By Regulating Mitochondrial Ca2+-Uptake UCP2 Modulates Intracellular Ca2

The possible role of UCP2 in modulating mitochondrial Ca2+-uptake (mCa2+-uptake) via the mitochondrial calcium uniporter (MCU) is highly controversial. Thus, we analyzed mCa2+-uptake in isolated cardiac mitochondria, MCU single-channel activity in cardiac mitoplasts, dual Ca2+-transients from mitoch...

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Veröffentlicht in:	PloS one 2016-02, Vol.11 (2), p.e0148359-e0148359
Hauptverfasser:	Motloch, Lukas Jaroslaw, Larbig, Robert, Gebing, Tina, Reda, Sara, Schwaiger, Astrid, Leitner, Johannes, Wolny, Martin, Eckardt, Lars, Hoppe, Uta C
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - metabolism Adenosine Triphosphate - pharmacology Animals ATP Biology and Life Sciences Calcium Calcium (intracellular) Calcium (mitochondrial) Calcium - metabolism Calcium channels (voltage-gated) Calcium Channels - metabolism Calcium influx Calcium sequestration Cardiomyocytes Channel gating Contraction Cytosol - drug effects Cytosol - metabolism Dose-Response Relationship, Drug Endoplasmic reticulum Engineering and Technology Experiments Heart Heart diseases Internal medicine Intracellular Ion Channels - genetics Ion Channels - metabolism Laboratory animals Male Medicine Medicine and Health Sciences Membrane Potential, Mitochondrial Mice, Mutant Strains Mitochondria Mitochondria, Heart - drug effects Mitochondria, Heart - metabolism Mitochondrial Proteins - genetics Mitochondrial Proteins - metabolism Mitochondrial uncoupling protein 2 Myocytes, Cardiac - metabolism Ostomy Patch-Clamp Techniques Physical Sciences Proteins Research and analysis methods Rodents Ruthenium Compounds - administration & dosage Ruthenium Compounds - pharmacology Sarcolemma - metabolism Uncoupling Protein 2
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Zusammenfassung:	The possible role of UCP2 in modulating mitochondrial Ca2+-uptake (mCa2+-uptake) via the mitochondrial calcium uniporter (MCU) is highly controversial. Thus, we analyzed mCa2+-uptake in isolated cardiac mitochondria, MCU single-channel activity in cardiac mitoplasts, dual Ca2+-transients from mitochondrial ((Ca2+)m) and intracellular compartment ((Ca2+)c) in the whole-cell configuration in cardiomyocytes of wild-type (WT) and UCP2-/- mice. Isolated mitochondria showed a Ru360 sensitive mCa2+-uptake, which was significantly decreased in UCP2-/- (229.4±30.8 FU vs. 146.3±23.4 FU, P
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0148359