Identification of Gastric Cancer Biomarkers Using 1H Nuclear Magnetic Resonance Spectrometry

Existing gastric cancer diagnosing methods were invasive, hence, a reliable non-invasive gastric cancer diagnosing method is needed. As a starting point, we used 1H NMR for identifying gastric cancer biomarkers using a panel of gastric cancer spheroids and normal gastric spheroids. We were able to i...

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Veröffentlicht in:	PloS one 2016-09, Vol.11 (9), p.e0162222
Hauptverfasser:	Ramachandran, Gokula Krishnan, Yong, Wei Peng, Yeow, Chen Hua
Format:	Artikel
Sprache:	eng
Schlagworte:	Bioindicators Biology and Life Sciences Biomarkers Biomarkers, Tumor - analysis Biomedical engineering Biopsy Cancer Cell Line, Tumor Chemistry Chemotherapy Endoscopy Energy metabolism Feasibility Studies Gallbladder diseases Gastric cancer Gene expression Glycosylation Humans Lipid composition Lipid metabolism Lipids Magnetic resonance Magnetic resonance imaging Magnetic Resonance Spectroscopy Medical diagnosis Medical prognosis Medical research Medicine and Health Sciences Metabolism Metastasis Mortality Neurosciences NMR Nuclear magnetic resonance Phospholipids - metabolism Physical Sciences Prostate cancer Research and analysis methods Scientific imaging Signal transduction Spectrometry Spheroids Spheroids, Cellular Stomach cancer Stomach Neoplasms - diagnosis Studies
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Beschreibung
Zusammenfassung:	Existing gastric cancer diagnosing methods were invasive, hence, a reliable non-invasive gastric cancer diagnosing method is needed. As a starting point, we used 1H NMR for identifying gastric cancer biomarkers using a panel of gastric cancer spheroids and normal gastric spheroids. We were able to identify 8 chemical shift biomarkers for gastric cancer spheroids. Our data suggests that the cancerous and non-cancerous spheroids significantly differ in the lipid composition and energy metabolism. These results encourage the translation of these biomarkers into in-vivo gastric cancer detection methodology using MRI-MS.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0162222