Expression of ribosomal proteins in normal and cancerous human prostate tissue
Few quantifiable tissue biomarkers for the diagnosis and prognosis of prostate cancer exist. Using an unbiased, quantitative approach, this study evaluates the potential of three proteins of the 40S ribosomal protein complex as putative biomarkers of malignancy in prostate cancer. Prostate tissue ar...
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| description | Few quantifiable tissue biomarkers for the diagnosis and prognosis of prostate cancer exist. Using an unbiased, quantitative approach, this study evaluates the potential of three proteins of the 40S ribosomal protein complex as putative biomarkers of malignancy in prostate cancer. Prostate tissue arrays, constructed from 82 patient samples (245 tissue cores, stage pT3a or pT3b), were stained for antibodies against three ribosomal proteins, RPS19, RPS21 and RPS24. Semi-automated Ox-DAB signal quantification using ImageJ software revealed a significant change in expression of RPS19, RPS21 and RPS24 in malignant vs non-malignant tissue (p |
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Using an unbiased, quantitative approach, this study evaluates the potential of three proteins of the 40S ribosomal protein complex as putative biomarkers of malignancy in prostate cancer. Prostate tissue arrays, constructed from 82 patient samples (245 tissue cores, stage pT3a or pT3b), were stained for antibodies against three ribosomal proteins, RPS19, RPS21 and RPS24. Semi-automated Ox-DAB signal quantification using ImageJ software revealed a significant change in expression of RPS19, RPS21 and RPS24 in malignant vs non-malignant tissue (p<0.0001). Receiver operating characteristics curves were calculated to evaluate the potential of each protein as a biomarker of malignancy in prostate cancer. Positive likelihood ratios for RPS19, RPS21 and RPS24 were calculated as 2.99, 4.21, and 2.56 respectively, indicating that the overexpression of the protein is correlated with the presence of disease. Triple-labelled, quantitative, immunofluorescence (with RPS19, RPS21 and RPS24) showed significant changes (p<0.01) in the global intersection coefficient, a measure of how often two fluorophore signals intersect, for RPS19 and RPS24 only. No change was observed in the co-localization of any other permutations of the three proteins. Our results show that RPS19, RPS21 or RPS24 are upregulated in malignant tissue and may serve as putative biomarkers for prostate cancer.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0186047</identifier><identifier>PMID: 29016636</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aged ; Anemia ; Antibodies ; Biology and Life Sciences ; Biomarkers ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; Biopsy ; Cancer ; Cores ; Development and progression ; Fluorescent Antibody Technique ; Gene Expression Regulation, Neoplastic ; Genes ; Genetic aspects ; Hospitals ; Humans ; Image Interpretation, Computer-Assisted ; Immunofluorescence ; Localization ; Male ; Malignancy ; Mathematical analysis ; Medical diagnosis ; Medical research ; Medicine ; Medicine and Health Sciences ; Middle Aged ; Neoplasm Staging ; Pathology ; Patients ; Permutations ; Physiological aspects ; Prostate - metabolism ; Prostate - pathology ; Prostate cancer ; Prostatic Neoplasms - diagnosis ; Prostatic Neoplasms - genetics ; Prostatic Neoplasms - metabolism ; Prostatic Neoplasms - pathology ; Protein expression ; Proteins ; Research and Analysis Methods ; Retrospective Studies ; Ribosomal DNA ; Ribosomal proteins ; Ribosomal Proteins - genetics ; Ribosomal Proteins - metabolism ; Ribosomes - genetics ; Ribosomes - metabolism ; Ribosomes - pathology ; ROC Curve ; Stem cells ; Surgery ; Tissue Array Analysis ; Tissues ; Urology ; Womens health</subject><ispartof>PloS one, 2017-10, Vol.12 (10), p.e0186047-e0186047</ispartof><rights>COPYRIGHT 2017 Public Library of Science</rights><rights>2017 Arthurs et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 Arthurs et al 2017 Arthurs et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-6f68619040be0c6789554b172a18015ab28a470486395135eff53dcafd33a37e3</citedby><cites>FETCH-LOGICAL-c758t-6f68619040be0c6789554b172a18015ab28a470486395135eff53dcafd33a37e3</cites><orcidid>0000-0001-7405-5336</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5634644/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5634644/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29016636$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Arthurs, Callum</creatorcontrib><creatorcontrib>Murtaza, Bibi Nazia</creatorcontrib><creatorcontrib>Thomson, Calum</creatorcontrib><creatorcontrib>Dickens, Kerry</creatorcontrib><creatorcontrib>Henrique, Rui</creatorcontrib><creatorcontrib>Patel, Hitendra R H</creatorcontrib><creatorcontrib>Beltran, Mariana</creatorcontrib><creatorcontrib>Millar, Michael</creatorcontrib><creatorcontrib>Thrasivoulou, Christopher</creatorcontrib><creatorcontrib>Ahmed, Aamir</creatorcontrib><title>Expression of ribosomal proteins in normal and cancerous human prostate tissue</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Few quantifiable tissue biomarkers for the diagnosis and prognosis of prostate cancer exist. Using an unbiased, quantitative approach, this study evaluates the potential of three proteins of the 40S ribosomal protein complex as putative biomarkers of malignancy in prostate cancer. Prostate tissue arrays, constructed from 82 patient samples (245 tissue cores, stage pT3a or pT3b), were stained for antibodies