High-Resolution Denitrification Kinetics in Pasture Soils Link N2O Emissions to pH, and Denitrification to C Mineralization

Denitrification in pasture soils is mediated by microbial and physicochemical processes leading to nitrogen loss through the emission of N2O and N2. It is known that N2O reduction to N2 is impaired by low soil pH yet controversy remains as inconsistent use of soil pH measurement methods by researche...

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Veröffentlicht in:PloS one 2016-03, Vol.11 (3), p.e0151713-e0151713
Hauptverfasser: Samad, Md Sainur, Bakken, Lars R, Nadeem, Shahid, Clough, Timothy J, de Klein, Cecile A M, Richards, Karl G, Lanigan, Gary J, Morales, Sergio E
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container_title PloS one
container_volume 11
creator Samad, Md Sainur
Bakken, Lars R
Nadeem, Shahid
Clough, Timothy J
de Klein, Cecile A M
Richards, Karl G
Lanigan, Gary J
Morales, Sergio E
description Denitrification in pasture soils is mediated by microbial and physicochemical processes leading to nitrogen loss through the emission of N2O and N2. It is known that N2O reduction to N2 is impaired by low soil pH yet controversy remains as inconsistent use of soil pH measurement methods by researchers, and differences in analytical methods between studies, undermine direct comparison of results. In addition, the link between denitrification and N2O emissions in response to carbon (C) mineralization and pH in different pasture soils is still not well described. We hypothesized that potential denitrification rate and aerobic respiration rate would be positively associated with soils. This relationship was predicted to be more robust when a high resolution analysis is performed as opposed to a single time point comparison. We tested this by characterizing 13 different temperate pasture soils from northern and southern hemispheres sites (Ireland and New Zealand) using a fully automated-high-resolution GC detection system that allowed us to detect a wide range of gas emissions simultaneously. We also compared the impact of using different extractants for determining pH on our conclusions. In all pH measurements, soil pH was strongly and negatively associated with both N2O production index (IN2O) and N2O/(N2O+N2) product ratio. Furthermore, emission kinetics across all soils revealed that the denitrification rates under anoxic conditions (NO+N2O+N2 μmol N/h/vial) were significantly associated with C mineralization (CO2 μmol/h/vial) measured both under oxic (r2 = 0.62, p = 0.0015) and anoxic (r2 = 0.89, p
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subjects Acidification
Aerobic respiration
Anaerobiosis - physiology
Analytical methods
Anoxic conditions
Biology and Life Sciences
Carbon
Carbon - metabolism
Carbon dioxide
Denitrification
Denitrification - physiology
Earth sciences
Ecology and Environmental Sciences
Emissions
Environmental conditions
Environmental science
Enzymes
Extractants
Hemispheres
High resolution
Immunology
Kinetics
Life sciences
Measurement methods
Microorganisms
Mineralization
Nitrates
Nitric Oxide - metabolism
Nitrogen
Nitrogen - metabolism
Nitrous oxide
Nitrous Oxide - metabolism
Nitrous oxides
Oxygen - metabolism
Pasture
pH effects
Physical Sciences
Physicochemical processes
Soil - chemistry
Soil Microbiology
Soil pH
Soils
title High-Resolution Denitrification Kinetics in Pasture Soils Link N2O Emissions to pH, and Denitrification to C Mineralization
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