung diseases Medical diagnosis Pediatrics Polymerase chain reaction Respiratory Medicine/Respiratory Infections Respiratory Medicine/Respiratory Pediatrics Respiratory syncytial virus Respiratory Syncytial Virus Infections - virology Respiratory Syncytial Viruses - isolation & purification Reverse Transcriptase Polymerase Chain Reaction Sensitivity and Specificity Virology Virology/Diagnosis Viruses
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Beschreibung
Zusammenfassung:	The aim of the study was to evaluate the usability of three diagnostic procedures for the detection of respiratory syncytial virus in clinical samples. Therefore, the FDA cleared CE marked NOW(R) RSV ELISA, the NucliSENS EasyQ RSV A+B NASBA, and a literature based inhouse RT-PCR protocol were compared for their relative sensitivities. Thereby, NASBA turned out to be the most sensitive method with a total number of 80 RSV positive samples out of a cohort of 251 nasopharyngeal washings from patients suffering from clinical symptoms, followed by the inhouse RT-PCR (62/251) and ELISA (52/251). Thus, NASBA may serve as a rapid and highly sensitive alternative for RSV diagnostics.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0001357