In Vitro and In Vivo Miltefosine Susceptibility of a Leishmania amazonensis Isolate from a Patient with Diffuse Cutaneous Leishmaniasis: Follow-Up

Histopathological examinations of the infection site and limiting dilution assays performed with the footpad tissue revealed an absence of parasites [5]. The relapses observed in this group of mice indicate that the techniques used in our previous report to characterize the parasite burden (histopathology and limiting dilution) lack the sensitivity to detect these remaining or quiescent parasites.