MicroRNA-21 Regulates PI3K/Akt/mTOR Signaling by Targeting TGFβI during Skeletal Muscle Development in Pigs

MicroRNAs (miRNAs), which are short (22-24 base pairs), non-coding RNAs, play critical roles in myogenesis. Using Solexa deep sequencing, we detected the expression levels of 229 and 209 miRNAs in swine skeletal muscle at 90 days post-coitus (E90) and 100 days postnatal (D100), respectively. A total...

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Veröffentlicht in:	PloS one 2015-05, Vol.10 (5), p.e0119396-e0119396
Hauptverfasser:	Bai, Lijing, Liang, Ruyi, Yang, Yalan, Hou, Xinhua, Wang, Zishuai, Zhu, Shiyun, Wang, Chuduan, Tang, Zhonglin, Li, Kui
Format:	Artikel
Sprache:	eng
Schlagworte:	1-Phosphatidylinositol 3-kinase Agriculture AKT protein Animal sciences Animals Base pairs Bioinformatics Breeding of animals Cells, Cultured Developmental stages Gene expression Gene Expression Regulation, Developmental Genomes Germplasm Growth factors Hogs Laboratories Livestock MicroRNAs MicroRNAs - genetics MicroRNAs - metabolism miRNA Molecular Sequence Data Muscle, Skeletal - growth & development Muscle, Skeletal - metabolism Muscles Musculoskeletal system Myogenesis Nutrition Phosphatidylinositol 3-Kinases - metabolism Polymerase chain reaction Proto-Oncogene Proteins c-akt - metabolism Pulmonary arteries Ribonucleic acid RNA Sequence Analysis, RNA Sexual intercourse Signal Transduction Signaling Skeletal muscle Smooth muscle Studies Suidae Swine TOR protein TOR Serine-Threonine Kinases - metabolism Transforming Growth Factor beta - genetics Transforming Growth Factor beta - metabolism Transforming growth factor-b Western blotting Zoology
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Zusammenfassung:	MicroRNAs (miRNAs), which are short (22-24 base pairs), non-coding RNAs, play critical roles in myogenesis. Using Solexa deep sequencing, we detected the expression levels of 229 and 209 miRNAs in swine skeletal muscle at 90 days post-coitus (E90) and 100 days postnatal (D100), respectively. A total of 138 miRNAs were up-regulated on E90, and 31 were up-regulated on D100. Of these, 9 miRNAs were selected for the validation of the small RNA libraries by quantitative RT-PCR (RT-qPCR). We found that miRNA-21 was down-regulated by 17-fold on D100 (P
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0119396