The repertoire of archaea cultivated from severe periodontitis

In previous studies, the abundance and diversity of methanogenic archaea in the dental microbiota have been analysed by the detection of specific DNA sequences by PCR-based investigations and metagenomic studies. Few data issued regarding methanogens actually living in dental plaque. We collected de...

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Veröffentlicht in:	PloS one 2015-04, Vol.10 (4), p.e0121565-e0121565
Hauptverfasser:	Huynh, Hong T T, Pignoly, Marion, Nkamga, Vanessa D, Drancourt, Michel, Aboudharam, Gérard
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Aged Archaea Case-Control Studies Dental plaque Dental Plaque - microbiology Deoxyribonucleic acid DNA DNA, Archaeal - genetics Female Gene sequencing Gum disease Humans Male Methanobrevibacter - genetics Methanobrevibacter - isolation & purification Methanobrevibacter oralis Methanobrevibacter smithii Methanogenic archaea Methanogenic bacteria Microbiota Middle Aged Molecular Sequence Data Molecular Typing New species Nucleotide sequence Patients Periodontitis Periodontitis - microbiology Phylogeny RNA, Ribosomal, 16S - genetics Young Adult
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Zusammenfassung:	In previous studies, the abundance and diversity of methanogenic archaea in the dental microbiota have been analysed by the detection of specific DNA sequences by PCR-based investigations and metagenomic studies. Few data issued regarding methanogens actually living in dental plaque. We collected dental plaque specimens in 15 control individuals and 65 periodontitis patients. Dental plaque specimens were cultured in an anoxic liquid medium for methanogens in the presence of negative control tubes. Dental plaque methanogens were cultured from 1/15 (6.67%) control and 36/65 (55.38%) periodontitis patient samples (p
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0121565