Late-Onset Bloodstream Infection and Perturbed Maturation of the Gastrointestinal Microbiota in Premature Infants
Late-onset bloodstream infection (LO-BSI) is a common complication of prematurity, and lack of timely diagnosis and treatment can have life-threatening consequences. We sought to identify clinical characteristics and microbial signatures in the gastrointestinal microbiota preceding diagnosis of LO-B...
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| Veröffentlicht in: | PloS one 2015-07, Vol.10 (7), p.e0132923-e0132923 |
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| creator | Shaw, Alexander G Sim, Kathleen Randell, Paul Cox, Michael J McClure, Zoë E Li, Ming-Shi Donaldson, Hugo Langford, Paul R Cookson, William O C M Moffatt, Miriam F Kroll, J Simon |
| description | Late-onset bloodstream infection (LO-BSI) is a common complication of prematurity, and lack of timely diagnosis and treatment can have life-threatening consequences. We sought to identify clinical characteristics and microbial signatures in the gastrointestinal microbiota preceding diagnosis of LO-BSI in premature infants.
Daily faecal samples and clinical data were collected over two years from 369 premature neonates ( |
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Daily faecal samples and clinical data were collected over two years from 369 premature neonates (<32 weeks gestation). We analysed samples from 22 neonates who developed LO-BSI and 44 matched control infants. Next-generation sequencing of 16S rRNA gene regions amplified by PCR from total faecal DNA was used to characterise the microbiota of faecal samples preceding diagnosis from infants with LO-BSI and controls. Culture of selected samples was undertaken, and bacterial isolates identified using MALDI-TOF. Antibiograms from bloodstream and faecal isolates were compared to explore strain similarity.
From the week prior to diagnosis, infants with LO-BSI had higher proportions of faecal aerobes/facultative anaerobes compared to controls. Risk factors for LO-BSI were identified by multivariate analysis. Enterobacteriaceal sepsis was associated with antecedent multiple lines, low birth weight and a faecal microbiota with prominent Enterobacteriaceae. Staphylococcal sepsis was associated with Staphylococcus OTU faecal over-abundance, and the number of days prior to diagnosis of mechanical ventilation and of the presence of centrally-placed lines. In 12 cases, the antibiogram of the bloodstream isolate matched that of a component of the faecal microbiota in the sample collected closest to diagnosis.
The gastrointestinal tract is an important reservoir for LO-BSI organisms, pathogens translocating across the epithelial barrier. LO-BSI is associated with an aberrant microbiota, with abundant staphylococci and Enterobacteriaceae and a failure to mature towards predominance of obligate anaerobes.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0132923</identifier><identifier>PMID: 26167683</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aberration ; Aerobes ; Anaerobes ; Analysis ; Antibiotics ; Babies ; Bacteria ; Birth weight ; Deoxyribonucleic acid ; Diagnosis ; Disease prevention ; DNA ; Feces - microbiology ; Female ; Gastrointestinal system ; Gastrointestinal tract ; Gastrointestinal Tract - microbiology ; Gene sequencing ; Genomics ; Gestation ; Health aspects ; Humans ; Infant, Newborn ; Infant, Premature ; Infants ; Infection ; Infections ; Infectious diseases ; Low birth weight ; Male ; Maturation ; Mechanical ventilation ; Medicine ; Microbiota ; Microbiota (Symbiotic organisms) ; Microorganisms ; Multivariate analysis ; Neonates ; Newborn babies ; Nutrition ; Organisms ; Pediatrics ; Physiology ; Premature infants ; Probiotics ; Risk analysis ; Risk factors ; Risk management ; RNA ; rRNA 16S ; Sepsis ; Sepsis - diagnosis ; Sepsis - microbiology ; Staphylococcus aureus ; Ventilation</subject><ispartof>PloS one, 2015-07, Vol.10 (7), p.e0132923-e0132923</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Shaw et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Shaw et al 2015 Shaw et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-79b891b1435067188996cfe0c6601ef46ce0f7c089bf57ab0846cc3dce3c32bd3</citedby><cites>FETCH-LOGICAL-c692t-79b891b1435067188996cfe0c6601ef46ce0f7c089bf57ab0846cc3dce3c32bd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4500406/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4500406/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,2926,23865,27923,27924,53790,53792,79371,79372</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26167683$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Weitkamp, Jörn-Hendrik</contributor><creatorcontrib>Shaw, Alexander G</creatorcontrib><creatorcontrib>Sim, Kathleen</creatorcontrib><creatorcontrib>Randell, Paul</creatorcontrib><creatorcontrib>Cox, Michael J</creatorcontrib><creatorcontrib>McClure, Zoë E</creatorcontrib><creatorcontrib>Li, Ming-Shi</creatorcontrib><creatorcontrib>Donaldson, Hugo</creatorcontrib><creatorcontrib>Langford, Paul R</creatorcontrib><creatorcontrib>Cookson, William O C M</creatorcontrib><creatorcontrib>Moffatt, Miriam F</creatorcontrib><creatorcontrib>Kroll, J Simon</creatorcontrib><title>Late-Onset Bloodstream Infection and Perturbed Maturation of the Gastrointestinal Microbiota in Premature Infants</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Late-onset bloodstream infection (LO-BSI) is a common complication of prematurity, and lack of timely diagnosis and treatment can have life-threatening consequences. We sought to identify clinical characteristics and microbial signatures in the gastrointestinal microbiota preceding diagnosis of LO-BSI in premature infants.
