The thermodynamics of Pr55Gag-RNA interaction regulate the assembly of HIV

The interactions that occur during HIV Pr55Gag oligomerization and genomic RNA packaging are essential elements that facilitate HIV assembly. However, mechanistic details of these interactions are not clearly defined. Here, we overcome previous limitations in producing large quantities of full-lengt...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	PLoS pathogens 2017-02, Vol.13 (2), p.e1006221-e1006221
Hauptverfasser:	Tanwar, Hanumant S, Khoo, Keith K, Garvey, Megan, Waddington, Lynne, Leis, Andrew, Hijnen, Marcel, Velkov, Tony, Dumsday, Geoff J, McKinstry, William J, Mak, Johnson
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids Amino Acid Sequence Base Sequence Biology and Life Sciences Blotting, Western Calorimetry Chromatography Funding Genomes HIV-1 - physiology Immunoprecipitation Laboratories Lentivirus Manufacturing Medicine Medicine and Health Sciences Microscopy Microscopy, Electron Physical Sciences Protein Precursors - chemistry Proteins Research and Analysis Methods Retroviridae RNA, Viral - chemistry Thermodynamics Virus Assembly - physiology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The interactions that occur during HIV Pr55Gag oligomerization and genomic RNA packaging are essential elements that facilitate HIV assembly. However, mechanistic details of these interactions are not clearly defined. Here, we overcome previous limitations in producing large quantities of full-length recombinant Pr55Gag that is required for isothermal titration calorimetry (ITC) studies, and we have revealed the thermodynamic properties of HIV assembly for the first time. Thermodynamic analysis showed that the binding between RNA and HIV Pr55Gag is an energetically favourable reaction (ΔG
ISSN:	1553-7374 1553-7366 1553-7374
DOI:	10.1371/journal.ppat.1006221