against three ribosomal proteins, RPS19, RPS21 and RPS24. Semi-automated Ox-DAB signal quantification using ImageJ software revealed a significant change in expression of RPS19, RPS21 and RPS24 in malignant vs non-malignant tissue (p<0.0001). Receiver operating characteristics curves were calculated to evaluate the potential of each protein as a biomarker of malignancy in prostate cancer. Positive likelihood ratios for RPS19, RPS21 and RPS24 were calculated as 2.99, 4.21, and 2.56 respectively, indicating that the overexpression of the protein is correlated with the presence of disease. Triple-labelled, quantitative, immunofluorescence (with RPS19, RPS21 and RPS24) showed significant changes (p<0.01) in the global intersection coefficient, a measure of how often two fluorophore signals intersect, for RPS19 and RPS24 only. No change was observed in the co-localization of any other permutations of the three proteins. Our results show that RPS19, RPS21 or RPS24 are upregulated in malignant tissue and may serve as putative biomarkers for prostate cancer.</description><subject>Aged</subject><subject>Anemia</subject><subject>Antibodies</subject><subject>Biology and Life Sciences</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Biopsy</subject><subject>Cancer</subject><subject>Cores</subject><subject>Development and progression</subject><subject>Fluorescent Antibody Technique</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Image Interpretation, Computer-Assisted</subject><subject>Immunofluorescence</subject><subject>Localization</subject><subject>Male</subject><subject>Malignancy</subject><subject>Mathematical analysis</subject><subject>Medical diagnosis</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Medicine and Health Sciences</subject><subject>Middle Aged</subject><subject>Neoplasm Staging</subject><subject>Pathology</subject><subject>Patients</subject><subject>Permutations</subject><subject>Physiological aspects</subject><subject>Prostate - 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Using an unbiased, quantitative approach, this study evaluates the potential of three proteins of the 40S ribosomal protein complex as putative biomarkers of malignancy in prostate cancer. Prostate tissue arrays, constructed from 82 patient samples (245 tissue cores, stage pT3a or pT3b), were stained for antibodies against three ribosomal proteins, RPS19, RPS21 and RPS24. Semi-automated Ox-DAB signal quantification using ImageJ software revealed a significant change in expression of RPS19, RPS21 and RPS24 in malignant vs non-malignant tissue (p<0.0001). Receiver operating characteristics curves were calculated to evaluate the potential of each protein as a biomarker of malignancy in prostate cancer. Positive likelihood ratios for RPS19, RPS21 and RPS24 were calculated as 2.99, 4.21, and 2.56 respectively, indicating that the overexpression of the protein is correlated with the presence of disease. Triple-labelled, quantitative, immunofluorescence (with RPS19, RPS21 and RPS24) showed significant changes (p<0.01) in the global intersection coefficient, a measure of how often two fluorophore signals intersect, for RPS19 and RPS24 only. No change was observed in the co-localization of any other permutations of the three proteins. Our results show that RPS19, RPS21 or RPS24 are upregulated in malignant tissue and may serve as putative biomarkers for prostate cancer.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>29016636</pmid><doi>10.1371/journal.pone.0186047</doi><tpages>e0186047</tpages><orcidid>https://orcid.org/0000-0001-7405-5336</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | PloS one, 2017-10, Vol.12 (10), p.e0186047-e0186047 |
| issn | 1932-6203 1932-6203 |
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| subjects | Aged Anemia Antibodies Biology and Life Sciences Biomarkers Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Biopsy Cancer Cores Development and progression Fluorescent Antibody Technique Gene Expression Regulation, Neoplastic Genes Genetic aspects Hospitals Humans Image Interpretation, Computer-Assisted Immunofluorescence Localization Male Malignancy Mathematical analysis Medical diagnosis Medical research Medicine Medicine and Health Sciences Middle Aged Neoplasm Staging Pathology Patients Permutations Physiological aspects Prostate - metabolism Prostate - pathology Prostate cancer Prostatic Neoplasms - diagnosis Prostatic Neoplasms - genetics Prostatic Neoplasms - metabolism Prostatic Neoplasms - pathology Protein expression Proteins Research and Analysis Methods Retrospective Studies Ribosomal DNA Ribosomal proteins Ribosomal Proteins - genetics Ribosomal Proteins - metabolism Ribosomes - genetics Ribosomes - metabolism Ribosomes - pathology ROC Curve Stem cells Surgery Tissue Array Analysis Tissues Urology Womens health |
| title | Expression of ribosomal proteins in normal and cancerous human prostate tissue |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T19%3A00%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20of%20ribosomal%20proteins%20in%20normal%20and%20cancerous%20human%20prostate%20tissue&rft.jtitle=PloS%20one&rft.au=Arthurs,%20Callum&rft.date=2017-10-10&rft.volume=12&rft.issue=10&rft.spage=e0186047&rft.epage=e0186047&rft.pages=e0186047-e0186047&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0186047&rft_dat=%3Cgale_plos_%3EA508945258%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1949588568&rft_id=info:pmid/29016636&rft_galeid=A508945258&rft_doaj_id=oai_doaj_org_article_48964786699a42ed90a19ecc0eebad16&rfr_iscdi=true |