Daily faecal samples and clinical data were collected over two years from 369 premature neonates (<32 weeks gestation). We analysed samples from 22 neonates who developed LO-BSI and 44 matched control infants. Next-generation sequencing of 16S rRNA gene regions amplified by PCR from total faecal DNA was used to characterise the microbiota of faecal samples preceding diagnosis from infants with LO-BSI and controls. Culture of selected samples was undertaken, and bacterial isolates identified using MALDI-TOF. Antibiograms from bloodstream and faecal isolates were compared to explore strain similarity.
From the week prior to diagnosis, infants with LO-BSI had higher proportions of faecal aerobes/facultative anaerobes compared to controls. Risk factors for LO-BSI were identified by multivariate analysis. Enterobacteriaceal sepsis was associated with antecedent multiple lines, low birth weight and a faecal microbiota with prominent Enterobacteriaceae. Staphylococcal sepsis was associated with Staphylococcus OTU faecal over-abundance, and the number of days prior to diagnosis of mechanical ventilation and of the presence of centrally-placed lines. In 12 cases, the antibiogram of the bloodstream isolate matched that of a component of the faecal microbiota in the sample collected closest to diagnosis.
The gastrointestinal tract is an important reservoir for LO-BSI organisms, pathogens translocating across the epithelial barrier. LO-BSI is associated with an aberrant microbiota, with abundant staphylococci and Enterobacteriaceae and a failure to mature towards predominance of obligate anaerobes.</description><subject>Aberration</subject><subject>Aerobes</subject><subject>Anaerobes</subject><subject>Analysis</subject><subject>Antibiotics</subject><subject>Babies</subject><subject>Bacteria</subject><subject>Birth weight</subject><subject>Deoxyribonucleic acid</subject><subject>Diagnosis</subject><subject>Disease prevention</subject><subject>DNA</subject><subject>Feces - microbiology</subject><subject>Female</subject><subject>Gastrointestinal system</subject><subject>Gastrointestinal tract</subject><subject>Gastrointestinal Tract - microbiology</subject><subject>Gene sequencing</subject><subject>Genomics</subject><subject>Gestation</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Infant, Premature</subject><subject>Infants</subject><subject>Infection</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Low birth weight</subject><subject>Male</subject><subject>Maturation</subject><subject>Mechanical ventilation</subject><subject>Medicine</subject><subject>Microbiota</subject><subject>Microbiota (Symbiotic organisms)</subject><subject>Microorganisms</subject><subject>Multivariate analysis</subject><subject>Neonates</subject><subject>Newborn babies</subject><subject>Nutrition</subject><subject>Organisms</subject><subject>Pediatrics</subject><subject>Physiology</subject><subject>Premature infants</subject><subject>Probiotics</subject><subject>Risk analysis</subject><subject>Risk factors</subject><subject>Risk management</subject><subject>RNA</subject><subject>rRNA 16S</subject><subject>Sepsis</subject><subject>Sepsis - 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microbiology</topic><topic>Female</topic><topic>Gastrointestinal system</topic><topic>Gastrointestinal tract</topic><topic>Gastrointestinal Tract - microbiology</topic><topic>Gene sequencing</topic><topic>Genomics</topic><topic>Gestation</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Infant, Premature</topic><topic>Infants</topic><topic>Infection</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Low birth weight</topic><topic>Male</topic><topic>Maturation</topic><topic>Mechanical ventilation</topic><topic>Medicine</topic><topic>Microbiota</topic><topic>Microbiota (Symbiotic organisms)</topic><topic>Microorganisms</topic><topic>Multivariate analysis</topic><topic>Neonates</topic><topic>Newborn babies</topic><topic>Nutrition</topic><topic>Organisms</topic><topic>Pediatrics</topic><topic>Physiology</topic><topic>Premature infants</topic><topic>Probiotics</topic><topic>Risk analysis</topic><topic>Risk factors</topic><topic>Risk management</topic><topic>RNA</topic><topic>rRNA 16S</topic><topic>Sepsis</topic><topic>Sepsis - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shaw, Alexander G</au><au>Sim, Kathleen</au><au>Randell, Paul</au><au>Cox, Michael J</au><au>McClure, Zoë E</au><au>Li, Ming-Shi</au><au>Donaldson, Hugo</au><au>Langford, Paul R</au><au>Cookson, William O C M</au><au>Moffatt, Miriam F</au><au>Kroll, J Simon</au><au>Weitkamp, Jörn-Hendrik</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Late-Onset Bloodstream Infection and Perturbed Maturation of the Gastrointestinal Microbiota in Premature Infants</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-07-13</date><risdate>2015</risdate><volume>10</volume><issue>7</issue><spage>e0132923</spage><epage>e0132923</epage><pages>e0132923-e0132923</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Late-onset bloodstream infection (LO-BSI) is a common complication of prematurity, and lack of timely diagnosis and treatment can have life-threatening consequences. We sought to identify clinical characteristics and microbial signatures in the gastrointestinal microbiota preceding diagnosis of LO-BSI in premature infants.
