Expression of ribosomal proteins in normal and cancerous human prostate tissue

Few quantifiable tissue biomarkers for the diagnosis and prognosis of prostate cancer exist. Using an unbiased, quantitative approach, this study evaluates the potential of three proteins of the 40S ribosomal protein complex as putative biomarkers of malignancy in prostate cancer. Prostate tissue ar...

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Veröffentlicht in:	PloS one 2017-10, Vol.12 (10), p.e0186047-e0186047
Hauptverfasser:	Arthurs, Callum, Murtaza, Bibi Nazia, Thomson, Calum, Dickens, Kerry, Henrique, Rui, Patel, Hitendra R H, Beltran, Mariana, Millar, Michael, Thrasivoulou, Christopher, Ahmed, Aamir
Format:	Artikel
Sprache:	eng
Schlagworte:	Aged Anemia Antibodies Biology and Life Sciences Biomarkers Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Biopsy Cancer Cores Development and progression Fluorescent Antibody Technique Gene Expression Regulation, Neoplastic Genes Genetic aspects Hospitals Humans Image Interpretation, Computer-Assisted Immunofluorescence Localization Male Malignancy Mathematical analysis Medical diagnosis Medical research Medicine Medicine and Health Sciences Middle Aged Neoplasm Staging Pathology Patients Permutations Physiological aspects Prostate - metabolism Prostate - pathology Prostate cancer Prostatic Neoplasms - diagnosis Prostatic Neoplasms - genetics Prostatic Neoplasms - metabolism Prostatic Neoplasms - pathology Protein expression Proteins Research and Analysis Methods Retrospective Studies Ribosomal DNA Ribosomal proteins Ribosomal Proteins - genetics Ribosomal Proteins - metabolism Ribosomes - genetics Ribosomes - metabolism Ribosomes - pathology ROC Curve Stem cells Surgery Tissue Array Analysis Tissues Urology Womens health
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Zusammenfassung:	Few quantifiable tissue biomarkers for the diagnosis and prognosis of prostate cancer exist. Using an unbiased, quantitative approach, this study evaluates the potential of three proteins of the 40S ribosomal protein complex as putative biomarkers of malignancy in prostate cancer. Prostate tissue arrays, constructed from 82 patient samples (245 tissue cores, stage pT3a or pT3b), were stained for antibodies against three ribosomal proteins, RPS19, RPS21 and RPS24. Semi-automated Ox-DAB signal quantification using ImageJ software revealed a significant change in expression of RPS19, RPS21 and RPS24 in malignant vs non-malignant tissue (p
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0186047