Functional analysis of the engineered cardiac tissue grown on recombinant spidroin fiber meshes
In the present study, we examined the ability of the recombinant spidroin to serve as a substrate for the cardiac tissue engineering. For this purpose, isolated neonatal rat cardiomyocytes were seeded on the electrospun spidroin fiber matrices and cultured to form the confluent cardiac monolayers. B...
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description | In the present study, we examined the ability of the recombinant spidroin to serve as a substrate for the cardiac tissue engineering. For this purpose, isolated neonatal rat cardiomyocytes were seeded on the electrospun spidroin fiber matrices and cultured to form the confluent cardiac monolayers. Besides the adhesion assay and immunostaining analysis, we tested the ability of the cultured cardiomyocytes to form a functional cardiac syncytium by studying excitation propagation in the cultured tissue with the aid of optical mapping. It was demonstrated that recombinant spidroin fiber meshes are directly suitable for the adherence and growth of the cardiomyocytes without additional coating with the attachment factors, such as fibronectin. |
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For this purpose, isolated neonatal rat cardiomyocytes were seeded on the electrospun spidroin fiber matrices and cultured to form the confluent cardiac monolayers. Besides the adhesion assay and immunostaining analysis, we tested the ability of the cultured cardiomyocytes to form a functional cardiac syncytium by studying excitation propagation in the cultured tissue with the aid of optical mapping. It was demonstrated that recombinant spidroin fiber meshes are directly suitable for the adherence and growth of the cardiomyocytes without additional coating with the attachment factors, such as fibronectin.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0121155</identifier><identifier>PMID: 25799394</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adhesion tests ; Animals ; Antheraea ; Biocompatibility ; Biomedical materials ; Cardiomyocytes ; Cell Adhesion ; Cell adhesion & migration ; Cell Proliferation ; Cells, Cultured ; Fibroins - genetics ; Fibroins - metabolism ; Fibronectin ; Fibronectins ; Functional analysis ; Genes ; Growth ; Heart ; Heart diseases ; Microorganisms ; Monomolecular films ; Myocytes, Cardiac - cytology ; Myocytes, Cardiac - metabolism ; Neonates ; Peptides ; Physics ; Physiology ; Proteins ; Rats ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; Saccharomyces cerevisiae ; Silk ; Spiders ; Substrates ; Tissue engineering ; Tissue Engineering - methods ; Tissue Scaffolds</subject><ispartof>PloS one, 2015-03, Vol.10 (3), p.e0121155-e0121155</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Teplenin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Teplenin et al 2015 Teplenin et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-f9f90e76ff2270288521c54a8b333af7b37a57258f26ce75e194746d4edc5c213</citedby><cites>FETCH-LOGICAL-c692t-f9f90e76ff2270288521c54a8b333af7b37a57258f26ce75e194746d4edc5c213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4370870/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4370870/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2929,23868,27926,27927,53793,53795</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25799394$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Pesce, Maurizio</contributor><creatorcontrib>Teplenin, Alexander</creatorcontrib><creatorcontrib>Krasheninnikova, Anna</creatorcontrib><creatorcontrib>Agladze, Nadezhda</creatorcontrib><creatorcontrib>Sidoruk, Konstantin</creatorcontrib><creatorcontrib>Agapova, Olga</creatorcontrib><creatorcontrib>Agapov, Igor</creatorcontrib><creatorcontrib>Bogush, Vladimir</creatorcontrib><creatorcontrib>Agladze, Konstantin</creatorcontrib><title>Functional analysis of the engineered cardiac tissue grown on recombinant spidroin fiber meshes</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>In the present study, we examined the ability of the recombinant spidroin to serve as a substrate for the cardiac tissue engineering. 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It was demonstrated that recombinant spidroin fiber meshes are directly suitable for the adherence and growth of the cardiomyocytes without additional coating with the attachment factors, such as fibronectin.</description><subject>Adhesion tests</subject><subject>Animals</subject><subject>Antheraea</subject><subject>Biocompatibility</subject><subject>Biomedical materials</subject><subject>Cardiomyocytes</subject><subject>Cell Adhesion</subject><subject>Cell adhesion & migration</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Fibroins - genetics</subject><subject>Fibroins - metabolism</subject><subject>Fibronectin</subject><subject>Fibronectins</subject><subject>Functional analysis</subject><subject>Genes</subject><subject>Growth</subject><subject>Heart</subject><subject>Heart diseases</subject><subject>Microorganisms</subject><subject>Monomolecular films</subject><subject>Myocytes, Cardiac - cytology</subject><subject>Myocytes, Cardiac - metabolism</subject><subject>Neonates</subject><subject>Peptides</subject><subject>Physics</subject><subject>Physiology</subject><subject>Proteins</subject><subject>Rats</subject><subject>Recombinant Proteins - 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For this purpose, isolated neonatal rat cardiomyocytes were seeded on the electrospun spidroin fiber matrices and cultured to form the confluent cardiac monolayers. Besides the adhesion assay and immunostaining analysis, we tested the ability of the cultured cardiomyocytes to form a functional cardiac syncytium by studying excitation propagation in the cultured tissue with the aid of optical mapping. It was demonstrated that recombinant spidroin fiber meshes are directly suitable for the adherence and growth of the cardiomyocytes without additional coating with the attachment factors, such as fibronectin.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25799394</pmid><doi>10.1371/journal.pone.0121155</doi><oa>free_for_read</oa></addata></record> |
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subjects | Adhesion tests Animals Antheraea Biocompatibility Biomedical materials Cardiomyocytes Cell Adhesion Cell adhesion & migration Cell Proliferation Cells, Cultured Fibroins - genetics Fibroins - metabolism Fibronectin Fibronectins Functional analysis Genes Growth Heart Heart diseases Microorganisms Monomolecular films Myocytes, Cardiac - cytology Myocytes, Cardiac - metabolism Neonates Peptides Physics Physiology Proteins Rats Recombinant Proteins - genetics Recombinant Proteins - metabolism Saccharomyces cerevisiae Silk Spiders Substrates Tissue engineering Tissue Engineering - methods Tissue Scaffolds |
title | Functional analysis of the engineered cardiac tissue grown on recombinant spidroin fiber meshes |
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