Daily faecal samples and clinical data were collected over two years from 369 premature neonates (<32 weeks gestation). We analysed samples from 22 neonates who developed LO-BSI and 44 matched control infants. Next-generation sequencing of 16S rRNA gene regions amplified by PCR from total faecal DNA was used to characterise the microbiota of faecal samples preceding diagnosis from infants with LO-BSI and controls. Culture of selected samples was undertaken, and bacterial isolates identified using MALDI-TOF. Antibiograms from bloodstream and faecal isolates were compared to explore strain similarity.
From the week prior to diagnosis, infants with LO-BSI had higher proportions of faecal aerobes/facultative anaerobes compared to controls. Risk factors for LO-BSI were identified by multivariate analysis. Enterobacteriaceal sepsis was associated with antecedent multiple lines, low birth weight and a faecal microbiota with prominent Enterobacteriaceae. Staphylococcal sepsis was associated with Staphylococcus OTU faecal over-abundance, and the number of days prior to diagnosis of mechanical ventilation and of the presence of centrally-placed lines. In 12 cases, the antibiogram of the bloodstream isolate matched that of a component of the faecal microbiota in the sample collected closest to diagnosis.
The gastrointestinal tract is an important reservoir for LO-BSI organisms, pathogens translocating across the epithelial barrier. LO-BSI is associated with an aberrant microbiota, with abundant staphylococci and Enterobacteriaceae and a failure to mature towards predominance of obligate anaerobes.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26167683</pmid><doi>10.1371/journal.pone.0132923</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2015-07, Vol.10 (7), p.e0132923-e0132923 |
| issn | 1932-6203 1932-6203 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Aberration Aerobes Anaerobes Analysis Antibiotics Babies Bacteria Birth weight Deoxyribonucleic acid Diagnosis Disease prevention DNA Feces - microbiology Female Gastrointestinal system Gastrointestinal tract Gastrointestinal Tract - microbiology Gene sequencing Genomics Gestation Health aspects Humans Infant, Newborn Infant, Premature Infants Infection Infections Infectious diseases Low birth weight Male Maturation Mechanical ventilation Medicine Microbiota Microbiota (Symbiotic organisms) Microorganisms Multivariate analysis Neonates Newborn babies Nutrition Organisms Pediatrics Physiology Premature infants Probiotics Risk analysis Risk factors Risk management RNA rRNA 16S Sepsis Sepsis - diagnosis Sepsis - microbiology Staphylococcus aureus Ventilation |
| title | Late-Onset Bloodstream Infection and Perturbed Maturation of the Gastrointestinal Microbiota in Premature Infants |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T07%3A37%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Late-Onset%20Bloodstream%20Infection%20and%20Perturbed%20Maturation%20of%20the%20Gastrointestinal%20Microbiota%20in%20Premature%20Infants&rft.jtitle=PloS%20one&rft.au=Shaw,%20Alexander%20G&rft.date=2015-07-13&rft.volume=10&rft.issue=7&rft.spage=e0132923&rft.epage=e0132923&rft.pages=e0132923-e0132923&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0132923&rft_dat=%3Cgale_plos_%3EA421593564%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1695995711&rft_id=info:pmid/26167683&rft_galeid=A421593564&rft_doaj_id=oai_doaj_org_article_a316d48b9fb04e33bec7607229731e69&rfr_iscdi=true |