High-Throughput Sequencing Identifies Novel and Conserved Cucumber (Cucumis sativus L.) microRNAs in Response to Cucumber Green Mottle Mosaic Virus Infection

Seedlings of Cucumis sativus L. (cv. 'Zhongnong 16') were artificially inoculated with Cucumber green mottle mosaic virus (CGMMV) at the three-true-leaf stage. Leaf and flower samples were collected at different time points post-inoculation (10, 30 and 50 d), and processed by high throughp...

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Veröffentlicht in:	PloS one 2015-06, Vol.10 (6), p.e0129002-e0129002
Hauptverfasser:	Liu, H W, Luo, L X, Liang, C Q, Jiang, N, Liu, P F, Li, J Q
Format:	Artikel
Sprache:	eng
Schlagworte:	Abiotic stress Algorithms Bioinformatics Biological activity Biosynthesis Computational Biology Cucumbers Cucumis sativus Cucumis sativus - genetics Cucumis sativus - immunology Cucumis sativus - virology Disease Disease Resistance - genetics DNA methylation Encyclopedias Engineering research Gene expression Genes Genomes High-Throughput Nucleotide Sequencing Host-pathogen interactions Infections Information processing Inoculation Laboratories Leaves Metabolism Metabolites MicroRNAs MicroRNAs - chemistry MicroRNAs - genetics MicroRNAs - metabolism miRNA Molecular chains Next-generation sequencing Oligonucleotide Array Sequence Analysis Pathogens Plant biology Plant Diseases - genetics Plant Diseases - immunology Plant Diseases - virology Plant pathology Plant virus diseases Predictions Protein structure Proteins Ribonucleic acid Rice RNA RNA polymerase RNA, Plant - chemistry RNA, Plant - genetics RNA, Plant - metabolism Screening Secondary metabolites Secondary structure Seedlings Seeds Sequence Analysis, RNA Signal transduction Tobamovirus - physiology Trends Viruses
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Zusammenfassung:	Seedlings of Cucumis sativus L. (cv. 'Zhongnong 16') were artificially inoculated with Cucumber green mottle mosaic virus (CGMMV) at the three-true-leaf stage. Leaf and flower samples were collected at different time points post-inoculation (10, 30 and 50 d), and processed by high throughput sequencing analysis to identify candidate miRNA sequences. Bioinformatic analysis using screening criteria, and secondary structure prediction, indicated that 8 novel and 23 known miRNAs (including 15 miRNAs described for the first time in vivo) were produced by cucumber plants in response to CGMMV infection. Moreover, gene expression profiles (p-value
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0129002