The repertoire of archaea cultivated from severe periodontitis
In previous studies, the abundance and diversity of methanogenic archaea in the dental microbiota have been analysed by the detection of specific DNA sequences by PCR-based investigations and metagenomic studies. Few data issued regarding methanogens actually living in dental plaque. We collected de...
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| Veröffentlicht in: | PloS one 2015-04, Vol.10 (4), p.e0121565-e0121565 |
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| creator | Huynh, Hong T T Pignoly, Marion Nkamga, Vanessa D Drancourt, Michel Aboudharam, Gérard |
| description | In previous studies, the abundance and diversity of methanogenic archaea in the dental microbiota have been analysed by the detection of specific DNA sequences by PCR-based investigations and metagenomic studies. Few data issued regarding methanogens actually living in dental plaque. We collected dental plaque specimens in 15 control individuals and 65 periodontitis patients. Dental plaque specimens were cultured in an anoxic liquid medium for methanogens in the presence of negative control tubes. Dental plaque methanogens were cultured from 1/15 (6.67%) control and 36/65 (55.38%) periodontitis patient samples (p |
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Few data issued regarding methanogens actually living in dental plaque. We collected dental plaque specimens in 15 control individuals and 65 periodontitis patients. Dental plaque specimens were cultured in an anoxic liquid medium for methanogens in the presence of negative control tubes. Dental plaque methanogens were cultured from 1/15 (6.67%) control and 36/65 (55.38%) periodontitis patient samples (p<0.001). The cultures yielded Methanobrevibacter oralis in one control and thirty-one patients, Methanobrevibacter smithii in two patients and a potential new species named Methanobrevibacter sp. strain N13 in three patients with severe periodontitis. Our observations of living methanogens, strengthen previous observations made on DNA-based studies regarding the role of methanogens, in periodontitis.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0121565</identifier><identifier>PMID: 25830311</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adult ; Aged ; Archaea ; Case-Control Studies ; Dental plaque ; Dental Plaque - microbiology ; Deoxyribonucleic acid ; DNA ; DNA, Archaeal - genetics ; Female ; Gene sequencing ; Gum disease ; Humans ; Male ; Methanobrevibacter - genetics ; Methanobrevibacter - isolation & purification ; Methanobrevibacter oralis ; Methanobrevibacter smithii ; Methanogenic archaea ; Methanogenic bacteria ; Microbiota ; Middle Aged ; Molecular Sequence Data ; Molecular Typing ; New species ; Nucleotide sequence ; Patients ; Periodontitis ; Periodontitis - microbiology ; Phylogeny ; RNA, Ribosomal, 16S - genetics ; Young Adult</subject><ispartof>PloS one, 2015-04, Vol.10 (4), p.e0121565-e0121565</ispartof><rights>2015 Huynh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Huynh et al 2015 Huynh et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-8ca801b6a0f31e9135ea39dca8f5f5efafb46e34f9fb0208764d70e6fd1ab4613</citedby><cites>FETCH-LOGICAL-c526t-8ca801b6a0f31e9135ea39dca8f5f5efafb46e34f9fb0208764d70e6fd1ab4613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4382158/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4382158/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23870,27928,27929,53795,53797</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25830311$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Moissl-Eichinger, Christine</contributor><creatorcontrib>Huynh, Hong T T</creatorcontrib><creatorcontrib>Pignoly, Marion</creatorcontrib><creatorcontrib>Nkamga, Vanessa D</creatorcontrib><creatorcontrib>Drancourt, Michel</creatorcontrib><creatorcontrib>Aboudharam, Gérard</creatorcontrib><title>The repertoire of archaea cultivated from severe periodontitis</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>In previous studies, the abundance and diversity of methanogenic archaea in the dental microbiota have been analysed by the detection of specific DNA sequences by PCR-based investigations and metagenomic studies. Few data issued regarding methanogens actually living in dental plaque. We collected dental plaque specimens in 15 control individuals and 65 periodontitis patients. Dental plaque specimens were cultured in an anoxic liquid medium for methanogens in the presence of negative control tubes. Dental plaque methanogens were cultured from 1/15 (6.67%) control and 36/65 (55.38%) periodontitis patient samples (p<0.001). The cultures yielded Methanobrevibacter oralis in one control and thirty-one patients, Methanobrevibacter smithii in two patients and a potential new species named Methanobrevibacter sp. strain N13 in three patients with severe periodontitis. Our observations of living methanogens, strengthen previous observations made on DNA-based studies regarding the role of methanogens, in periodontitis.</description><subject>Adult</subject><subject>Aged</subject><subject>Archaea</subject><subject>Case-Control Studies</subject><subject>Dental plaque</subject><subject>Dental Plaque - microbiology</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Archaeal - genetics</subject><subject>Female</subject><subject>Gene sequencing</subject><subject>Gum disease</subject><subject>Humans</subject><subject>Male</subject><subject>Methanobrevibacter - genetics</subject><subject>Methanobrevibacter - isolation & purification</subject><subject>Methanobrevibacter oralis</subject><subject>Methanobrevibacter smithii</subject><subject>Methanogenic archaea</subject><subject>Methanogenic bacteria</subject><subject>Microbiota</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>Molecular Typing</subject><subject>New species</subject><subject>Nucleotide sequence</subject><subject>Patients</subject><subject>Periodontitis</subject><subject>Periodontitis - 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Few data issued regarding methanogens actually living in dental plaque. We collected dental plaque specimens in 15 control individuals and 65 periodontitis patients. Dental plaque specimens were cultured in an anoxic liquid medium for methanogens in the presence of negative control tubes. Dental plaque methanogens were cultured from 1/15 (6.67%) control and 36/65 (55.38%) periodontitis patient samples (p<0.001). The cultures yielded Methanobrevibacter oralis in one control and thirty-one patients, Methanobrevibacter smithii in two patients and a potential new species named Methanobrevibacter sp. strain N13 in three patients with severe periodontitis. Our observations of living methanogens, strengthen previous observations made on DNA-based studies regarding the role of methanogens, in periodontitis.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25830311</pmid><doi>10.1371/journal.pone.0121565</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Aged Archaea Case-Control Studies Dental plaque Dental Plaque - microbiology Deoxyribonucleic acid DNA DNA, Archaeal - genetics Female Gene sequencing Gum disease Humans Male Methanobrevibacter - genetics Methanobrevibacter - isolation & purification Methanobrevibacter oralis Methanobrevibacter smithii Methanogenic archaea Methanogenic bacteria Microbiota Middle Aged Molecular Sequence Data Molecular Typing New species Nucleotide sequence Patients Periodontitis Periodontitis - microbiology Phylogeny RNA, Ribosomal, 16S - genetics Young Adult |
| title | The repertoire of archaea cultivated from severe periodontitis |
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